Files in this Data Supplement:

• Supplemental file 1 - Fig. S1 (Localizing and isolating punctate cellular structures), S2 (Insulin increases a population of immobile GLUT4-containing structures in the TIRF evanescence field), S3 (Punctate GLUT4 structures colocalize with immobile clathrin-coated membranes in quiescent 3T3-L1 adipocytes), S4 (Insulin promotes GLUT4 accumulation in immobile clathrin-coated membranes), and S5 (Kinetics of single GLUT4 vesicle docking and fusion with the PM) and figure and movie legends
  PDF document, 523K.
• Supplemental file 2 - Movies S1 and S2 (Insulin stimulates GLUT4 exocytosis S1 and promotes apparent immobilization of punctate GLUT4 structures S2 in the TIRF evanescence field), S3 (Dynamic GLUT4 structures in the TIRF evanescence field), S4 (Clathrin-coated pits have limited lateral mobility in the TIRF evanescence field), and S5 (Dynamic GLUT4 structures in the TIRF evanescence field)
  Zipped MOV document, 19MB.
• Supplemental file 3 - Movies S6 (Clathrin-coated pits have limited lateral mobility in the TIRF evanescence field), S7 and S8 (Representative single GLUT4 vesicle fusion in an insulin-stimulated S7 and quiescent S8 3T3-L1 adipocyte), S9 (Candidate fusion events identified by Fusion Assistant), and S10 (Visually confirmed single-vesicle docking/fusion events)
  Zipped MOV document, 20MB.