This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, H.
Right arrow Articles by Rudd, C. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, H.
Right arrow Articles by Rudd, C. E.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2007, p. 6863-6875, Vol. 27, No. 19
0270-7306/07/$08.00+0     doi:10.1128/MCB.00556-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Functional Defects of SKAP-55-Deficient T Cells Identify a Regulatory Role for the Adaptor in LFA-1 Adhesion{triangledown}

Hongyan Wang,1,2 Hebin Liu,1,2 Yuning Lu,1,2 Matt Lovatt,2 Bin Wei,2 and Christopher E. Rudd1,2*

Cell Signalling Section, Division of Immunology, Department of Pathology, Tennis Court Road, University of Cambridge, Cambridge CB2 1QP,1 Molecular Immunology Section, Department of Immunology, Imperial College London, Hammersmith Hospital, London W12 0NN, United Kingdom2

Received 29 March 2007/ Returned for modification 1 May 2007/ Accepted 17 July 2007


arrow
ABSTRACT
 
The ADAP-SKAP-55 module regulates T-cell receptor (TCR)-induced integrin clustering and adhesion in T cells. However, it has been unclear whether ADAP and/or SKAP-55 is an effector of the response. ADAP controls SKAP-55 expression such that ADAP–/– T cells are also deficient in SKAP-55 expression. In this study, we report the phenotype of the SKAP-55-deficient mouse. SKAP-55–/– T cells retain ADAP expression yet show defects in ß1 and ß2 integrin adhesion, leukocyte function-associated antigen 1 (LFA-1) clustering, production of the cytokines interleukin-2 and gamma interferon, and proliferation. This dependency was also reflected in more-transient conjugation times in response to the superantigen staphylococcal enterotoxin A on dendritic cells and a reduced number of cells with TCR/CD3 microcluster localization at the immunological synapse. SKAP-55–/– T cells showed the same general impairment of function as ADAP–/– T cells, indicating that SKAP-55 is an effector of the ADAP-SKAP-55 module. At the same time, the requirement for ADAP and SKAP-55 was not absolute, since a subset of peripheral T cells adhered with loss of expression of either adaptor. Further, dependency on SKAP-55 or ADAP differed with the strength of the TCR signal. As with the ADAP–/– mouse, SKAP-55-deficient mice showed no major effects on lymphoid development or the appearance of peripheral T cells, B cells, and NK cells. Our findings identify a clear effector role for SKAP-55 in LFA-1 adhesion in peripheral T cells and demonstrate that dependency on SKAP-55 and ADAP differs among T cells and differs with the strength of the TCR signal.


arrow
INTRODUCTION
 
T-cell receptors (TCRs) undergo microclustering and supramolecular activation cluster formation at the immunological synapse (IS) during conjugation between T cells and antigen-presenting cells (APCs) (5, 21, 35). Microclustering can in turn activate GTP-binding proteins, protein kinases, phosphatases, and the phosphorylation of adaptor proteins. CD4- and CD8-p56lck activation leads to immunoreceptor tyrosine-based activation motif phosphorylation on the CD3 and TCR{zeta} chains, the recruitment of ZAP-70 (zeta-associated protein-tyrosine kinase of 70 kDa), and the activation of TEC kinases ITK/RLK (interleukin-2-inducible/resting lymphocyte kinase) (1, 31-33, 41). Adaptor proteins possess binding sites and domains needed for complex-complex formation (31, 32, 41). Immune cell-specific adaptors include LAT (linker for activation of T cells), GADS (Grb-2-like adaptor downstream of Shc), SLP-76 (SH2 domain-containing leukocyte protein of 76 kDa), ADAP (adhesion- and degranulation-promoting adaptor protein; previously known as FYN T-binding protein/SLP-76-associated protein [FYB/SLAP]), and SKAP-55 (Src kinase-associated phosphoprotein of 55 kDa; also known as SCAP1) (3, 23, 24, 26, 31, 32, 40-42). Phosphorylation of LAT recruits phospholipase C{gamma}1, Grb-2, and GADS-SLP-76 and induces Ca2+ mobilization and cytokine transcription (32, 33, 41, 44).

Binding of leukocyte function-associated antigen 1 (LFA-1; also known as CD11/CD18 or {alpha}Lß2) to intercellular adhesion molecules 1 and 2 (ICAM-1 and -2) on APCs mediates T-cell-APC conjugation (5, 8, 11, 21, 37). Following initial adhesion, TCR{zeta}/CD3 ligation induces signals (i.e., "inside-out signaling") that further activate integrin adhesion (2, 5, 8, 11, 37). Conversion of LFA-1 to intermediate- or higher-affinity forms involves changes in conformation and receptor clustering (11, 37). Multiple signaling proteins mediate this process. They include the GTP-binding protein Rap-1, its ligand RapL (regulator of cell adhesion and polarization enriched in lymphoid tissues), RIAM (Rap1-GTP-interacting adaptor molecule), the guanine nucleotide exchange factor Vav-1, and the adaptors SLP-76, ADAP, and SKAP-55 (6, 10, 13, 15, 16, 17, 18, 20, 22, 27, 28, 34). The protein-tyrosine kinase ZAP-70 phosphorylates YESP sites in SLP-76, which allows binding to the Src homology 2 (SH2) domain of Vav-1 (29), while the SH2 domain of SLP-76 binds to two YDDV sites in ADAP (30, 39). T-cell lines lacking SLP-76 show impaired superantigen-induced conjugation (44).

ADAP is an immune cell-specific adaptor with a unique N-terminal region, a proline-rich region, a canonical and a noncanonical SH3 domain, one Ena/VASP homology 1 (EVH1) binding domain, and two putative nuclear localization motifs (3, 4, 19, 26, 31). ADAP is preferentially phosphorylated by the Src kinase p59fynT (4, 25) and can cooperate with p59fynT and SLP-76 in amplifying TCR-induced interleukin-2 (IL-2) transcription (30). The adaptor can up-regulate integrin-mediated adhesion in certain basophilic cell lines (9), while ADAP–/– T cells show profound defects in ß1 and ß2 integrin clustering and adhesion (10, 27). Binding to SLP-76 is needed, as evidenced by the fact that mutation of the SLP-76 binding sites in ADAP impairs conjugation, supramolecular activation cluster (SMAC) formation, and cytokine production (39). The current model proposes that p59fynT phosphorylates YDDV sites in ADAP that interact with SLP-76 SH2 domains, followed by complex recruitment to LAT via binding of the adaptor GADS to a noncanonical motif in SLP-76 (31, 32, 44).

SKAP-55, another immune cell adaptor, is the main binding partner for ADAP in T cells (14, 23, 24). It is enriched in T cells and possesses a unique N-terminal region followed by a pleckstrin homology domain and a C-terminal SH3 domain (23, 24). SKAP-55 binds with high stoichiometry to ADAP (4, 25), an interaction that is mediated by binding of the SKAP-55 SH3 domain to a proline-rich region in ADAP (23, 25) and by weaker binding of the ADAP SH3c domain to a tyrosine-based RKXXYXXY motif in SKAP-55 (7, 14). SKAP-55 may also interact with the D1 domain of the phosphatase CD45 (43). We have previously shown that SKAP-55 is capable of enhancing TCR-mediated activation of ß1 and ß2 integrins and of forming conjugates between T cells and APCs (40) and that small interfering RNA-mediated knockdown of SKAP-55 impairs conjugate formation and LFA-1 clustering in mouse T cells (13). This effect occurred under conditions of normal expression of the homologue SKAP-55-related (SKAP-55R) or SKAP-55Hom, indicating a nonredundant function for SKAP-55 in T cells (13). An interaction between ADAP and SKAP-55 is needed for adhesion, since the loss of the SKAP- 55 SH3 domain or the ADAP SH3c domain binding sites on SKAP-55 ablates adhesion and conjugate formation (7, 18, 40). ADAP-SKAP-55 may facilitate the translocation of Rap1 to membranes (18).

