Lysine-Independent Turnover of Cyclin G1 Can Be Stabilized by B'{alpha} Subunits of Protein Phosphatase 2A

Although the cyclin G1 gene is known to be regulated at thetranscriptional level by p53, less is understood about the turnoverof its protein product. We found that ectopically and endogenouslyexpressed cyclin G1 protein is highly unstable and is degradedby a proteasome-mediated pathway. The N-terminal 137 amino acidsof cyclin G1 (cyclin G^1-137) are necessary and sufficient forboth cyclin G1 ubiquitination and turnover. Interestingly, amutant cyclin G1 (8KR) in which all lysine residues in thisregion have been replaced with arginine can be both ubiquitinatedin cells and stabilized by a proteasome inhibitor to a similarextent as wild-type cyclin G^1-137. Furthermore, the presenceof a six-Myc tag at the N terminus of cyclin G^1-137 significantlyinhibits the protein's turnover, suggesting a role for the extremeN terminus of the protein in ubiquitin-mediated proteolysis.Although we and others previously showed that cyclin G1 proteincan bind to MDM2, which functions as an E3 ubiquitin ligaseto p53 and itself, cyclin G1 protein can be degraded in cellswithout MDM2 and p53. Interestingly, the B' ${alpha}$ 1 subunit of theserine/threonine protein phosphatase 2A, which binds to cyclinG1, can stabilize cyclin G1 under unstressed conditions andupon DNA damage, as well as inhibit the ability of cyclin G1to be ubiquitinated. Our results thus indicate that proteasomalturnover of cyclin G1 is regulated by noncanonical processes.

INTRODUCTION

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INTRODUCTION

Cyclin G1 was one of the earliest p53 transcriptional targetsto be identified (41), although to this day, its roles in cellsare not fully understood. In some experimental settings, cyclinG1 may promote cell cycle arrest and apoptosis (23, 27, 42,48, 50, 62), while in others it can increase cell proliferation(18, 26, 27, 51, 63) and even tumorigenesis (7, 22). Consistentwith its possible pro-oncogenic roles, our group (43) and others(28, 31, 39, 40) have found evidence that cyclin G1 can serveas a negative regulator of p53, although it has also been reportedthat Mdm2 may downregulate growth arrest by p53 via cyclin G(62).

We previously showed that cyclin G1 binds to protein phosphatase2A (PP2A) through its ability to interact with members of theB' ${alpha}$ regulatory subunits (42). This interaction results in dephosphorylationof MDM2 at a minimum of two sites, T216 in mouse MDM2 and Ser166in human MDM2 (HDM2) (43). We also showed that MDM2 phosphorylatedat T216 binds less well to p53 (60). Correspondingly, MDM2 proteinin cyclin G1 knockout mice is hyperphosphorylated at T216, andthe levels of p53 are significantly higher than those in wild-typemouse embryonic fibroblasts (43).

Because of the complexity of its possible roles, we have investigatedhow the cyclin G1 protein itself is regulated. Cyclin G1 wasso named because it has a typical cyclin box, although it lacksthe PEST sequence that is required for degradation of the canonicalcyclins. Thus, it is not clear how cyclin G1 protein turnoveris controlled. Most proteins that are degraded by the proteasomeneed to first be conjugated to ubiquitin, a 9-kDa protein thatcan form polyubiquitination chains at an internal lysine residue(s)(19, 44). There are exceptions to this, and a small group ofproteins are known to undergo lysineless ubiquitination andinstead to have ubiquitin conjugated at the N-terminal methionine(11). Such proteins include p21 (5), the p14/19 ARF protein(29, 30), Id1 (52), Id2 (14), ERK3 (12), MyoD (6), LMP1 (1),LMP2A (21), the human papillomavirus 16 (HPV-16) oncoproteinE7 (3, 47), and HPV-58 E7 (1). Of these proteins, ARF and HPV-58E7 are the most physiologically relevant examples because theyare naturally occurring lysineless proteins that are degradedby the proteasome (29, 30), whereas for the other proteins,the mutated lysineless versions were identified as substratesfor N-terminal ubiquitination.

Here we have examined the turnover of cyclin G1 and found thatits degradation proceeds by lysineless ubiquitination and aproteasome-mediated degradation pathway that most likely involvesits N terminus. Furthermore, the B' subunit of PP2A stabilizescyclin G1 by inhibiting its proteasome-mediated degradation.

MATERIALS AND METHODS

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MATERIALS AND METHODS

Expression plasmids. Constructs containing full-length cyclin G1 cDNA (pCMV cyclinG1) and Flag-tagged human B' ${alpha}$ 1, B' ${alpha}$ 2, and B ${alpha}$ 1 cDNAs (pCMV-F-B' ${alpha}$ 1,pCMV-F-B' ${alpha}$ 2, and pCMV-F-B ${alpha}$ 1, respectively) have been described(42, 54). A hemagglutinin (HA)-tagged cyclin G1 construct waspreviously described (43). Flag-tagged human cyclin G1 was kindlyprovided by Karen Vousden, and the V5-tagged cyclin G2 cDNAexpression vector was a generous gift from Mary Horn. The PCMV-HA-ubiquitinexpression construct was obtained from D. Bohmann. BamHI-NotIsites were used to clone the human cyclin G1 cDNA into a pTRE2hygvector (Clontech). HA-tagged N-terminal (residues 1 to 137)and C-terminal (residues 136 to 294) cyclin G1 portions weregenerated by PCR, using full-length cyclin G1 cDNA as the template.Multiple lysine mutations were generated by subsequent site-directedmutagenesis, using full-length cyclin G1 or the N-terminal portion(residues 1 to 137) as the template (Invitrogen). All mutantconstructs were confirmed by sequencing.

