Files in this Data Supplement:

• Supplemental file 1 - Tables S1 and S2 (Primers for amplification of GTPases [S1] and semiquantitative RT-PCR [S2]), and S3 (siRNA target sequences of Cdc42/Rac family GTPases); supplemental text; and legends to Fig. S1 to S7
  Word doc, 76KB.
• Supplemental file 2 - Fig. S1 (Procedure for simultaneous analysis of scrambled and GTPase-specific siRNA-transfected cell populations), S2 (Efficacies of siRNAs in blocking expression of EGFP fusions), S3 (Scans of autoradiograph), S4 (C3-transferease microinjection and PDGF-dependent migration of MEF), S5 (Cy3-IgG microinjection effect on cell morphology), S6 (FMI values), and S7 (Chemotaxis of MEF towards PDGF-BB)
  Zipped TIFF files, 6.2MB.
• Supplemental file 3 - Movies S1 (Red and green dye-labelled cells), S2 (Cells with associated trajectories and angles), S3 (C3-microinjected cells), S4 and S5 (Control and Cdc42 siRNAs), S6 and S7 (Control and RAc1 siRNAs), S8 and S9 (Control and RhoG siRNAs), S10 and S11 (Control and Cdc42/Rac1 siRNAs), S12 and S13 (Control and Cdc42/Rac1/RhoG siRNAs), S14 (Scrambled siRNA), and S15 (Wortmannin)
  Zipped MOV files, 12.4MB.