While ADAP-SKAP-55 comprises a module for integrin adhesion, it has been unclear whether ADAP and/or SKAP-55 is directly responsible for this function (10, 12, 18, 27). An important recent observation has been that ADAP modulates SKAP-55 protein turnover and degradation such that ADAP-deficient cells show a major loss of SKAP-55 expression (12). In this study, we report the phenotype of the SKAP-55-deficient mouse, where T cells lack SKAP-55 but retain ADAP expression. Despite this, SKAP-55-deficient T cells show major defects in integrin adhesion, cytokine production, and proliferation comparable to those for ADAP-deficient T cells. Dwell times of SKAP-55-deficient T cells in response to staphylococcal enterotoxin A (SEA) presented by dendritic cells (DCs) were shorter concurrent with reduced localization of the TCR/CD3 complex at the IS and reduced proliferation. However, the requirement for ADAP-SKAP-55 was not absolute. Adhesion assays and cell cycle analysis identified a subset of T cells that adhere without the need for the adaptors, a dependency that differs with the strength of the TCR signal. Our studies identify an effector function for SKAP-55 that operates independently of ADAP.


arrow
MATERIALS AND METHODS
 
SKAP-55 and ADAP KO mice. SKAP-55 knockout (KO) mice were generated in collaboration with Lexicon Genetics Ltd. (Texas), and the mutagenesis method was based on gene trapping, which allows the automated identification of sequence tags from the mutated genes, as described previously (45). Once germ line transmission had been validated, SKAP-55+/– animals were mated with animals of a C57JBL/6 background, resulting in a 129Sv/lex x C57JBL/6 hybrid background. The SKAP-55 KO line was backcrossed to C57JBL/6 for subsequent progeny. Age- and sex-matched mice from the same litters were used in our experiments. ADAP-deficient mice were kindly provided by Eric Peterson (University of Minnesota).

Cell culture and antibodies. Primary T cells from SKAP-55+/+, SKAP-55+/–, and SKAP-55–/– mice were cultured in RPMI 1640 medium with 10% (vol/vol) fetal calf serum (FCS), 5% (vol/vol) glutamine, 5% (vol/vol) penicillin-streptomycin, and 5 x 10–5 M 2-mercaptoethanol. CD4-positive T cells were purified using anti-CD4 beads as previously described (40). For the generation of bone marrow DCs (BMDCs), bone marrow was flushed from femurs and passed through a 200-µm mesh to remove fibrous tissue, and red blood cells were lysed using ammonium chloride lysis buffer. Cells were cultured at 106/ml in RPMI medium supplemented with 10% FCS, 2 mM glutamine, 50 µM 2-mercaptoethanol, 100 IU/ml penicillin, 100 µg/ml streptomycin, 20 ng/ml recombinant murine granulocyte-macrophage colony-stimulating factor, and 1,000 IU/ml IL-4. On day 5 of culture, floating cells were gently removed and fresh medium containing recombinant murine granulocyte-macrophage colony-stimulating factor was added. On day 7 of culture, BMDCs were induced to mature by addition of 1 µg/ml lipopolysaccharide to the cultures. After overnight incubation, nonadherent cells and loosely adherent proliferating BMDC aggregates were collected, washed, and replated for 1 h at 37°C to remove contaminating macrophages. DCs were then plated with various concentrations of SEA for 2 to 3 h prior to coculture with T cells for proliferation, cytokine, contact time, and imaging analysis. The contact time of individual T-cell-APC conjugates was followed over 1,800 s using MetaMorph imaging software.

Monoclonal antibodies against ADAP and SKAP-55 were purchased from Transduction Laboratories (San Diego, CA), while unconjugated or conjugated antibodies against CD11a (LFA-1, {alpha}L chain), CD18 (LFA-1, ß2 chain), CD4, CD8, CD44, CD25, CD29 (anti-integrin ß1 antibody), CD69, DX5, TCR, and B220 were from Pharmingen (Oxford, United Kingdom). Unconjugated antibodies against mouse CD3 (2C11; hamster anti-mouse CD3) or phosporylated tyrosine (4G10) were prepared and purified by Bioexpress. Alexa Fluor 546- or Alexa Fluor 633-conjugated secondary antibodies were purchased from Molecular Probes (Eugene, OR). Five-domain murine ICAM-1 Fc was purchased from R&D Systems. Antiactin was from Sigma, and an antibody against extracellular signal-regulated kinases 1 and 2 (anti-ERK1/2) was purchased from Cell Signaling [New England Biolabs (UK) Ltd., Hertfordshire, United Kingdom]. Anti-phospho-ERK1/2 T202/Y204 or T185/Y187 was purchased from BioSource United Kingdom (Nivelles, Belgium).

Intracellular staining and CFSE labeling. Intracellular staining and flow cytometry were used to measure the level of antibody against gamma interferon (anti-IFN-{gamma}). After anti-CD3 stimulation, cells were fixed in 4% paraformaldehyde (Sigma) and permeabilized with 0.3% saponin (Sigma), followed by a second staining with allophycocyanin-tagged anti-IFN-{gamma} in saponin containing phosphate-buffered saline (PBS)-bovine serum albumin (BSA). Cells were fixed and analyzed using a FACSCalibur flow cytometer. For carboxyfluorescein diacetate succinimide ester (CFSE) labeling, 5 x 106 cells were washed twice and resuspended in 250 µl of staining buffer (PBS supplemented with 0.1% BSA). A 250-µl volume of freshly diluted 2 µM CFSE (Molecular Probes) was added, and cells were incubated at room temperature for 10 min. An equal volume of FCS was added to stop labeling. After three washes, cells were activated with anti-CD3 for 72 h with a culture medium containing 10% FCS. Cells were harvested and stained with allophycocyanin-conjugated anti-CD4, followed by gating of CD4-positive cells for detection of CFSE fluorescence on a FACSCalibur system (BD Biosciences). Data were analyzed using CellQuest (BD Biosciences).

PCR. PCR amplification was performed in a 50-µl reaction mixture using 1 µl cDNA with 25 µl of 2x Taq master mix and reverse primer B with oligonucleotide primer LTR or primer A (0.5 µM each). The cycling parameters were as follows: 5 min at 95°C for 1 cycle; 1 min at 94°C, 1 min at 65°C, and 1 min and 30 s at 72°C for 10 cycles, with the annealing temperature decreasing 1°C in each cycle; 1 min at 94°C, 1 min at 55°C, and 1 min and 30 s at 72°C for 28 cycles; and a final extension at 72°C for 10 min. After PCR, the amplification reaction product was analyzed on a 2% agarose-ethidium bromide gel.

Immunoblotting. For immunobotting, 4 x 106 cells were lysed in ice-cold lysis buffer containing 1% Triton X-100 (vol/vol) in 20 mM Tris-HCl (pH 8.3), 150 mM NaCl, 1 mM phenylmethylsulfonyl fluoride, 1 mM Na4VO3, 10 mM NaF, and 1 mM Na4P2O7 as described previously (29). Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes. Membranes were then blocked with 5% skim milk in TBS (10 mM Tris-HCl [pH 7.6], 150 mM NaCl) and incubated for 1 h with the indicated antibodies. Levels of bound antibody were then measured using a horseradish peroxidase-conjugated rabbit anti-mouse secondary antibody, followed by detection with enhanced chemiluminescence (ECL; Amersham Pharmacia Biotech, Piscataway, NJ).

LFA-1 adhesion and clustering assays. Anti-CD3-stimulated or unstimulated T cells were added to plates coated with recombinant murine ICAM-1 (R&D Systems) and incubated for 30 min at 37°C. Nonadherent cells were gently washed off, and the remaining cells were counted as previously described (39). LFA-1 clustering/capping experiments were conducted as follows. Briefly, cells were incubated with 5 µg/ml anti-CD3 (clone 145-2C11) on ice for 30 min. Cells were then washed twice with cold medium and cross-linked by incubation with 10 µg/ml of a rabbit anti-hamster immunoglobulin G antibody (Jackson Immunoresearch) at 37°C for 30 min. Cells were fixed with 2% paraformaldehyde for 20 min, washed with PBS, and blocked with 3% BSA-5% FCS-PBS for 45 min. Samples were then stained for anti-CD11a and an isotype-specific Alexa Fluor 546-conjugated anti-rat antibody as described elsewhere (39). At least 50 T cells were counted for cap formation in each experiment. These experiments were conducted using four different SKAP-55 KO mice. Immunofluorescence microscopy was conducted as described previously (39).

IL-2 ELISA and T-cell proliferation assay. SKAP-55+/+, SKAP-55+/–, and SKAP-55–/– primary mouse cells (2 x 105/well) were cultured in 2 µg/ml anti-CD3 (145-2C11) applied to plates for 48 h. The supernatants were collected, and IL-2 was measured by an enzyme-linked immunosorbent assay (ELISA) with a rat anti-mouse IL-2 monoclonal antibody (ELISA capture) and a biotinylated rat anti-mouse IL-2 monoclonal antibody (ELISA detection). For the T-cell proliferation assay, cells were stimulated with 1 µg/ml plated anti-CD3 for various times (e.g., 48 h). To assess proliferation, cells were pulsed with 1 µCi of [3H]thymidine (Amersham Biosciences) for the last 12 h. Cells were collected on GSC filters (PerkinElmer, Wallace Dy, Finland). Radioactivity was measured in a microplate scintillation counter.