Cells and culture conditions. NIH 3T3 cells, p53- and MDM2-null mouse 2KO cells, HeLa Tet-oncells (Clontech), and H1299 cells were cultured in Dulbecco'smodified Eagle's medium with 10% fetal bovine serum at 37°Cin 5% CO₂. Clone 6 rat cells were cultured in Dulbecco's modifiedEagle's medium with 10% fetal bovine serum at 38°C in 5%CO₂. MG132 (Sigma), E64 (Calbiochem), and cycloheximide (Sigma)were dissolved in dimethyl sulfoxide. LLnL (Sigma) was dissolvedin 100% ethanol. Doxycycline (Sigma) and neocarzinostatin (NCS)(500 ng/ml; Kayaku Co., Tokyo, Japan) were dissolved in H₂O.Transfections were performed using Lipofectamine (Invitrogen).In each experiment, 2 µg of DNA and 10 µl of Lipofectaminewere introduced into 5 x 10⁵ cells. To generate human cell lineswith inducible cyclin G1, the pTRE2-hyg human Flag-tagged cyclinG1 plasmid was transfected into HeLa Tet-on cells (Clontech),and hygromycin B (400 µg/ml; Invitrogen)-resistant cloneswere isolated. Doxycycline-inducible Flag-tagged human cyclinG1 clones were selected by immunoblotting with M2 antibody (Sigma).One of the clones that expressed human Flag-cyclin G1 tightlycontrolled by doxycycline (low-level leakage) was selected forthe experiments (clone 2), and additional clones were also characterized.

Antibodies. Polyclonal rabbit anti-cyclin G1 antibody 178 was previouslydescribed (43). The following antibodies were obtained commercially:anti-HA (H11) antibody (Covance), anti-HA (3F10) antibody (Roche),M2 monoclonal antibody (Sigma), anti-green fluorescent protein(anti-GFP) antibody (Clontech), polyclonal rabbit antibody H-46(Santa Cruz), and anti-V5 antibody (Invitrogen). The anti-mousesecondary antibody True Blot was obtained from Bioscience, andAlexa Fluor 680 or 800-conjugated goat anti-rabbit and -mouseimmunoglobulin G secondary antibodies were purchased from MolecularProbes.

Immunoprecipitation and immunoblot analysis. Cells were lysed in LB buffer containing 50 mM Tris-HCl, pH8.0, 150 mM NaCl, 1 mM EDTA, 1% NP-40, aprotinin, phenylmethylsulfonylfluoride, 1 mM EDTA, and 1 mM MgCl₂. For immunoprecipitation,cells were lysed in TEGN buffer, containing 50 mM Tris-HCl,pH 8.0, 1 mM EDTA, 10% glycerol, and 0.5% NP-40. Washing buffercontained an additional 200 mM NaCl. An Odyssey scanning systemwas used for quantitation of protein levels in immunoblots.Alexa Fluor 680 or 800-conjugated goat anti-rabbit or -mouseimmunoglobulin G secondary antibodies were used, followed byOdyssey scanning and analysis by Odyssey software, version 2.0.

In vivo ubiquitinylation assays. Cells were lysed in buffer, followed by sonication three timesfor 10 s each time. Cell lysates were either subjected to sodiumdodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)or immunoprecipitated with the indicated antibodies and proteinG-Sepharose 4 Fast (Pharmacia).

RESULTS

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RESULTS

Cyclin G1 is an unusually unstable protein. Endogenous cyclin G1 mRNA is induced rapidly upon p53 activationand is readily detectable (2). Yet we found that 30 h aftertransient transfection of a mouse cyclin G1 expression construct,cyclin G1 protein was virtually undetectable (data not shown).Expression of cyclin G1 from a recombinant retrovirus was alsoreported to be very poor (62). Nevertheless, when we lookedat an earlier time point following introduction of a mouse cyclinG1 construct into NIH 3T3 cells (20 h after transfection), wewere able to detect some cyclin G1 protein (Fig. 1). We usedthis condition to measure the half-life of cyclin G1 by treatingcells with cycloheximide for various time periods (Fig. 1).This provided an approximate half-life for cyclin G1 of 20 min.In contrast, the half-life of the related protein cyclin G2,although this protein also is not very stable, is about 45 min(Fig. 1) and is closer to that of another well-studied p53 targetprotein, p21, which was reported to have a 50-min half-life(4, 29). Thus, compared with other p53-related proteins andother common cyclins (5, 29), cyclin G1 is exceptionally unstable.

