Author's Correction for Segal et al., Mol. Cell. Biol. 26 (13) 5120-5130.
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 Previous Article

Molecular and Cellular Biology, January 2007, p. 789-790, Vol. 27, No. 2
0270-7306/07/$08.00+0     doi:10.1128/MCB.02173-06

AUTHOR'S CORRECTION

Sbp1p Affects Translational Repression and Decapping in Saccharomyces cerevisiae

Scott P. Segal, Travis Dunckley, and Roy Parker

Department of Molecular Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, Arizona 85721

Volume 26, no. 13, p. 5120-5130, 2006. In the course of examining the experiments in this paper, we detected an error in that some of the strains used in the experiments shown in Fig. 5 and 6 were incorrect. In addition, an error was made in the preparation of Fig. 5, where a polysome tract was inadvertently used twice. To correct these errors, we reconstructed the pat1{Delta} sbp1{Delta} double mutants (yRP2168 and yRP2169) and repeated the experiments with the correct strains.


Figure 5
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  		FIG. 5.

Figure 6
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  		FIG. 6.
As presented in our now corrected Fig. 5 and 6, we observed two differences from our earlier report. First, we observed that the sbp1{Delta} strain was able to repress translation essentially like the wild-type strain. This is in contrast to our earlier report that this single mutant was partially defective in translational repression during glucose deprivation. Second, we observed that the pat1{Delta} sbp1{Delta} double mutant is still defective at translation repression but to a more modest degree than previously reported. Specifically, while the wild type undergoes ~95% repression, the pat1{Delta} sbp1{Delta} strain undergoes approximately 40 to 50% repression (in contrast to the 11% repression that we previously reported). Thus, we still saw a two- to threefold decrease in translational repression in a pat1{Delta} sbp1{Delta} strain as compared to a pat1{Delta} strain, whereas previously, we saw almost a complete loss of translational repression in the pat1{Delta} sbp1{Delta} strain.

The differences in the degree of repression do not invalidate the conclusion of the paper that Sbp1p can affect the process of translation repression and targeting of mRNAs to P-bodies for decapping, but they reduce the magnitude of the effect that sbp1{Delta} has on this process. Moreover, our reexamination of this work argues that all the other figures in this paper are still valid and accurate.

We offer our sincere apologies for any inconvenience or wasted effort these errors may have caused for members of the scientific community.

Page 5125, legend to Fig. 5, line 1: "sbp1{Delta} fails to repress translation" should read "Translational repression is affected in sbp1{Delta} strains."

Page 5126, legend to Fig. 6, line 1: "sbp1{Delta} strains" should read "pat1{Delta} sbp1{Delta} strains."

Molecular and Cellular Biology, January 2007, p. 789-790, Vol. 27, No. 2
0270-7306/07/$08.00+0     doi:10.1128/MCB.02173-06





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