Statistical analysis. Results are given as means ± standard deviations. The difference between means was tested either by an unpaired Student t test (for two means) or by one-way analysis of variance with Dunnett's posttest (multiple test) using GraphPad Prism, version 3.02 for Windows (GraphPad Software, San Diego, CA). A P value of <0.05 was considered significant.


arrow
RESULTS
 
SKAP-55-deficient mice were generated using a gene-trapping approach that allows the identification of sequence tags from the mutated genes. SKAP-55+/– animals were then mated with C57JBL/6 mice to produce 129Sv/lex x C57JBL/6 hybrids. Mice have been backcrossed for more than six generations in this study. Age- and sex-matched mice from the same litters were used in our experiments. PCR was used to genotype SKAP-55 wild-type (SKAP-55+/+), heterozygous (SKAP-55+/–), and KO (SKAP-55–/–) mice. As shown in Fig. 1a and b, upstream primer A, downstream primer B, and primer LTR were designed to amplify the wild-type sequence and the inserted trapping cassette. PCR analysis identified SKAP-55+/+ mice with one wild-type band, SKAP-55–/– mice with one LTR band, and SKAP-55+/– mice with wild-type and LTR bands (Fig. 1b). The loss of SKAP-55 expression in SKAP-55 KO mice was confirmed by full-length SKAP-55 mRNA detection in wild-type and KO mice by reverse transcription-PCR (Fig. 1c) and by anti-SKAP-55 blotting using both C-terminal and N-terminal monoclonal antibodies (Fig. 1d, top panels, lanes 1 and 3 versus lanes 4 and 5). Significantly, SKAP-55-deficient T cells retained ADAP expression at wild-type levels (Fig. 1d, second panel from top, lanes 2 and 3 versus lane 1). Expression of ERK and actin served as positive controls (Fig. 1d). By contrast, and in agreement with a previous report (12), ADAP–/– T cells showed a loss of SKAP-55 expression, as shown by immunoblotting with anti-SKAP-55 (Fig. 1d, bottom panel, lane 6 versus lanes 4 and 5). Our finding that the loss of SKAP-55 does not affect ADAP expression allowed an evaluation of SKAP-55 function in primary cells independent of ADAP expression.


Figure 1
View larger version (26K):
[in this window]
[in a new window]

  		FIG. 1. SKAP-55-deficient mice lack SKAP-55 expression. (a) Site targeted vector used in the generation of SKAP-55–/– mice. SKAP-55 KO mice were generated as described previously (45). (b) PCR analysis of SKAP-55–/– mice. PCR analysis identified SKAP-55+/+ mice with a wild-type (WT) band, SKAP-55–/– mice with an LTR band, and SKAP-55+/– mice with both WT and LTR bands. (c) The loss of SKAP-55 expression in SKAP-55 KO mice was confirmed by full-length SKAP-55 mRNA detection for the WT and KO mice by reverse transcription-PCR. (d) ADAP is expressed in SKAP-55–/– and SKAP-55+/– T cells. Cell lysates were blotted with various antibodies. (Top panels) Anti-SKAP-55. Lanes 1 and 4, WT (SKAP-55+/+); lane 2, heterozygote (SKAP-55+/–); lanes 3 and 5, homozygote (SKAP-55–/–). Anti-N-terminal SKAP-55 antibodies were used in lanes 1 to 3, and anti-C-terminal SKAP-55 antibodies were used in lanes 4 and 5. (Center panels) Anti-ADAP, antiactin, and anti-ERK blots. Lanes 1, WT (SKAP-55+/+); lanes 2, heterozygote (SKAP-55+/–); lanes 3, homozygote (SKAP-55–/–). (Bottom panel) Anti-SKAP-55 blot. Lane 4, WT T cells; lane 5, SKAP-55–/– T cells; lane 6, ADAP–/– T cells. ADAP–/– T cells show a loss of SKAP-55 expression.

We next assessed the responses of SKAP-55-deficient T cells to antigen as presented by APCs (Fig. 2). Purified CD4-positive T cells were cocultured with isolated mature DCs in the presence of the superantigen SEA, followed by assessment of aggregation. Aggregates were first observed at 12 to 24 h, with maximum levels at 45 to 60 h. As seen in Fig. 2a, the addition of SEA induced a concentration-dependent increase in the formation of aggregates at 24 h. Both the number and the size of the aggregates changed with increasing concentrations of SEA from 0.2 to 5 µg/ml (Fig. 2a, upper panels). By contrast, SKAP-55-deficient T cells showed smaller aggregates over the full range of SEA concentrations (Fig. 2a, lower panels). On average, the SKAP-55-deficient aggregates were 5- to 10-fold smaller than those for wild-type T cells. The addition of an anti-LFA-1 antibody prevented aggregate formation (data not shown). Consistent with reduced adhesion, IL-2 production and proliferation were impaired in SKAP-55-deficient T cells (Fig. 2a, upper and lower graphs, respectively). The response was impaired at similar levels in ADAP–/– T cells in terms of cell aggregation (data not shown), IL-2 production (Fig. 2b, upper graph), and proliferation (Fig. 2b, lower graph). These observations provided initial evidence that SKAP-55 is needed for the optimal ability of T cells to form aggregates and to respond to SEA.


Figure 2
View larger version (42K):
[in this window]
[in a new window]

  		FIG. 2. SKAP-55-deficient T cells show defects in SEA-induced T-cell-DC aggregation. (a) Aggregates of SKAP-55-deficient T cells. Freshly isolated SKAP-55+/+ and SKAP-55–/– T cells were exposed to various concentrations of SEA (0 to 5 µg/ml) with DCs and were monitored by light microscopy for aggregation (upper panels), IL-2 production (upper graph), and proliferation (lower graph). (b) Freshly isolated ADAP+/+ and ADAP–/– T cells were exposed to various concentrations of SEA (0 to 5 µg/ml) and monitored for IL-2 production (upper graph) and proliferation (lower graph) at 48 h. Data are means from three replicate wells. Error bars, standard errors of the means. A P value of <0.05 was considered significant.

To extend this, the interaction time of T cells with DCs was measured in the absence and presence of SEA (Fig. 3a). Contact times were monitored for 1,800 s, as outlined in Materials and Methods. SEA induced a shift from short-term to longer-term contacts of SKAP-55+/+ T cells with DCs (Fig. 3a, left histogram), as reported previously (8, 21, 35). By comparison, CD4+ SKAP-55–/– T cells showed much shorter dwell times in the absence of SEA than wild-type cells. Although SEA caused a shift to longer contact times, these dwell times were still more transient than those observed for SKAP-55+/+ T cells. This difference was especially evident when the number of conjugates at >1,800 s was measured (Fig. 3a, right histogram). In the absence of SEA, 60 to 65% of wild-type cells interacted with APCs longer than 1,800 s, while in the presence of SEA, more than 91% of cells formed longer-term contacts. By contrast, only 35% of resting and 45 to 50% of SEA-exposed CD4+ SKAP-55–/– T cells showed longer dwell periods. SKAP-55 therefore influences the duration of contact between T cells and APCs in antigen-dependent and -independent contexts. Further, these shorter contact times paralleled the reduced proliferation and reduced IL-2 production of SKAP-55–/– cells (Fig. 2a).


Figure 3
View larger version (28K):
[in this window]
[in a new window]

  		FIG. 3. SKAP-55-deficient T cells show defects in SEA-induced T-cell-DC conjugation and in the localization of TCR/CD3 at the IS. (a) CD4+ SKAP-55–/– T cells show more-transient interactions between T cells and DCs. Purified CD4+ T cells were incubated with mature DCs that had been preloaded with SEA. (Left) Dot plot analysis of T-cell interactions with DCs. (Right) Histogram showing the percentages of SKAP-55+/+ and SKAP-55–/– T-cell-DC conjugates of >1,800 s. (b) CD4+ SKAP-55–/– T cells show defects in the movement of the TCR/CD3 complex to the IS. Purified CD4+ T cells were incubated with mature DCs that had been preloaded with SEA. The localization of CD3 was imaged after staining with fluorescein isothiocyanate-conjugated anti-CD3. (Left) Histogram showing the percentages of T cells with CD3 localized at the IS. (Top right) Examples of nonlocalized CD3. (Bottom right) Examples of CD3 localized microclusters at the IS. (c) SKAP-55-deficient cells show a general reduction in tyrosine phosphorylation. Wild-type or SKAP-55-deficient splenocytes were incubated with 5 µg/ml SEA for 0, 30, or 60 min at 37°C. Cell lysates were prepared, followed by immunoblotting with antibody 4G10.