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FIG. 1. Ectopically expressed murine cyclin G1 is short-lived. NIH 3T3 cells were transfected with constructs expressing mouse cyclin G1 (cycG1) tagged with an HA epitope at the C terminus, C-terminally V5-tagged cyclin G2 (cycG2), or empty pcDNA3 vector (Pc) for 20 h. Cells were then treated with 0.1 µg/ml cycloheximide (CHX) for the indicated times prior to lysis. Equivalent amounts of protein (30 µg) were separated by 10% SDS-PAGE and subjected to immunoblotting with polyclonal antibody 178 for cyclin G1 and anti-V5 monoclonal antibody for cyclin G2. Results were quantitated using an Odyssey scanning system.

Cyclin G1 protein can be stabilized by 26S proteasome inhibitors. To further investigate the low levels of ectopically expressedcyclin G1, NIH 3T3 cells were transfected with a murine cyclinG1 expression plasmid and then treated with the 26S proteasomeinhibitors LLnL and MG132 or the protease inhibitor E-64 (Fig.2A). Cyclin G1 was stabilized by LLnL and MG132 but not by E-64.Clone 6 cells harbor a temperature-sensitive p53 mutant (A135V)in which p53 is in the mutant conformation at 38°C and istranscriptionally inactive (45). Under these conditions, cyclinG1 is not detectable. When these cells were treated with MG132for 5 h, it became possible to detect a small amount of cyclinG1 protein (Fig. 2B) (43). When cells were shifted to 32°C,which results in production of transcriptionally active p53,and thereby induction of cyclin G1 transcription (41), proteinlevels of cyclin G1 were dramatically increased. Yet in thiscase, too, cyclin G1 protein levels were further increased upontreatment of cells with MG132. Thus, both ectopically and endogenouslyexpressed rodent cyclin G1 protein turnover is regulated byproteasome-mediated degradation. To examine human cyclin G1protein turnover, we generated clones of HeLa cell lines expressingdoxycycline-inducible (Tet-on) Flag-tagged human cyclin G1,and one (clone 2) was selected for analysis (see Materials andMethods). In these cells, even uninduced human cyclin G1 proteinwas made detectable by adding MG132 (Fig. 2C). When the cellswere treated with doxycycline to induce cyclin G1, there wasa further dramatic increase of cyclin G protein upon treatmentwith the proteasome inhibitor. Thus, ectopically expressed humancyclin G1 protein is also degraded by the 26S proteasome pathway.Finally, we were also able to show that levels of endogenoushuman cyclin G1 protein in H1299 cells were increased approximatelyfivefold by MG132 treatment (Fig. 2D).

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FIG. 2. Exogenously and endogenously expressed cyclin G1 protein can be stabilized by inhibitors of the 26S proteasome. (A) NIH 3T3 cells were transfected with constructs expressing murine cyclin G1 or with empty pcDNA3 vector (Pc) for 20 h, after which cells were left untreated or treated with LLnL (25 µM), E-64 (50 µM), or MG132 (50 µM) for 5 h. Equivalent amounts of cell lysates (30 µg) were separated by 10% SDS-PAGE and immunoblotted with cyclin G1 (178) or actin antibodies, as indicated. DMSO, dimethyl sulfoxide. (B) Clone 6 rat cells expressing temperature-sensitive mutant p53 (A135V) were maintained at 38°C. Cells were shifted to 32°C for 5 h and then either left untreated or treated with 5 µM (+), 10 µM (++), or 50 µM (+++) MG132 for an additional 5 h. Cells were lysed and separated by 10% SDS-PAGE followed by immunoblotting with the indicated antibodies, as in panel A. Parallel cultures maintained at 38°C were left untreated or treated with 50 µM MG132 for 5 h and then treated as described above. (C) HeLa Tet-on cells (clone 2), engineered to express tetracycline/doxycycline-inducible human cyclin G1 as described in Materials and Methods, were plated for 24 h, followed by treatment with or without doxycycline (2 ng/ml) for 20 h. Cells were then either left untreated or treated with 25 µM MG132 for an additional 5 h prior to lysis. Equivalent amounts of cell extracts (30 µg) were separated by SDS-PAGE and immunoblotted with anti-human cyclin G1 polyclonal antibody H-46 or anti-actin antibody. (D) H1299 cells were left untreated or treated with 50 µM MG132 for 4 h prior to lysis and separation of cell extracts (50 µg) by 10% SDS-PAGE followed by immunoblotting with H-46 and antiactin antibodies.

Cyclin G1 protein is ubiquitinated in cells. We further characterized cyclin G1 degradation by examiningwhether the protein can be ubiquitinated. When H1299 cells werecotransfected with Flag-tagged human cyclin G1 and HA-ubiquitin,significant amounts of HA-ubiquitin were present in anti-Flagimmunoprecipitates (Fig. 3A). Further support that cyclin G1is ubiquitinated was obtained by ectopic expression of murinecyclin G1 along with HA-ubiquitin in NIH 3T3 cells (Fig. 3B).In this case, direct immunoblotting with the anti-cyclin G1antibody revealed multiple forms of cyclin G1 when the cellswere treated with MG132. Even in the absence of ectopicallyexpressed ubiquitin, cyclin G1 was detected as multiple speciesafter MG132 treatment. This is likely due to the conjugationof endogenous ubiquitin to cyclin G1, since without MG132 treatment,no slower-migrating forms of cyclin G1 were detected.

