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Regulation of ABCG2 Expression at the 3' Untranslated Region of Its mRNA through Modulation of Transcript Stability and Protein Translation by a Putative MicroRNA in the S1 Colon Cancer Cell Line
Mol. Cell. Biol. To et al. 28: 5147

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Fig. S1 (mRNA stability study for ABCG2).
    Zipped EPS file, 274K.
  • Supplemental file 2 - Fig. S2 (Analysis of ABCG2 mRNA stability by quantitative real-time PCR).
    Zipped EPS file, 339K.
  • Supplemental file 3 - Fig. S3 (3’RACE assay).
    Zipped EPS file, 298K.
  • Supplemental file 4 - Fig. S4 (Calibration curves for PCR analysis of the relative abundance of ABCG2 3'UTR fragments).
    Zipped EPS file, 471K.
  • Supplemental file 5 - Fig. S5 (PCR analysis of mRNA stability of ABCG2 3’UTR fragments).
    Zipped EPS file, 337K.
  • Supplemental file 6 - Fig. S6 (Analysis of the relative abundance and mRNA stability of ABCG2 3'UTR fragments by quantitative real-time PCR using Sybr green assay).
    Zipped EPS file, 336K.
  • Supplemental file 7 - Fig. S7 (Sequence analysis of the 3’UTR of ABCG2).
    Zipped EPS file, 2MB.
  • Supplemental file 8 - Fig. S8 (Human ABCG2 mRNA secondary structure predicted by mFold).
    Zipped EPS file, 258K.
  • Supplemental file 9 - Fig. S9 (Luciferase reporter constructs used to study the 3'UTR of ABCG2 mRNA).
    Zipped EPS file, 251K.
  • Supplemental file 10 - Fig. S10 (miR-519c binding sequence in ABCG2 3'UTR with luciferase reporter assay).
    Zipped EPS file, 219K.
  • Supplemental file 11 - Fig. S11 (Presence of long ABCG2 3’UTR transcript in S1 cells demonstrated by 3’RACE assay).
    Zipped EPS file, 280K.
  • Supplemental file 12 - Fig. S12 (miR-519c binding site facilitates regulation of ABCG2 expression by mRNA degradation and protein translation blockade).
    Zipped EPS file, 332K.
  • Supplemental file 13 - Legends to Fig. S1 to S12.
    MS Word document, 42K.




This Article
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