Files in this Data Supplement:

• Supplemental file 1 - Supplemental figure legends and references.
  Zipped MS Word document, 8K.
• Supplemental file 2 - Table S1 (IC₅₀ values for inhibition of FANCJ helicase activity by selected small molecules).
  Zipped MS Word document,3K.
• Supplemental file 3 - Fig. S1 (SDS-PAGE and Coomassie stain of proteins used).
  Zipped TIF file, 296K.
• Supplemental file 4 - Fig. S2 (FANCJ efficiently unwinds G4 DNA in a protein concentration-dependent manner).
  Zipped TIF file, 228K.
• Supplemental file 5 - Fig. S3 ( RECQ1 fails to unwind G4 DNA).
  Zipped TIF file, 326K.
• Supplemental file 6 - Fig. S4 (G4-interactive molecule telomestatin potently inhibits BLM G4 helicase) .
  Zipped TIF file, 204K.
• Supplemental file 7 - Fig. S5 (Effects of small molecules NMM and T4 on FANCJ helicase activity).
  Zipped TIF file, 26K.
• Supplemental file 8 - Fig. S6 (FANCJ depletion cells sensitizes to MMC and TMS).
  Zipped TIF file, 29K.
• Supplemental file 9 - Fig. S7 (FA-A and FA-D2 mutant cell lines are not sensitive to TMS).
  Zipped TIF file, 20K.
• Supplemental file 10 - Fig. S8 (Telomestatin induces elevated γ-H2AX foci in FANCJ-depleted HeLa cells).
  Zipped TIF file, 417K.