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FIG. 3. Ubiquitination of cyclin G1. (A) H1299 cells were cotransfected with Flag-tagged human cyclin G1 and HA-tagged ubiquitin expression plasmids for 20 h, followed by treatment with 50 µM MG132 for 4 h. Cell lysates were immunoprecipitated with anti-Flag antibody (M2) and subjected to SDS-PAGE and immunoblotting with M2 or HA antibodies. (B) NIH 3T3 cells were cotransfected with untagged mouse cyclin G1 and HA-tagged ubiquitin expression plasmids or with empty pcDNA3 vector (Pc) for 20 h. Cells were left untreated or treated with 50 µM MG132 for 4 h. Cell lysates were separated by 8% SDS-PAGE and immunoblotted with cyclin G1 (178) or actin antibodies. The middle panel shows a shorter exposure of the same blot, showing cyclin G1 protein levels. G1-Ub1 and G1-HA-Ub1 indicate a single endogenous and ectopic HA-tagged ubiquitin molecule conjugated to ectopic cyclin G1, respectively. G1-Ub2 and G1-HA-Ub2 indicate two endogenous and ectopic HA-ubiquitin molecules conjugated to ectopic cyclin G1 protein, respectively.

The N terminus of cyclin G1 is required for proteasomal degradation. Since cyclin G1 protein is ubiquitinated and undergoes activeproteasome-mediated degradation, we sought to identify whichlysine residue(s) is ubiquitin conjugated. Murine, rat, andhuman cyclin G1 share 24 conserved lysine residues. To narrowdown the critical lysine(s), we constructed two deletion mutantscomprising either the N-terminal (residues 1 to 137; cyclinG1^1-137) or C-terminal (residues 136 to 294; cyclin G1^136-294)portion of cyclin G1. Upon transfection of these constructsinto NIH 3T3 cells followed by treatment with LLnL, it was clearthat the N-terminal portion of cyclin G1 could be stabilizedmarkedly by LLnL compared with its basal level (Fig. 4A). Incontrast, there was a much greater initial quantity of the C-terminalportion of cyclin G1 than either the full-length protein orthe N-terminal portion, and LLnL treatment had no effect onits levels. We concluded that the N terminus of cyclin G1 isthe region of the protein that is targeted by the proteasomefor degradation. These data are consistent with an experimentshowing that in H1299 cells treated with MG132, it is possibleto detect His-tagged ubiquitin conjugated to full-length cyclinG1 but not to the C-terminal portion (Fig. 4B). While we cannotformally rule out that the lysines within the C-terminal portionof cyclin G can be ubiquitinated in the context of the full-lengthprotein, our results strongly indicate that this is not thecase, but rather that cyclin G1 is ubiquitinated at a site(s)within its N terminus.

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FIG. 4. The N terminus of cyclin G1 is required for its ubiquitination and degradation. (A) NIH 3T3 cells were transfected with constructs expressing HA-tagged full-length (residues 1 to 294), C-terminal (residues 136 to 294), or N-terminal (residues 1 to 137) murine cyclin G1 or empty pcDNA3 vector (Pc) for 28 h and then were left untreated or treated with LLnL (25 µM) for 12 h. Equivalent amounts of cell lysates (30 µg) were separated by 12% SDS-PAGE and immunoblotted with anti-HA, antiactin, or anti-GFP antibodies (as a transfection control). (B) NIH 3T3 cells were transfected with constructs expressing HA-tagged full-length (residues 1 to 294) and C-terminal (residues 136 to 294) murine cyclin G1 with His-tagged ubiquitin for 24 h, followed by treatment with 25 µM MG132 for 5 h. Cell lysates were analyzed by immunoprecipitation with anti-HA antibody 3F10 and then by immunoblotting with anti-His antibody or anti-HA antibody.

Ubiquitin-mediated degradation of cyclin G1 can proceed efficiently in the absence of internal lysine residues. There are eight lysine residues within amino acids 1 to 137of cyclin G1 (Fig. 5A), and these are highly conserved amongthe mouse, rat, and human homologues. To determine which lysineresidue is critical for cyclin G1 ubiquitination and degradation,a series of lysine-to-arginine substitutions were generatedin both the full-length and N-terminal versions of cyclin G1.We first made mutants with three, five, or seven lysines replacedwith arginine within the N-terminal construct and unexpectedlyfound that each of these was equivalently stabilized by LLnL(Fig. 5B). We then constructed a series of K-to-R mutants inthe context of full-length cyclin G1 in which four, six, orall eight of the lysines within the N-terminal 137 amino acidswere mutated and again, surprisingly, found that LLnL treatmentstabilized each, including the 8KR substitution mutant, to asimilar extent to that with wild-type cyclin G1 (Fig. 5C). Furthermore,the truncated cyclin G1 construct [cyclin G1^{1-137 (8KR)}] inwhich all lysines were mutated showed similar stabilizationand ubiquitination to those of the wild-type version of cyclinG1^1-137 (Fig. 5D). In fact, the cyclin G1^{1-137 (8KR)} mutantwas conjugated to both endogenous ubiquitin and ectopic HA-ubiquitinto similar levels to those with wild-type cyclin G1 (Fig. 5D).Thus, although the N-terminal portion of cyclin G1 can be targetedefficiently by the proteasome for degradation, none of its eightlysine residues are apparently required for the ubiquitinationand degradation of cyclin G1.