Shorter interaction times between T cells and APCs were also reflected in a reduction in the number of cells with localized CD3 at the IS (Fig. 3b). T-cell-DC conjugates that formed after a 15-min exposure to SEA were stained with fluorescein isothiocyanate-conjugated anti-CD3 and examined for the localization of TCR/CD3 at the IS. While the number of cells with CD3 microclusters at the IS increased with the addition of SEA from 8 to 50% (Fig. 3b, left histogram), a lower number of SKAP-55-deficient T cells showed this pattern (i.e., the number increased from 8 to 22%). Examples of conjugate formation with nonlocalized and IS-localized CD3 are shown in the right panels of Fig. 3b. We also observed an overall reduction in the tyrosine phosphorylation of many proteins when splenocytes were incubated with SEA and DCs for either 30 min or 60 min (Fig. 3c). Overall, our observations showed that SKAP-55 can influence the conjugation time, the number of cells with IS-localized CD3, and the proliferation of T cells in response to SEA.

Given the effect on conjugation, it was important next to determine whether loss of SKAP-55 influenced anti-CD3-induced integrin adhesion of primary T cells (Fig. 4). Isolated mesenteric lymph cells were stimulated with anti-CD3, followed by an assessment of fibronectin (FN) and ICAM-1 binding, as previously described (40). First, CD29 (i.e., ß1 FN receptor) expression was the same on SKAP-55+/+ and SKAP-55–/– T cells (Fig. 4a). Despite this, the SKAP-55-deficient cells showed low levels of adhesion to FN relative to those for wild-type cells (Fig. 4b). While anti-CD3 at 2 µg/ml increased wild-type cell adhesion from 4 to 32% of cells, only 15% of SKAP-55–/– T cells bound to FN (Fig. 4b, left). The loss of SKAP-55 therefore resulted in a >50% reduction in the number of cells capable of binding to FN. Examples of adherent cells on plates are shown in the right panels of Fig. 4b.


Figure 4
View larger version (21K):
[in this window]
[in a new window]

  		FIG. 4. Primary SKAP-55–/– T cells show impaired FN adhesion. Cells were stimulated with anti-CD3 (2C11), and binding to immobilized FN on plates was measured as described in Materials and Methods. (a) Fluorescence-activated cell sorter profile of CD29 surface staining on SKAP-55+/+ and SKAP-55–/– T cells. FITC, fluorescein isothiocyanate. (b) (Left) Histogram showing the levels of binding of SKAP-55+/+ and SKAP-55–/– T cells. Data are means ± standard errors of the means for three replicate wells. A P value of <0.05 was considered significant. (Right) Light microscopy images of cells on plates.

Defective adhesion was also noted in binding to immobilized ICAM-1 on plates (Fig. 5). CD18 (i.e., LFA-1 ß2 chain) expression was the same on SKAP-55+/+ and SKAP-55–/– T cells (Fig. 5a). While anti-CD3 increased the level of adhesion of wild-type T cells to ICAM-1 from 2 to 30% of cells, only 19% of SKAP-55–/– T cells bound to plates (Fig. 5a, center). Images of adherent cells are shown in the right panels of Fig. 5a. Comparison of SKAP-55- and ADAP-deficient primary T cells showed similar levels of impaired binding to ICAM-1 (Fig. 5b). Occasionally, ADAP–/– cells showed a slightly greater impairment; however, this was not a reproducible finding. Last, the impaired adhesion of SKAP-55–/– T cells was reflected in a reduction of LFA-1 clustering on the surfaces of cells (Fig. 5c). For this experiment, freshly isolated lymph node cells were stimulated with anti-CD3 for 30 min, followed by staining with anti-CD11a, as previously described (39). While anti-CD3 induced an increase in the numbers of SKAP-55+/– cells with clustered LFA-1 (i.e., from 15 to 40%), fewer SKAP-55–/– cells showed LFA-1 clustering (i.e., an increase from 8 to 18%) (Fig. 5c, left). Examples of the LFA-1 clustered cells are shown in the right panels of Fig. 5c. Overall, these data show that the reduction in the ability of a significant portion of SKAP-55–/– T cells to bind to ICAM-1 was accompanied by a defect in LFA-1 clustering on the surface of T cells.


Figure 5
View larger version (21K):
[in this window]
[in a new window]

  		FIG. 5. Primary SKAP-55–/– T cells have impaired ICAM-1 adhesion and LFA-1 clustering. (a) Reduced ICAM-1 adhesion of SKAP-55-deficient T cells. (Left) Fluorescence-activated cell sorter profile showing CD18 surface expression on SKAP-55+/+ and SKAP-55–/– T cells. FITC, fluorescein isothiocyanate. (Center) Histogram showing difference in the induction of ICAM-1 adhesion. (Right) Light microscopy images of SKAP-55+/+ and SKAP-55–/– T cells. (b) Comparison of defective ICAM-1 binding between ADAP–/– and SKAP-55–/– T cells. Cells were stimulated with anti-CD3 (2C11), followed by measurement of cell binding to immobilized ICAM-1 on plates. Data are means ± standard errors of the means for three replicate wells. WT, wild type. (c) SKAP-55-deficient T cells have impaired LFA-1 clustering. Cells were incubated with anti-CD3 (2C11) and a rabbit anti-hamster secondary antibody, and LFA-1 surface expression was visualized as described previously (39). (Left) Histogram with the mean percentages (± standard deviations) of T cells that have formed polarized LFA-1 caps. At least 50 cells were counted for each sample by using fluorescence microscopy. (Right) Examples of LFA-1 clustering for SKAP-55+/– and SKAP-55–/– cells.

This defect in adhesion was reflected in reductions in cytokine production and proliferation by SKAP-55–/– T cells (Fig. 6). Anti-CD3 was used to activate T cells, followed by assessments of IL-2 and IFN-{gamma} production. While anti-CD3 increased IL-2 production from 70 to 630 pg/ml in SKAP-55+/+ cells at 48 h of culture, IL-2 production at this time was only 350 pg/ml for SKAP-55+/– and 280 pg/ml for SKAP-55–/– T cells (Fig. 6a). Similar decreases were observed at other time points (i.e., 36 and 60 h) (data not shown). IFN-{gamma} production was also reduced in SKAP-55-deficient T cells (Fig. 6b). Intracellular staining was used to measure IFN-{gamma} as described in Materials and Methods. On average, there was a 40 to 50% reduction in the percentage of T cells expressing IFN-{gamma}. This reduction in adhesion and cytokine production was mirrored by reduced SKAP-55–/– T-cell proliferation (Fig. 6c). Proliferation was 50 to 75% lower for SKAP-55–/– T cells than for wild-type T cells. The reduced proliferation of SKAP-55+/– and SKAP-55–/– cells closely resembled that of ADAP+/– and ADAP–/– T cells. These observations indicate that SKAP-55 expression in primary cells is needed for optimal IL-2 and IFN-{gamma} production and proliferation.


Figure 6
View larger version (34K):
[in this window]
[in a new window]

  		FIG. 6. SKAP-55-deficient T cells show defects in cytokine production and proliferation. Cells were stimulated with anti-CD3 (2C11), followed by measurement of IL-2 and IFN-{gamma} production. (a) SKAP-55–/– T cells showed lower IL-2 production levels than SKAP-55+/+ T cells. SKAP-55+/– IL-2 production was 50% lower, and SKAP-55–/– IL-2-production was 60 to 70% lower. (b) SKAP-55–/– T cells showed reduced IFN-{gamma} production. (c) SKAP-55-and ADAP-deficient T cells showed similar impairment of proliferation. Cells were stimulated with anti-CD3, followed by [3H]thymidine incorporation. Data are means from three replicate wells. Error bars, standard errors of the means. A P value of <0.05 was considered significant.

The partial reduction in responses was unexpected given previous reports of a more complete blockade in cells lacking the ADAP-SKAP-55 module (10, 27). The reduction in cytokine production by, and proliferation of, SKAP-55-deficient T cells could be related to a general impairment of function in the overall population or could reflect the presence of subsets of SKAP-55-dependent and -independent T cells. The binding of a portion of SKAP-55–/– T cells to ICAM-1 plates (Fig. 5) and their ability to form conjugates with TCR/CD3 localization at the IS in response to SEA (Fig. 3) supported the notion of a subset of SKAP-55-independent cells. To assess this in more depth in the context of proliferation, T cells were labeled with CSFE and monitored for the number of cell divisions in response to anti-CD3 (2 µg/ml) by using flow cytometry (Fig. 7A). While resting SKAP-55+/+ T cells showed a single peak (Fig. 7a, top left), anti-CD3 induced several cell divisions from M2 to M4 (top right). A decreasing number of wild-type cells was observed in each successive division: 35% in M2, 15% in M3, and 2.6% in M4 (Fig. 7a). SKAP-55-deficient T cells differed from wild-type cells in that fewer cells entered the first round of division (Fig. 7a, bottom panels). In this case, 69% of cells remained in M1 compared to 46% of SKAP-55+/+ cells. The SKAP-55–/– T cells that entered the first round of division continued to M3 and M4 with the same decreasing frequency as the SKAP-55+/+ cells (Fig. 7b). Overall, these data indicate that the peripheral T-cell compartment is heterogeneous, with some T cells requiring and other cells not requiring SKAP-55 to undergo cell division. The principal defect in the SKAP-55–/– response was an inability of a subset of T cells to enter the first phase of the cell cycle. Once committed, the other proliferating subset of SKAP-55–/– cells cycled in the same way as wild-type cells.


