Files in this Data Supplement:

• Supplemental file 1 - Fig. S1 (Protein depletion in siRNA-treated cells), S2 (Supernumerary asters in Tog-kd cells), S3 (Ch-Tog in GFP-tubulin signal recovery following photobleaching), S4 (Multipolar spindles in Tog-kd cells and ch-Tog on spindle MTs in TACC3-kd cells), S5 (EB1 levels at spindle poles of Tog-kd and TACC3-kd cells), S6 (Acetylated tubulin in centrosomes of Tog-kd cells), S7 (Kinetochore fibers in Tog-kd cells), S8 (Distance between centrioles and centrosomal localization of pericentrin in Tog-kd cells), and S9 (TACC3 on the plus ends of microtubules); Table S1 (Target sequences of siRNA duplexes used); and legends to Movies S1 to S4.
  PDF file, 4MB.
• Supplemental file 2 - Movie S1 (Bipolar spindle assembly in GFP-tubulin-expressing Con-kd cell).
  Zipped MOV file, 1MB.
• Supplemental file 3 - Movie S2 (Multipolar spindle formation in GFP-tubulin-expressing Tog-kd cell).
  Zipped MOV file, 269K.
• Supplemental file 4 - Movie S3 (Multipolar spindle formation in GFP-tubulin-expressing Tog-kd cell).
  Zipped MOV file, 552K.
• Supplemental file 5 - Movie S4 (Bipolar spindle formation in GFP-tubulin-expressing MCAK/Tog-kd cell).
  Zipped MOV file, 379K.
• Supplemental file 6 - Movie S5 (Multipolar spindle formation in GFP-tubulin-expressing Tog-kd cell).
  Zipped MOV file, 99K.