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FIG. 5. All lysines in the N terminus of cyclin G1 are dispensable for its degradation and ubiquitination. (A) Positions of the eight lysine residues in the N-terminal 137 amino acids of the murine cyclin G1 protein. (B) NIH 3T3 cells were transfected with constructs expressing an N-terminal version (residues 1 to 137) of wild-type or lysine substitution (3KR, 5KR, and 7KR) mutant cyclin G1 or with empty pcDNA3 vector (Pc) for 28 h and then were left untreated (–) or treated (+) with LLnL (+) (25 µM) for 12 h. Cell extracts (30 µg) were separated by 12% SDS-PAGE and immunoblotted with anti-cyclin G1 (178), anti-GFP, or antiactin antibodies. For N-terminal (residues 1 to 137) cyclin G1 substitution mutants, 3KR represents K11R, K29R, and K34R mutations; 5KR represents K11R, K29R, K34R, K56R, and K85R mutations; and 7KR represents K11R, K29R, K34R, K56R, K85R, K87R, and K91R mutations. (C) NIH 3T3 cells were transfected with constructs expressing full-length versions of wild-type or N-terminal lysine substitution (4KR, 6KR, or 8KR) mutant cyclin G1 or with empty pcDNA3 vector (Pc) for 28 h and then were left untreated (–) or treated with LLnL (+) (25 uM) for 12 h. Cell extracts (30 µg) were separated by 10% SDS-PAGE and immunoblotted with anti-cyclin G1 (178) or antiactin antibodies. The graph shows the increase (fold change) in wild-type and mutant cyclin G1 proteins compared with untreated levels, as analyzed by an Odyssey scanning system. For full-length cyclin G1 substitution mutants, 4KR represents K11R, K29R, K34R, and K56R mutations; 6KR represents K11R, K29R, K34R, K56R, K85R, and K87R mutations; and 8KR represents K11R, K29R, K34R, K56R, K85R, K87R, K91R, and K106R mutations. (D) NIH 3T3 cells were transfected with wild-type or mutant (8KR) N-terminal (residues 1 to 137) cyclin G1 as in panel C, with or without HA-ubiquitin, for 24 h, followed by treatment with MG132 (25 µM) for 5 h. Cell lysates were separated by 12% SDS-PAGE and immunoblotted with anti-cyclin G1 (178) or antiactin antibodies. The middle panel shows a shorter exposure of the cyclin G1 portion of the blot. G1-Ub1, G1-Ub2, G1-HA-Ub, and G1-HA-Ub2 are described in the legend to Fig. 3B.

Adding an extra sequence at the N terminus of cyclin G1 inhibits its degradation. As mentioned earlier, p21 and ARF have been shown to undergolysineless proteasomal degradation and can be conjugated toubiquitin at their N termini (5, 29, 30). One line of evidenceto support this is that both proteins can be stabilized by theaddition of six copies of the Myc tag (Met-Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-Asn-Glu)fused to the N-terminal methionine (5). Presumably, the six-Myc-taggedprotein blocks the recognition site for ubiquitin or ubiquitinligase. To test whether the addition of an N-terminal tag similarlystabilizes cyclin G1, we generated a construct expressing aminoacids 1 to 137 of cyclin G1 with a six-Myc tag fused to theN-terminal methionine. Indeed, as shown in Fig. 6A, we foundthat six-Myc-tagged cyclin G^1-137 has a longer half-life (60min) in transfected NIH 3T3 cells than that of untagged cyclinG1^1-137 (30 min). Furthermore, six-Myc-tagged cyclin G1^1-137was stabilized to a lesser extent by MG132 treatment (about1.5-fold) (Fig. 6B) than was nontagged cyclin G1^1-137 (about10- to 25-fold) (Fig. 6B). This indicates that ubiquitin conjugationto cyclin G1 is inhibited by the extra sequence at the N terminus,even though endogenous ubiquitin and ectopic HA-ubiquitin areconjugated to the modified cyclin G1^1-137 in the presence ofMG132 (Fig. 6B). This could be related to the fact that thereis one lysine residue in each Myc tag. Nevertheless, our datasuggest that when it is unmodified, the N terminus of cyclinG1 is the ubiquitin conjugating site for cyclin G1 degradation.

