Files in this Data Supplement:

• Supplemental file 1 - Legends to Fig. S1 to S5 and Tables S1 (NICDΔC species generated in the cell-free assay) and S2 (Sequence alignment of putative transmembrane domains of various Notch orthologs)
  MS Word document, 55K.
• Supplemental file 2 - Fig. S1 PS/γ-secretase generates the identified NICD(ΔC) species in the cell-free Notch-1 cleavage assay, S2 (Preparation of NICD-V and NICD-S polypeptides and their capping antibodies), S3 Coloading of β-galactosidase and NICD-V or NICD-S(+3), S4 (Regulation of the rate of endocytosis by bafilomycin A1 causes a change in the precision of S3 cleavage), and S5 (Specificity and sensitivity of anti-NT-L)
  Zipped PDF document, 155K.