Files in this Data Supplement:

• Supplemental file 1 - Movies S1 to S4 (siRNA-transfected cells)
  Zipped MPG files, 2.9MB.
• Supplemental file 2 - Fig. S1 PtdIns(3,4,5)P3-induced PI(3)K activation, S2 (GFP signals in siRNA-transfected cells), S3 (Cdc42 knockdown and misaligned chromosomes), S4 (Misoriented spindle assembly in siRNA-transfected cells), S5 (Dominant-negative Cdc42 and spindle misorientation), S6 (Cdc42 and Rac1 knockdown and cell area of metaphase HeLa cells), S7(Cdc42 or PAK2 knockdown and retraction fiber number), and S8 (Spindle formation in PAK2-depleted cells)
  Zipped PDF file, 1.8MB.
• Supplemental file 3 - Fig. S9 and S10 (PAK2 and spindle orientation in MCF10A [S9] and HeLa [S10] cells), S11 (Cdc42-dependent PAK2 localization), S12 [PI(3)K and PAK2-βPix interaction], S13 [PAK2 and PI(3)K in spindle orientation[, S14 (Spindle orientation in Cdc42- or β1 integrin-depleted cells), S15 (PAK1 and βPix in spindle orientation control), and S16 (PAK2 depletion and Aurora A activation)
  Zipped PDF file, 2.1MB.