
Activation of the SPS Amino Acid-Sensing Pathway in Saccharomyces cerevisiae Correlates with the Phosphorylation State of a Sensor Component, Ptr3
Mol. Cell. Biol. Liu et al.
28: 551
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Supplemental text; Fig. S1 (Mutations in five CKI consensus sites of PTR3 do not significantly affect AGP1-lacZ reporter gene expression), S2 (An rts1;delta mutation does not activate AGP1-lacZ expression in cocultured wild-type cells carrying an AGP1-lacZ reporter gene), S3 (The threonine 525-to-alanine mutation in Ptr3 does not affect its interaction with Ssy5-C), S4 (Mutations in SSY1 and PTR3 do not increase instability of Ssy5-C), and S5 (An N-terminal 6xHA-tagged Ssy5 is not constitutively active); and Tables S1 (Strains used) and S2 (Plasmids used)
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