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FIG. 6. A six-Myc epitope tag at the N terminus stabilizes cyclin G1^1-137. (A) NIH 3T3 cells were transfected with six-Myc-tagged cyclin G1^1-137 or untagged cyclin G1^1-137 for 24 h, followed by treatment with cycloheximide (0.1 µg/ml). Cells were lysed at the indicated times posttreatment, and lysates were separated by 12% SDS-PAGE followed by immunoblotting with cyclin G1 and actin antibodies as described above. Cyclin G1 protein was quantitated using an Odyssey system. (B) NIH 3T3 cells were transfected with constructs expressing six-Myc-tagged and untagged cyclin G1^1-137, with or without HA-ubiquitin, for 24 h and then either left untreated or treated with 25 µM MG132 in dimethyl sulfoxide for 5 h. Cell lysates were separated by 12% SDS-PAGE and immunoblotted with anti-cyclin G1, antiactin, or anti-GFP antibodies, as indicated on the right. On the left are indicated one or two molecules of untagged endogenous ubiquitin (Ub1 and Ub2) or ectopic HA-tagged ubiquitin (HA-Ub1 and HA-Ub2) conjugated to cyclin G1. Long and short exposures of the immunoblot of cyclin G1 are indicated.

Proteasome-mediated degradation of cyclin G1 is p53 and MDM2 independent. MDM2, the main negative regulator of p53, is an E3 ubiquitinligase (15, 20, 46, 55). Under normal growth conditions, p53protein levels are kept low by MDM2 targeting p53 for proteasomedegradation. MDM2 can also function as a ubiquitin E3 ligasefor itself. Previously, we showed that MDM2 and cyclin G1 canbind each other in vitro and in vivo and that the acidic regionand the RING domain of MDM2 are involved in the interaction(43). To investigate whether MDM2 plays a role in cyclin G1turnover, we first used a mouse cell line (2KO) in which bothp53 and MDM2 are homozygously deleted (35, 38, 53). In thesecells, the N-terminal cyclin G1^1-137 was stabilized by LLnL(Fig. 7A). To test whether human MDM2 can target human cyclinG1 for degradation, cyclin G1-inducible HeLa cells were transfectedwith increasing amounts of MDM2 and cells were collected at24 and 42 h posttransfection. Doxycycline-induced cyclin G1protein levels were not affected by Mdm2 at the 24-h time point(Fig. 7B) and were only marginally impacted after 42 h. As expected,Mdm2 targeted coexpressed p53 for degradation in the same cells(Fig. 7C). Based on this observation, it is possible that undersome conditions Mdm2 can target cyclin G1 for degradation, butthe ratio of cyclin G1 and Mdm2 may be very critical for thisto occur. This is not likely to be a key activity of MDM2, sincewhen we introduced small interfering RNA against Mdm2 into p53-nullH1299 cells, there was a significant increase in p21, previouslyshown to be an Mdm2 target for degradation (24, 53), but therewas no detectable increase in cyclin G1 protein (data not shown).

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FIG. 7. Cyclin G1 degradation by the 26S proteasome is p53 and MDM2 independent. (A) MDM2^–/– p53^–/– (2KO) cells were transfected with the N-terminal cyclin G1^1-137 construct or empty pcDNA3 vector (Pc) for 28 h and either left untreated or treated with LLnL (25 µM) for 12 h. Cell extracts (30 µg) were separated by 12% SDS-PAGE and analyzed by immunoblotting. (B) HeLa Tet-on cells expressing human cyclin G1 (clone 2) were left untreated or treated with doxycycline (2 ng/ml) and, at the same time, were transfected with increasing amounts of Flag-HDM2 for 24 h (0.5 µg, 1.0 µg, and 2.0 µg) or 42 h (0.2 µg, 0.5 µg, 1.0 µg, and 2.0 µg) or with empty pcDNA3 vector (Pc). Cell lysates were separated by 10% SDS-PAGE and analyzed by immunoblotting with anti-Flag (M2), anti-human cyclin G1 (H-46), or antiactin antibodies. (C) HeLa Tet-on cells (clone 2) without doxycycline were transfected with 0.3 µg of p53-expressing plasmid, with or without 1.5 µg of HDM2-expressing plasmid, for 24 h. Cell lysates were separated by 10% SDS-PAGE and analyzed by immunoblotting with anti-Flag (M2), anti-p53 (DO-1), or anti-GFP antibodies.

