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The JmjC Domain Histone Demethylase Ndy1 Regulates Redox Homeostasis and Protects Cells from Oxidative Stress
Mol. Cell. Biol. Polytarchou et al. 28: 7451

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Fig. S1 (Ndy1 construct expression and localization), S2 (Ndy1 demethylates dimethylated histone H3), S3 (Ndy1 overexpression and H2O2-induced oxidative stress), S4 (Ndy1, Nqo1, and Prdx4 expression in H2O2-treated MEFs), S5 (Ndy1 depletion and dG oxidation), S6 (Ndy1 effect on superoxide ion accumulation and mitochondrial ROS levels), S7 (Cellular H2O2 levels), S8 (Effect of excision of overexpressed Ndy1 on Nqo1 and Prdx4 expression), S9 (Ndy1 overexpression and Nqo1 transcriptional activation), S10 (Ndy1- ΔCXXC effect on cellular H2O2 levels and oxidative stress), and S11 (ChIP for Ndy1 and histone H3 K36me2 associated with the Prdx2 promoter) and Tables S1 and S2 (siRNA [S1] and RT-PCR primer [S2] sequences) and S3 to S5 (Nqo1 [S3], Prdx4 [S4], and Prdx2 [S5] primer sets used for ChIP).
    Zipped MS Word document, 3MB.




This Article
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