Figure 7
View larger version (36K):
[in this window]
[in a new window]

  		FIG. 7. Identification of SKAP- and ADAP-dependent and -independent T-cell subsets. Dependency differs with the strength of the TCR signal. (a) Subsets of primary SKAP-55–/– T cells were dependent on or independent of SKAP-55 for entry into the cell cycle as detected by CFSE labeling. Freshly isolated T cells were labeled with CFSE and either left in a resting state or stimulated with anti-CD3. Then they were monitored for cell division by fluorescence-activated cell sorting. (Top) Wild-type SKAP-55+/+ cells. (Left) Resting cells; (right) anti-CD3-activated cells. (Bottom) SKAP-55–/– cells. (Left) Resting cells; (right) anti-CD3-activated cells. (b) Percentages of cells undergoing cell division (M2, first division; M3, second division; M4, third division). A decreasing number of cells undergo multiple divisions. The decreases in the abilities of activated SKAP-55+/+ and SKAP-55–/– T cells to undergo subsequent divisions are similar. (c) The requirements for SKAP-55 and ADAP for ICAM-1 adhesion differ with the potency of the TCR signal. T cells were stimulated with anti-CD3 (2C11), and the binding of cells to ICAM-1 immobilized on plates was then measured. (Left) SKAP-55+/+ and SKAP-5–/– T cells; (right) ADAP+/+ and ADAP–/– T cells. (d) The requirements for SKAP-55 and ADAP for proliferation differ with the potency of the TCR signal. Cells were stimulated with anti-CD3, followed by [3H]thymidine incorporation at 60 h. (Left) SKAP-55+/+, SKAP-55+/–, and SKAP-55–/– T cells; (right) ADAP+/+, ADAP+/–, and ADAP–/– T cells. Data are means from three replicate wells. Error bars, standard errors of the means.

Given the presence of these two populations, it was of interest next to assess whether the dependency on SKAP-55 could be influenced by the strength of the TCR/CD3 signal. For this purpose, anti-CD3 antibody concentrations were varied from 1 to 10 µg/ml. Our studies so far had used 2 µg/ml of anti-CD3 to activate cells. As seen in Fig. 7c (left), 2 µg/ml of anti-CD3 increased the numbers of SKAP-55+/+ and SKAP-55–/– T cells binding to ICAM-1 relative to those exposed to 1 µg/ml (from 105 to 123 wild-type cells and from 60 to 90 KO cells). The use of 10 µg/ml (or 5 µg/ml) essentially induced similar levels of binding to ICAM-1 (160 cells in both cases). This indicated that an increase in the concentration of the antibody (i.e., the strength of the TCR/CD3 signal) can override the dependency of T cells on SKAP-55. A comparable situation was observed for ADAP–/– T cells: dependency on ADAP was reduced with increasing levels of anti-CD3 antibody (Fig. 7c, right). Again, the use of 10 µg/ml obviated the need for ADAP expression in the induction of ICAM-1 adhesion. These results were matched by those for the proliferation of T cells, where the use of 10 µg/ml increased the similar levels of proliferation of SKAP-55+/+ versus SKAP-55–/– and ADAP+/+ versus ADAP–/– T cells (Fig. 7d). Therefore, while these concentrations of anti-CD3 and the potency of the resultant signal are likely to be much greater than those that would be encountered in response to a foreign peptide, our observations make the important point that the dependency on SKAP-55 and ADAP is relative and differs with the potency of the TCR signal. They might also indicate that TCR signaling can use an alternate pathway in the regulation of adhesion.

We next assessed the numbers of cells in the thymi of SKAP-55+/+, SKAP-55+/–, and SKAP-55–/– mice (Fig. 8a). Although an occasional SKAP-55–/– mouse showed a reduction in thymic cellularity, this was not found to be statistically significant over five independent experiments. The expression of the antigen receptor (TCR) was normal for SKAP-55+/– and SKAP-55–/– thymocytes (Fig. 8b), as was the presence of CD4 and CD8 double-negative (DN), double-positive (DP), and CD4 or CD8 single-positive (SP) cells (Fig. 8c). Using CD44 and CD25 as markers, the DN1-to-DN4 transition appeared normal in SKAP-55+/– and SKAP-55–/– mice (Fig. 8d). A slight reduction in the CD44- and CD25-negative compartment was occasionally observed; but this was not a consistent observation. We also confirmed that normal ratios of T and B cells were observed in the lymph nodes and spleen when anti-CD3 and anti-B220 were used to stain cells (Fig. 9a), and normal ratios of peripheral mature CD4 SP and CD8 SP T-cell subsets were observed (Fig. 9b). Occasionally, SKAP-55–/– and SKAP-55+/– mice showed slight increases in the numbers of CD4+ CD8+ T cells; however, this was not reproducible. In addition, the compartments of {gamma}/{delta} T cells in the lymph nodes and spleen were equivalent in SKAP-55+/+, SKAP-55+/–, and SKAP-55–/– mice (Fig. 9c), as were those of NK cells as defined by CD3– DX5+ staining (Fig. 9d). These data confirm a normal composition of cells in the peripheral T-cell compartments of SKAP-55-deficient mice.


Figure 8
View larger version (48K):
[in this window]
[in a new window]

  		FIG. 8. SKAP-55-deficient mice show normal thymic cellularity and normal stages of thymic differentiation. (a) Normal numbers of thymocytes in SKAP-55–/– mice. (b) Anti-TCR staining of thymocytes of SKAP-55+/+, SKAP-55+/–, and SKAP-55–/– mice. TCR expression on thymocytes from SKAP-55–/– and SKAP-55+/– mice was normal. FITC, fluorescein isothiocyanate. (c) Anti-CD4 and anti-CD8 staining of thymocytes of SKAP-55+/+, SKAP-55+/–, and SKAP-55–/– mice. Normal numbers of CD4 or CD8 SP, DP, and DN thymocytes were observed in SKAP-55–/– and SKAP-55+/– mice. (d) Anti-CD44 and anti-CD25 staining of thymocytes from SKAP-55+/+, SKAP-55+/–, and SKAP-55–/– mice. Thymic development involves the progression from stage DN1 (CD25– CD44+) to DN2 (CD25+ CD44+), DN3 (CD25+ CD44–), and DN4 (CD25– CD44–). Five independent experiments were conducted, and representative results from one set of experiments are shown.


Figure 9
View larger version (48K):
[in this window]
[in a new window]

  		FIG. 9. SKAP-55-deficient mice show normal composition of T-cell and B-cell subsets and NK cells. Cells from the lymph nodes (LN) and spleens of SKAP-55+/+, SKAP-55+/–, and SKAP-55–/– mice were stained with either anti-CD3 versus anti-B220 (a), anti-CD4 versus anti-CD8 (b), anti-CD3 versus anti-TCR{gamma}{delta} (c), or anti-CD3 versus anti-DX5 (d). Ctl, control. (a) Normal levels of T and B cells were observed in SKAP-55–/– LN (top) and splenocytes (bottom). (b) Normal levels of CD4 SP and CD8 SP T cells were observed in SKAP-55–/– LN (top) and splenocytes (bottom). (c) Normal levels of {gamma}/{delta} T cells were observed in SKAP-55–/– and SKAP-55–/+ LN (top) and splenocytes (bottom). (d) Normal levels of one subset of NK cells (CD3– DX5+) were observed in SKAP-55–/– and SKAP-55–/+ LN (top) and splenocytes (bottom). Four independent experiments were conducted, and representative results from one set of experiments are shown.


arrow
DISCUSSION
 
Although current data support a central role for the ADAP-SKAP-55 module in the regulation of TCR-induced integrin clustering and adhesion (9, 10, 12, 13, 14, 18, 27), it has been unclear whether ADAP and/or SKAP-55 acts as an effector of the response. Since ADAP controls SKAP-55 degradation and ADAP-deficient T cells lack SKAP-55 expression (i.e., ADAP SKAP-55 double deficient), it had not been possible to determine whether the immunodeficient phenotype was due to ADAP and/or to SKAP-55. In this study, we have reported the phenotype of the SKAP-55-deficient mouse where SKAP-55–/– T cells retain ADAP expression yet show defects in integrin clustering and adhesion, IL-2 and IFN-{gamma} cytokine production, and proliferation. In fact, SKAP-55–/– T cells showed the same general impairment of function as ADAP–/– T cells, indicating that SKAP-55 is an effector of the ADAP-SKAP-55 module. At the same time, the requirement for ADAP and SKAP-55 was not absolute, since a subset of peripheral T cells adhere without the need for either adaptor. Further, dependency on SKAP-55 or ADAP differed with the strength of the TCR signal. Higher concentrations of anti-CD3 enabled greater numbers of cells to bind ICAM-1 and proliferate in the absence of SKAP-55 or ADAP. Our findings identify a clear effector role for SKAP-55 in the up-regulation of LFA-1 adhesion in peripheral T cells and demonstrate that higher levels of TCR engagement can bypass the need for SKAP-55 and ADAP in the up-regulation of T-cell adhesion.

