Molecular and Cellular Biology, November 2008, p. 6730, Vol. 28, No. 21
0270-7306/08/$08.00+0 doi:10.1128/MCB.01333-08
| AUTHOR'S CORRECTION |
Mechanism of Histone H1-Stimulated Glucocorticoid Receptor DNA Binding In Vivo
Sergey Belikov, Carolina Åstrand, and Örjan WrangeDepartment of Cell and Molecular Biology, Karolinska Institutet, SE-17177 Stockholm, Sweden
Volume 27, no. 6, p. 2398-2410, 2007. The subtype of linker histone H1 primarily used in this work was Xenopus H1o (xH1o) and not Xenopus H1A (xH1A) as was erroneously stated in the report. We sincerely apologize for this error; however, it does not affect the conclusions on the mechanism of linker histone H1-stimulated glucocorticoid receptor binding made in this work since we find the same qualitative effects of xH1A as shown to occur with xH1o. Specifically, experiments demonstrated in Fig. 1 to 5, 7B, and 8 and Supplement S1 in the supplemental material were conducted with xH1o; however, the chromatin immunoprecipitation experiments in Fig. 6C and 7A and Supplements S2 and S3 in the supplemental material were indeed done with an N-terminally hemagglutinin (HA)-tagged xH1A construct. The experiment in Supplement S3 in the supplemental material demonstrates that wild-type H1o and HA-H1A compete for the same binding site in chromatin. This argues that the effects are general linker histone effects.
The estimation of the H1/nucleosome (H1/N) ratio was based on the incorporation of [14C]lysine after mRNA injection; since xH1o harbors 54 lysine residues while xH1A has 64 lysine residues, the H1/N ratios should be 19% higher than indicated (
1.19-fold higher) in Fig. 1 to 5 and 8 and Supplements S1 and S3 in the supplemental material. This is within the error of this semiquantitative estimation.
Page 2398, abstract, line 3: "H1A" should read "H1o."
Page 2399, column 1, line 18: "H1A" should read "H1o."
Page 2399, column 1, Materials and Methods, line 6: "...DQ466082), and..." should read "...DQ466082), plasmids for production of mRNA for histone H1 were from a PCR-amplified xH1 cDNA clone (kindly provided by K. Ura, accession number Z71503), and...."
Page 2399, Fig. 1B: "H1A" should read "H1" in both places.
Page 2399, legend to Fig. 1, lines 5, 8, and 11: "H1A" should read "H1."
Page 2400, column 1, line 14: "HA-H1A, dubbed HA-H1, was" should read "HA-H1A was."
Page 2405, column 1, lines 35, 36 (twice), 39, 43 (twice), 44, 51, and 57: "H1" should read "H1A."
Page 2405, column 2, lines 4 and 7: "H1" should read "H1A."
Page 2405, legend to Fig. 6, line 4 from bottom: "HA-H1 mRNA" should read "HA-H1A mRNA (HA-H1)."
Page 2406, legend to Fig. 7, line 1: "H1" should read "H1A."
Page 2406, legend to Fig. 7, line 3: "HA-H1 mRNA" should read "HA-H1A mRNA (HA-H1)."
Page 2406, column 2, lines 1 and 3: "H1" should read "H1A."
Page 2408, column 2, lines 3 and 1 from bottom: "H1" should read "H1A."
Revised supplemental material, in which the H1 labeling in Supplements S2 and S3 is corrected, is posted at http://mcb.asm.org/cgi/content/full/27/6/2398/DC1.
Molecular and Cellular Biology, November 2008, p. 6730, Vol. 28, No. 21
0270-7306/08/$08.00+0 doi:10.1128/MCB.01333-08
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