Cyclin G1 protein turnover is negatively regulated by the B' ${alpha}$ 1 subunit of PP2A. While free p21 and ARF can be targeted by the 26S proteasome,binding of these proteins to cdk2, cdk4 (p21), and B23 (ARF)stabilizes them (5, 29). As mentioned above, members of theB' ${alpha}$ subfamily of PP2A phosphatase can interact with cyclin G1.The interaction between cyclin G1 and B' ${alpha}$ 1 is reasonably strong,as the cyclin G-B' ${alpha}$ 1 complexes are stable even in 2% NP-40 and400 mM NaCl (42), and the majority of cyclin G1 protein is associatedwith high-molecular-weight complexes containing PP2A (42, 43).We therefore considered that members of the B' ${alpha}$ family may regulatecyclin G1 turnover. To test this hypothesis, ectopic Flag-taggedB' ${alpha}$ proteins were introduced into HeLa cells expressing induciblehuman cyclin G, and their impact on cyclin G1 protein levelswas assessed. Indeed, cyclin G1 levels were markedly increasedby Flag-B' ${alpha}$ 1 under both noninduced and doxcycline-induced conditions(Fig. 8A). Augmentation of cyclin G1 by B' ${alpha}$ 1 was comparable tothe effect of treating cells with MG132. Furthermore, we foundthat B' ${alpha}$ 2 expression increased cyclin G1 levels to the same extentas B' ${alpha}$ 1 (Fig. 8B), whereas another version of the PP2A B subunit(B ${alpha}$ ) that cannot bind cyclin G1 had no such stabilizing effect.The effect of the B' ${alpha}$ subunit was p53 and MDM2 independent, sincethe same results were obtained with 2KO cells (Fig. 8C). Notably,the 8KR mutant form of full-length cyclin G1, described in Fig.5, was stabilized to the same extent as wild-type cyclin G1.Most importantly, evidence that cyclin G1 proteasomal turnoverwas regulated by B' ${alpha}$ was derived from experiments showing thatthe half-life of cyclin G1 was greatly increased in the presenceof B' ${alpha}$ (Fig. 8D) and that association of ectopically expressedHA-ubiquitin with cyclin G1 was markedly reduced in the presenceof B' ${alpha}$ 1 but not B ${alpha}$ (Fig. 8E). Interestingly, B' ${alpha}$ 1 did not stabilizeeither the N-terminal (residues 1 to 137) or C-terminal (residues136 to 294) portion of cyclin G1 (data not shown), suggestingthat it requires interaction with both parts of the cyclin G1protein. This is consistent with the observation that unlikefull-length cyclin G1, neither cyclin G1^1-137 nor cyclin G1^136-294can form stable interactions with B' ${alpha}$ 1 (data not shown). Takentogether, these data demonstrate that the B' ${alpha}$ 1 and B' ${alpha}$ 2 regulatorysubunits of PP2A stabilize cyclin G1 by inhibiting its ubiquitinationand degradation by the 26S proteasome.

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FIG. 8. B' ${alpha}$ family subunits of PP2A stabilize human cyclin G1 protein. (A) HeLa Tet-on cells (clone 2) that were untreated or treated with doxycycline (Dox; 2 ng/ml) were transfected with empty pcDNA3 vector (Pc) or a vector expressing the Flag-tagged B' ${alpha}$ 1 subunit of PP2A (B' ${alpha}$ 1) for 42 h. Equivalent amounts of cell lysates (30 µg) were separated by 10% SDS-PAGE and immunoblotted (IB) with anti-Flag (M2), anti-human cyclin G1 (H-46), or antiactin antibodies. The final lane contained an extract of cells transfected with pcDNA3 and treated with doxycycline as described above, but MG132 (25 µM) was added for another 4 h. (B) HeLa Tet-on cells (clone 2) were left untreated or treated with 2 ng/ml doxycycline and, at the same time, were transfected with pcDNA3 (Pc), Flag-B' ${alpha}$ 1, Flag-B' ${alpha}$ 2, or Flag-B ${alpha}$ for 42 h. Equivalent amounts (30 µg) of cell lysates were separated by 10% SDS-PAGE and immunoblotted with anti-Flag (M2), anti-human cyclin G1 (H-46), or antiactin antibodies. (C) 2KO cells were transfected with empty pcDNA3 vector (Pc) or with constructs expressing wild-type (WT) or 8KR mutant full-length mouse cyclin G1, with or without the Flag-B' ${alpha}$ 1 subunit of PP2A, for 24 h. Cell lysates were separated by 10% SDS-PAGE and immunoblotted with anti-Flag (M2), anti-cyclin G1 (178), antiactin, or anti-GFP antibodies. (D) (Top) COS-1 cells were transfected with constructs expressing wild-type full-length mouse cyclin G1, with or without Flag-B' ${alpha}$ 1. Forty-eight hours after transfection, the transfected cells were labeled with [³⁵S]methionine for 3 h and further incubated with unlabeled medium for the indicated chase periods. Subsequently, lysates prepared from the labeled cells were used for immunoprecipitation with anti-cyclin G1 antibody (178). The immunoprecipitated cyclin G1 was then separated by 10% SDS-PAGE and processed for fluorography. (Bottom) The intensity of the labeled cyclin G1 after each chase time was quantified by densitometry, and the quantified results of the pulse-chase analyses are presented on a logarithmic scale. The presented values were calculated as follows: level of labeled cyclin G1 at the indicated time for the chase period/level of cyclin G at the start of the chase period. (E) HeLa Tet-on cells (clone 2) were left untreated (–) or treated (+) with doxycycline (Dox; 2 ng/ml) as indicated and, at the same time, were transfected with constructs expressing either empty pcDNA3 vector (Pc) or HA-ubiquitin (HA-Ub), with or without either Flag-B' ${alpha}$ 1 or Flag-B ${alpha}$ , for 24 h as indicated, followed by treatment with MG132 (25 nM) for 4 h. Equivalent amounts (1 mg) of cell lysates were immunoprecipitated with anti-cyclin G1 antibody H-46. The immunoprecipitates were separated by 10% SDS-PAGE and immunoblotted with anti-HA and anti-human cyclin G1 (H-46) antibodies. Flag-B' ${alpha}$ 1 and Flag-B ${alpha}$ were immunoblotted with anti-Flag (M2) antibody from 30 µg of cell lysates.

