
Regulation of ABCG2 Expression at the 3' Untranslated Region of Its mRNA through Modulation of Transcript Stability and Protein Translation by a Putative MicroRNA in the S1 Colon Cancer Cell Line
Mol. Cell. Biol. To et al.
28: 5147
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
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Fig. S1 (mRNA stability study for ABCG2).
Zipped EPS file, 274K.
- Supplemental file 2
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Fig. S2 (Analysis of ABCG2 mRNA stability by quantitative real-time PCR).
Zipped EPS file, 339K.
- Supplemental file 3
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Fig. S3 (3’RACE assay).
Zipped EPS file, 298K.
- Supplemental file 4
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Fig. S4 (Calibration curves for PCR analysis of the relative abundance of ABCG2 3'UTR fragments).
Zipped EPS file, 471K.
- Supplemental file 5
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Fig. S5 (PCR analysis of mRNA stability of ABCG2 3’UTR fragments).
Zipped EPS file, 337K.
- Supplemental file 6
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Fig. S6 (Analysis of the relative abundance and mRNA stability of ABCG2 3'UTR fragments by quantitative real-time PCR using Sybr green assay).
Zipped EPS file, 336K.
- Supplemental file 7
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Fig. S7 (Sequence analysis of the 3’UTR of ABCG2).
Zipped EPS file, 2MB.
- Supplemental file 8
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Fig. S8 (Human ABCG2 mRNA secondary structure predicted by mFold).
Zipped EPS file, 258K.
- Supplemental file 9
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Fig. S9 (Luciferase reporter constructs used to study the 3'UTR of ABCG2 mRNA).
Zipped EPS file, 251K.
- Supplemental file 10
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Fig. S10 (miR-519c binding sequence in ABCG2 3'UTR with luciferase reporter assay).
Zipped EPS file, 219K.
- Supplemental file 11
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Fig. S11 (Presence of long ABCG2 3’UTR transcript in S1 cells demonstrated by 3’RACE assay).
Zipped EPS file, 280K.
- Supplemental file 12
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Fig. S12 (miR-519c binding site facilitates regulation of ABCG2 expression by mRNA degradation and protein translation blockade).
Zipped EPS file, 332K.
- Supplemental file 13
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Legends to Fig. S1 to S12.
MS Word document, 42K.