ADAP and SKAP-55 form a module where they bind to each other with high stoichiometry, and immunoprecipitation with an anti-ADAP antibody depletes most SKAP-55 from cell lysates and vice versa (4, 25). Further, ADAP controls the degradation of SKAP-55 such that ADAP-deficient T cells have little if any SKAP-55 protein expression (12) (Fig. 1d). The question of whether SKAP-55 might also influence ADAP expression was addressed previously; small interfering RNA-mediated reduction of SKAP-55 levels in a mouse antigen-specific T-cell line markedly reduced integrin adhesion without altering ADAP expression (13). Similar findings have recently been reported with Jurkat cells (18). Our study has now extended this finding by demonstrating that SKAP-55 has no obvious role in regulating ADAP expression in normal primary peripheral T cells. ADAP therefore serves a unidirectional chaperone function for its binding partner within the module.

This observation, combined with the finding of impaired ß1- and ß2-mediated adhesion of SKAP-55–/– T cells, has, for the first time, identified an effector role for SKAP-55 within the ADAP-SKAP-55 module in primary T cells. SKAP-55-deficient T cells showed multiple defects, including defects in cell aggregation and subsequent cytokine production and proliferation in response to SEA (Fig. 2) and defects in anti-CD3-induced ICAM-1 adhesion, cytokine production, and proliferation (Fig. 6). Occasionally, ADAP–/– cells showed greater impairment of ICAM-1 binding; however, this was not a consistent observation. Instead, the level of defective function for SKAP-55-deficient T cells was remarkably similar to that for ADAP-deficient T cells. This was observed in multiple assays including assessment of cell aggregation, cytokine production, and proliferation in response to SEA (Fig. 2), ICAM-1 binding (Fig. 5b), anti-CD3-induced proliferation (Fig. 6c), and the abilities of high anti-CD3 concentrations to bypass the need for the adaptor (Fig. 7c and d). These findings introduce the intriguing question of whether the impaired function of ADAP-deficient mice is due primarily to the loss of SKAP-55 or whether ADAP can play an additional, independent role in adhesion. In the former scenario, SKAP-55 would be the sole effector of the ADAP-SKAP-55 module. ADAP would indirectly influence adhesion by altering SKAP-55 expression, or possibly its conformation or localization. In the more likely scenario, ADAP provides overlapping or alternate signals for adhesion. The presence of an EVH1 domain and G-actin binding motifs in ADAP would support this alternate role (19). The modulation of the cytoskeleton at the IS could facilitate more effective adhesion without directly affecting the status of LFA-1 on the surface of the cell. However, it is interesting that multiple mutations in the EVH1 motif had no obvious effect on the ability of T cells to form conjugates with APCs (39). In either case, ADAP acts to bridge SLP-76 with downstream SKAP-55. p59fynT phosphorylates YDDV sites in ADAP, leading to SLP-76 SH2 domain binding and possibly to recruitment of the ADAP-SLP-76-GADS complex to LAT (31, 32, 41). Mutation of YDDV sites in ADAP impairs conjugation, SMAC formation, and cytokine production (39). Further, loss of the SKAP-55 SH3 domain, loss of the ADAP SH3c domain, or over expression of a peptide which binds to the proline-rich region of ADAP ablates adhesion (7, 18, 39). These observations are consistent with the existence of a cascade linking SLP-76 to ADAP and ADAP to SKAP-55 in the control of adhesion. The data from this study now underscore the central role of SKAP-55 as a downstream effector in the cascade.

The loss of SKAP-55 impaired anti-CD3-induced adhesion to FN (Fig. 4) and ICAM-1 (Fig. 5). Generally ß1-mediated binding was impaired more than ß2-mediated binding, introducing the possibility that SKAP-55 may differentially regulate the different classes of integrins. In terms of response to antigens, the loss of SKAP-55 reduced SEA-induced T-cell aggregation (Fig. 2) and the dwell times of T cells with APCs (Fig. 3). The length of time that a T-cell spends attached to a DC will influence the level of antigen-receptor clustering and the intracellular signaling needed for proliferation. In this regard, while the difference in general cell aggregation induced by SEA did not correlate strictly with reduced proliferation, a correspondence between the reduction in the percentage of long-term contacts (i.e., >1,800 s), the reduction in the number of cells with the TCR/CD3 complex localized at the IS, and the level of IL-2 production and proliferation was observed (i.e., 40 to 50%) (Fig. 2 and 3). A small reduction in number of TCR/CD3 microclusters in SKAP-55–/– T cells was also observed (data not shown). TCR/CD3 and intracellular signaling proteins form microclusters at the IS, where signals are generated for proliferation (5, 8, 35). The reduction in conjugation time and TCR microcluster formation in turn correlated with an overall reduction in the tyrosine phosphorylation of many proteins in T cells (Fig. 3c). To date, a selective reduction in the phosphorylation of one protein relative to another has not been observed.

Interestingly, SKAP-55 also played a role in antigen-independent conjugate formation. Even in the absence of SEA, SKAP-55–/– T cells interacted with APCs for shorter periods than wild-type cells (Fig. 3a). The addition of SEA caused a shift to longer adhesion periods for both SKAP-55+/+ and SKAP-55–/– cells. The inability of SKAP-55–/– cells to achieve longer contact times appeared to be influenced by the fact that they already exhibited shorter contacts in an antigen-independent context. Only 40% of SKAP-55–/– T cells achieved the longer conjugation periods (i.e., >1,800 s) in response to SEA. Many SKAP-55–/–cells failed to respond to TCR ligation, while others formed conjugates of intermediate duration. Overall, our findings are consistent with a general role for SKAP-55 in antigen-dependent and antigen-independent conjugate formation.

One surprising observation was the existence of heterogeneity in the peripheral T-cell population with regard to their dependence on SKAP-55 and ADAP. A percentage of SKAP-55–/– T cells underwent normal adhesion, conjugation, and proliferation (Fig. 2 to 6). In fact, some 30 to 50% of SKAP-55- and ADAP-deficient T cells proliferated normally in response to SEA presented by DCs (Fig. 2), bound to ICAM-1 on plates (Fig. 5), produced IL-2 and IFN-{gamma}, and proliferated (Fig. 6). Cell cycle analysis extended this observation by showing that a significant portion of SKAP-55-deficient T cells underwent multiple rounds of cell division (Fig. 7). Once committed to proliferation, these cells underwent the same number of subsequent cell divisions as wild-type cells. The defect was in the ability of a subset of T cells to enter the first cycle of division. This indicates that the "inside-out" pathway is more complex than previously appreciated and that SKAP-55 is not essential for the function of all peripheral T cells. Similar findings were made with ADAP-deficient T cells. A subset of T cells appears to have an alternate mechanism that can substitute for SKAP-55.

Further, it is noteworthy that the relative proportions of SKAP-55- and ADAP-dependent and -independent cells in the peripheral compartment are not fixed. Rather, they appear to reflect a heterogeneity in the population of T cells that can be altered by the strength of the TCR signal. An increase in the anti-CD3 concentration from 1 to 2 µg/ml (otherwise, 2 µg/ml was used throughout the study) and 10 µg/ml induced increasing numbers of cells to adhere to ICAM-1 and to proliferate without the need for SKAP-55 or ADAP (Fig. 7c and d). In fact, 10 µg of antibody/ml activated the entire population of T cells without the need for SKAP-55 or ADAP. This level of receptor cross-linking and this signal strength are probably higher than those that would normally be encountered under physiological conditions. Nevertheless, the observation is informative in that it indicates that SKAP-55 and ADAP are not obligatory components in the TCR signaling leading to adhesion. The strength of the signal can override the need for SKAP-55. In this context, the ADAP/SKAP-55 pathway may preferentially support low- to intermediate-affinity ligand stimulation of T cells. The degree to which the ADAP/SKAP-55 pathway supports physiological responses to peptides of different affinities and the nature of the alternate pathway remain to be determined. Another protein(s) may constitute a pathway that is distinct from SKAP-55, or the TCR complex may directly activate another mediator downstream of SKAP-55 in the same pathway. Possible candidates include the GTPase Rap1 and/or its binding partners RapL and RIAM (6, 15, 16, 22, 28, 34).

