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Noncanonical E2 Variant-Independent Function of UBC13 in Promoting Checkpoint Protein Assembly
Mol. Cell. Biol. Huen et al. 28: 6104

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Fig. S1 (Levels of ubiquitylated H2AX analyzed by immunoblotting), S2 (Immunostaining of RNF8- and UBC13-deficient cells), S3 (Localization of fusion proteins to DSBs in response to IR), S4 (Ring domain mutants failed to restore RNF8 function in promoting 53BP1 and FK2 IRIFs), S5 (UBC13 E55Q and F57E mutations abolish interaction with its E2 variants MMS2 and UEV1A), S6 (UBC13 is required for IRIF formation of 53BP1 and FK2 but not that of pH2AX), S7 (MMS2-depleted cells display elevated levels of DNA damage), and S8 (Rad18 is not required for 53BP1 and FK2 IRIF formation).
    PDF file, 4.8MB.



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