Last, a similarity between the phenotypes of the SKAP-55- and ADAP-deficient mice was also observed in the lack of a profound effect on thymic differentiation and the composition of the peripheral T-cell compartment. Both ADAP- and SKAP-55-deficient mice expressed a normal array of thymocytes (i.e., CD4, CD8, DN1 to DN4) and peripheral cell subsets (i.e., CD4 SP, CD8 SP, and TCR{gamma}{delta} T cells; B-cells; and NK cells) (Fig. 8 and 9). These findings do not exclude the possibility that more-subtle alterations will eventually be observed in TCR-transgenic animals, as reported for ADAP–/– mice (42). We occasionally observed a decrease in SKAP-55–/– thymic cellularity; however, when averaged over six animals, this difference was not found to be statistically significant. This differs from the observations for ADAP–/– mice, which showed a moderate decrease in thymocyte numbers (10, 27, 43). In instances of mice with decreased numbers of thymocytes, the cells showed an increased propensity to undergo cell death in media (data not shown). Although occasionally SKAP-55–/– thymocytes showed slight increases in the percentages of DN2 and DN3 thymocytes with a reduction in the percentage of the DN4 subset, this also was not reproducibly different from findings for non-TCR-transgenic mice. Consistent with this, LFA-1-deficient mice show no apparent defects in thymic differentiation (36). Further studies will be needed to uncover whether ADAP- and SKAP-55-deficient T cells influence the activation of other pathways linked to the regulation of T-cell adhesion.


arrow
ACKNOWLEDGMENTS
 
This work was supported from a grant from the Wellcome Trust, London, United Kingdom (C.E.R. is the recipient of a Principal Research Fellow Award).


arrow
FOOTNOTES
 
* Corresponding author. Mailing address: Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, United Kingdom. Phone: 44 1223 765 661. Fax: 44 1223 764 733. E-mail: cer51{at}cam.ac.uk Back

{triangledown} Published ahead of print on 23 July 2007. Back


arrow
REFERENCES
 
    1
  1. Barber, E. K., J. D. Dasgupta, S. F. Schlossman, J. M. Trevillyan, and C. E. Rudd. 1989. The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex. Proc. Natl. Acad. Sci. USA 86:3277-3281.[Abstract/Free Full Text]
    2. 2
  2. Cantrell, D. A. 2003. GTPases and T cell activation. Immunol. Rev. 192:122-130.[CrossRef][Medline]
    3. 3
  3. da Silva, A. J., Z. Li, C. de Vera, E. Canto, P. Findell, and C. E. Rudd. 1997. Cloning of a novel T-cell protein FYB binds FYN and SH2-domain containing leukocyte protein 76 and modulates interleukin 2 production. Proc. Natl. Acad. Sci. USA 94:7493-7498.[Abstract/Free Full Text]
    4. 4
  4. da Silva, A. J., J. M. Rosenfield, I. Mueller, A. Bouton, H. Hirai, and C. E. Rudd. 1997. Biochemical analysis of p120/130: a protein-tyrosine kinase substrate restricted to T and myeloid cells. J. Immunol. 158:2007-2016.[Abstract]
    5. 5
  5. Davis, D. M., and M. L. Dustin. 2004. What is the importance of the immunological synapse? Trends Immunol. 25:323-327.[CrossRef][Medline]
    6. 6
  6. de Bruyn, K. M., S. Rangarajan, K. A. Reedquist, C. G. Figdor, and J. L. Bos. 2002. The small GTPase Rap1 is required for Mn2+- and antibody-induced LFA-1- and VLA-4-mediated cell adhesion. J. Biol. Chem. 277:29468-29476.[Abstract/Free Full Text]
    7. 7
  7. Duke-Cohan, J. S., H. Kang, H. Liu, and C. E. Rudd. 2006. Regulation and function of SKAP-55 non-canonical motif binding to the SH3c domain of adhesion and degranulation-promoting adaptor protein. J. Biol. Chem. 281:13743-13750.[Abstract/Free Full Text]
    8. 8
  8. Dustin, M. L., T. G. Bivona, and M. R. Philips. 2004. Membranes as messengers in T cell adhesion signaling. Nat. Immunol. 5:363-372.[CrossRef][Medline]
    9. 9
  9. Geng, L., S. Pfister, S. K. Kraeft, and C. E. Rudd. 2001. Adaptor FYB (Fyn-binding protein) regulates integrin-mediated adhesion and mediator release: differential involvement of the FYB SH3 domain. Proc. Natl. Acad. Sci. USA 98:11527-11532.[Abstract/Free Full Text]
    10. 10
  10. Griffiths, E. K., C. Krawczyk, Y. Y. Kong, M. Raab, S. J. Hyduk, D. Bouchard, V. S. Chan, I. Kozieradzki, A. J. Oliveira-Dos-Santos, A. Wakeham, P. S. Ohashi, M. I. Cybulsky, C. E. Rudd, and J. M. Penninger. 2001. Positive regulation of T cell activation and integrin adhesion by the adapter Fyb/Slap. Science 293:2260-2263.[Abstract/Free Full Text]
    11. 11
  11. Hogg, N., M. Laschinger, K. Giles, and A. McDowall. 2003. T-cell integrins: more than just sticking points. J. Cell Sci. 116:4695-4705.[Abstract/Free Full Text]
    12. 12
  12. Huang, Y., D. D. Norton, P. Precht, J. L. Martindale, J. K. Burkhardt, and R. L. Wange. 2005. Deficiency of ADAP/Fyb/SLAP-130 destabilizes SKAP55 in Jurkat T cells. J. Biol. Chem. 280:23576-23583.[Abstract/Free Full Text]
    13. 13
  13. Jo, E. K., H. Wang, and C. E. Rudd. 2005. An essential role for SKAP-55 in LFA-1 clustering on T cells that cannot be substituted by SKAP-55R. J. Exp. Med. 201:1733-1739.[Abstract/Free Full Text]
    14. 14
  14. Kang, H., C. Freund, J. S. Duke-Cohan, A. Musacchio, G. Wagner, and C. E. Rudd. 2000. SH3 domain recognition of a proline-independent tyrosine-based RKxxYxxY motif in immune cell adaptor SKAP55. EMBO J. 19:2889-2899.[CrossRef][Medline]
    15. 15
  15. Katagiri, K., M. Hattori, N. Minato, and T. Kinashi. 2002. Rap1 functions as a key regulator of T-cell and antigen-presenting cell interactions and modulates T-cell responses. Mol. Cell. Biol. 22:1001-1015.[Abstract/Free Full Text]
    16. 16
  16. Katagiri, K., A. Maeda, M. Shimonaka, and T. Kinashi. 2003. RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. Nat. Immunol. 4:741-748.[CrossRef][Medline]
    17. 17
  17. Kinbara, K., L. E. Goldfinger, M. Hansen, F. L. Chou, and M. H. Ginsberg. 2003. Ras GTPases: integrins' friends or foes? Nat. Rev. Mol. Cell Biol. 4:767-776.[Medline]
    18. 18
  18. Kliche, S., D. Breitling, M. Togni, R. Pusch, K. Heuer, X. Wang, C. Freund, A. Kasirer-Friede, G. Menasche, G. A. Koretzky, and B. Schraven. 2006. The ADAP/SKAP55 signaling module regulates T-cell receptor-mediated integrin activation through plasma membrane targeting of Rap1. Mol. Cell. Biol. 26:7130-7144.[Abstract/Free Full Text]
    19. 19
  19. Krause, M., A. S. Sechi, M. Konradt, D. Monner, F. B. Gertler, and J. Wehland. 2000. Fyn-binding protein (Fyb)/SLP-76-associated protein (SLAP), Ena/vasodilator-stimulated phosphoprotein (VASP) proteins and the Arp2/3 complex link T cell receptor (TCR) signaling to the actin cytoskeleton. J. Cell Biol. 149:181-194.[Abstract/Free Full Text]
    20. 20
  20. Krawczyk, C., A. Oliveira-dos-Santos, T. Sasaki, E. Griffiths, P. S. Ohashi, S. Snapper, F. Alt, and J. M. Penninger. 2002. Vav1 controls integrin clustering and MHC/peptide-specific cell adhesion to antigen-presenting cells. Immunity 16:331-343.[CrossRef][Medline]
    21. 21
  21. Kupfer, A., and H. Kupfer. 2003. Imaging immune cell interactions and functions: SMACs and the immunological synapse. Semin. Immunol. 15:295-300.[CrossRef][Medline]
    22. 22
  22. Lafuente, E. M., A. A. van Puijenbroek, M. Krause, C. V. Carman, G. J. Freeman, A. Berezovskaya, E. Constantine, T. A. Springer, F. B. Gertler, and V. A. Boussiotis. 2004. RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion. Dev. Cell 7:585-595.[CrossRef][Medline]
    23. 23
  23. Liu, J., H. Kang, M. Raab, A. J. da Silva, S. K. Kraeft, and C. E. Rudd. 1998. FYB (FYN binding protein) serves as a binding partner for lymphoid protein and FYN kinase substrate SKAP55 and a SKAP55-related protein in T cells. Proc. Natl. Acad. Sci. USA 95:8779-8784.[Abstract/Free Full Text]
    24. 24
  24. Marie-Cardine, A., E. Bruyns, C. Eckerskorn, H. Kirchgessner, S. C. Meuer, and B. Schraven. 1997. Molecular cloning of SKAP55, a novel protein that associates with the protein tyrosine kinase p59fyn in human T-lymphocytes. J. Biol. Chem. 272:16077-16080.[Abstract/Free Full Text]
    25. 25
  25. Marie-Cardine, A., L. R. Hendricks-Taylor, N. J. Boerth, H. Zhao, B. Schraven, and G. A. Koretzky. 1998. Molecular interaction between the Fyn-associated protein SKAP55 and the SLP-76-associated phosphoprotein SLAP-130. J. Biol. Chem. 273:25789-25795.[Abstract/Free Full Text]
    26. 26
  26. Musci, M. A., L. R. Hendricks-Taylor, D. G. Motto, M. Paskind, J. Kamens, C. W. Turck, and G. A. Koretzky. 1997. Molecular cloning of SLAP-130, an SLP-76-associated substrate of the T cell antigen receptor-stimulated protein tyrosine kinases. J. Biol. Chem. 272:11674-11677.[Abstract/Free Full Text]
    27. 27
  27. Peterson, E. J., M. L. Woods, S. A. Dmowski, G. Derimanov, M. S. Jordan, J. N. Wu, P. S. Myung, Q. H. Liu, J. T. Pribila, B. D. Freedman, Y. Shimizu, and G. A. Koretzky. 2001. Coupling of the TCR to integrin activation by Slap-130/Fyb. Science 293:2263-2265.[Abstract/Free Full Text]
    28. 28
  28. Price, L. S., and J. L. Bos. 2004. RAPL: taking the Rap in immunity. Nat. Immunol. 5:1007-1008.[CrossRef][Medline]
    29. 29
  29. Raab, M., A. J. da Silva, P. R. Findell, and C. E. Rudd. 1997. Regulation of Vav-SLP-76 binding by ZAP-70 and its relevance to TCR{zeta}/CD3 induction of interleukin-2. Immunity 6:155-164.[CrossRef][Medline]
    30. 30
  30. Raab, M., H. Kang, A. da Silva, X. Zhu, and C. E. Rudd. 1999. FYN-T-FYB-SLP-76 interactions define a T-cell receptor {zeta}/CD3-mediated tyrosine phosphorylation pathway that up-regulates interleukin 2 transcription in T-cells. J. Biol. Chem. 274:21170-21179.[Abstract/Free Full Text]
    31. 31
  31. Rudd, C. E. 1999. Adaptors and molecular scaffolds in immune cell signaling. Cell 96:5-8.[CrossRef][Medline]
    32. 32
  32. Samelson, L. 2002. Signal transduction mediated by the T cell antigen receptor: the role of adapter proteins. Annu. Rev. Immunol. 20:371-394.[CrossRef][Medline]
    33. 33
  33. Schwartzberg, P. L., L. D. Finkelstein, and J. A. Readinger. 2005. TEC-family kinases: regulators of T-helper-cell differentiation. Nat. Rev. Immunol. 5:284-295.[CrossRef][Medline]
    34. 34
  34. Sebzda, E., M. Bracke, T. Tugal, N. Hogg, and D. A. Cantrell. 2002. Rap1A positively regulates T cells via integrin activation rather than inhibiting lymphocyte signaling. Nat. Immunol. 3:251-258.[CrossRef][Medline]
    35. 35
  35. Shaw, A. S. 2001. FERMing up the synapse. Immunity 15:683-686.[CrossRef][Medline]
    36. 36
  36. Shier, P., G. Otulakowski, K. Ngo, J. Panakos, E. Chourmouzis, L. Christjansen, C. Y. Lau, and W. P. Fung-Leung. 1996. Impaired immune responses toward alloantigens and tumor cells but normal thymic selection in mice deficient in the ß2 integrin leukocyte function-associated antigen-1. J. Immunol. 157:5375-5386.[Abstract]
    37. 37
  37. Takagi, J., B. M. Petre, T. Walz, and T. A. Springer. 2002. Global conformational rearrangements in integrin extracellular domains in outside-in and inside-out signaling. Cell 110:599-611.[CrossRef][Medline]
    38. 38
  38. Togni, M., K. D. Swanson, S. Reimann, S. Kliche, A. C. Pearce, L. Simeoni, D. Reinhold, J. Wienands, B. G. Neel, B. Schraven, and A. Gerber. 2005. Regulation of in vitro and in vivo immune functions by the cytosolic adaptor protein SKAP-HOM. Mol. Cell. Biol. 25:8052-8063.[Abstract/Free Full Text]
    39. 39
  39. Wang, H., F. E. McCann, J. D. Gordan, X. Wu, M. Raab, T. H. Malik, D. M. Davis, and C. E. Rudd. 2004. ADAP-SLP-76 binding differentially regulates supramolecular activation cluster (SMAC) formation relative to T cell-APC conjugation. J. Exp. Med. 200:1063-1074.[Abstract/Free Full Text]
    40. 40
  40. Wang, H., E. Y. Moon, A. Azouz, X. Wu, A. Smith, H. Schneider, N. Hogg, and C. E. Rudd. 2003. SKAP-55 regulates integrin adhesion and formation of T cell-APC conjugates. Nat. Immunol. 4:366-374.[CrossRef][Medline]
    41. 41
  41. Wilkinson, B., H. Wang, and C. E. Rudd. 2004. Positive and negative adaptors in T-cell signalling. Immunology 111:368-374.[CrossRef][Medline]
    42. 42
  42. Wu, J. N., S. Gheith, N. A. Bezman, Q. H. Liu, L. V. Fostel, A. M. Swanson, B. D. Freedman, G. A. Koretzky, and E. J. Peterson. 2006. Adhesion- and degranulation-promoting adapter protein is required for efficient thymocyte development and selection. J. Immunol. 176:6681-6689.[Abstract/Free Full Text]
    43. 43
  43. Wu, L., J. Fu, and S. H. Shen. 2002. SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription. Mol. Cell. Biol. 22:2673-2686.[Abstract/Free Full Text]
    44. 44
  44. Yablonski, D., and A. Weiss. 2001. Mechanisms of signaling by the hematopoietic-specific adaptor proteins, SLP-76 and LAT and their B cell counterpart, BLNK/SLP-65. Adv. Immunol. 79:93-128.[Medline]
    45. 45
  45. Zambrowicz, B. P., G. A. Friedrich, E. C. Buxton, S. L. Lilleberg, C. Person, and A. T. Sands. 1998. Disruption and sequence identification of 2,000 genes in mouse embryonic stem cells. Nature 392:608-611.[CrossRef][Medline]

Molecular and Cellular Biology, October 2007, p. 6863-6875, Vol. 27, No. 19
0270-7306/07/$08.00+0     doi:10.1128/MCB.00556-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Horn, J., Wang, X., Reichardt, P., Stradal, T. E., Warnecke, N., Simeoni, L., Gunzer, M., Yablonski, D., Schraven, B., Kliche, S. (2009). Src Homology 2-Domain Containing Leukocyte-Specific Phosphoprotein of 76 kDa Is Mandatory for TCR-Mediated Inside-Out Signaling, but Dispensable for CXCR4-Mediated LFA-1 Activation, Adhesion, and Migration of T Cells. J. Immunol. 183: 5756-5767 [Abstract] [Full Text]  
  • Wang, H., Wei, B., Bismuth, G., Rudd, C. E. (2009). SLP-76-ADAP adaptor module regulates LFA-1 mediated costimulation and T cell motility. Proc. Natl. Acad. Sci. USA 106: 12436-12441 [Abstract] [Full Text]  
  • Burbach, B. J., Srivastava, R., Medeiros, R. B., O'Gorman, W. E., Peterson, E. J., Shimizu, Y. (2008). Distinct Regulation of Integrin-Dependent T Cell Conjugate Formation and NF-{kappa}B Activation by the Adapter Protein ADAP. J. Immunol. 181: 4840-4851 [Abstract] [Full Text]  

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, H.
Right arrow Articles by Rudd, C. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, H.
Right arrow Articles by Rudd, C. E.

Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»