DNA damage-induced degradation of cyclin G1 is attenuated by the B' ${alpha}$ subunit of PP2A. Finally, we wanted to determine whether DNA damage affects theprotein stability of cyclin G1. For this purpose, we used ourHeLa cells expressing doxycycline-inducible Flag-tagged humancyclin G1. These cells were induced with doxycycline for 48h and mock treated or treated with the radiomimetic agent NCSfor 5 h, and cyclin G1 protein stability was analyzed by a cycloheximidechase. We found that the half-life of N-terminally Flag-taggedhuman cyclin G1 in untreated cells was approximately 50 min(Fig. 9A and B), similar to the half-life of six-Myc-taggedcyclin G1^1-137 (Fig. 6A). However, upon NCS treatment, the half-lifeof Flag-tagged human cyclin G1 was reduced to 15 min (Fig. 9A and B),showing that DNA damage destabilizes cyclin G1 protein. In addition,NCS destabilized ectopic mouse cyclin G1 in NIH 3T3 cells (datanot shown). More interestingly, we found that in the presenceof ectopic B' ${alpha}$ 1, NCS could not destabilize cyclin G1 comparedwith pcDNA3 vector (Fig. 9C). These data indicate that the B' ${alpha}$ 1regulatory subunit of PP2A also plays a role in stabilizingcyclin G1 in the DNA damage environment.

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FIG. 9. The B' ${alpha}$ 1 subunit of PP2A stabilizes human cyclin G1 protein under DNA damaging conditions. (A) HeLa Tet-on cells (clone 2) expressing Flag-tagged human cyclin G1 were plated with or without 2 ng/ml of doxycycline (Dox) for 48 h. The cells were either left untreated or treated with 330 ng/ml of NCS for 5 h and then were treated with cycloheximide (CHX) (0.1 µg/ml) and harvested at the indicated time points. Cell lysates (20 µg) were separated by 10% SDS-PAGE and immunoblotted with anti-cyclin G1 polyclonal (H-46) or antiactin antibodies. (B) Cyclin G1 protein was quantitated using NIH ImageJ software. (C) HeLa Tet-on cells expressing Flag-tagged human cyclin G1 were plated with or without 2 ng/ml of doxycycline (Dox) for 16 h, followed by transfection with vector pcDNA3 (Pc) or B' ${alpha}$ 1 for 24 h. NCS (330 ng/ml) was added 5 h prior to lysis. Equivalent amounts of cell lysates (30 µg) were separated by 10% SDS-PAGE and immunoblotted with the indicated antibodies.

DISCUSSION

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DISCUSSION

Most proteins whose turnover is mediated by the 26S proteasomeare ubiquitinated before being degraded by the proteasome (19).In agreement with this, we found that cyclin G1 is ubiquitinatedin cells, as determined by immunoprecipitation experiments inwhich we showed that HA-ubiquitin could be conjugated to Flag-cyclinG1 and by MG132 treatment of cells, which led to the accumulationof multiple forms of cyclin G1. A closer examination of thecyclin G1 protein revealed that the N-terminal portion, butnot the C-terminal portion, of cyclin G1 could be stabilizedby the proteasome inhibitor LLnL, and correspondingly, endogenousubiquitin could only be conjugated to the full-length and theN-terminal portion of cyclin G1.

Our further investigations into the mechanisms involved in cyclinG1 degradation revealed that cyclin G1 is not degraded throughthe internal lysine-dependent ubiquitination pathway. Strikingly,mutation of all eight internal lysine residues in the contextof the full-length or N-terminal portion of cyclin G1 did notaffect the extent to which these proteins were stabilized byLLnL or ubiquitinated, indicating that the ubiquitination anddegradation of cyclin G1 occur in a lysine-independent manner.As mentioned earlier, lysineless ubiquitination has been reportedfor other proteins. Instead, these proteins are ubiquitinatedat their N termini and the addition of an N-terminal tag stabilizesthem, most probably by blocking access of ubiquitin to the N-terminalresidue (11). Here we show that placing an extra sequence atthe N terminus of cyclin G1 stabilizes the protein and reducesits degradation by the proteasome, suggesting that cyclin G1is also ubiquitinated at its N terminus. The physiological relevanceof N-terminal ubiquitination remains to be determined. However,Sun et al. recently showed that glucocorticoids differentiallyregulate MyoD and Id1 degradation by N-terminal ubiquitinationin myotubes, demonstrating a key role for the N terminus-dependentubiquitination pathway in the physiology of muscle protein degradation(52). In addition to MyoD, which can be degraded by both thelysine-dependent and N-terminal ubiquitination pathways, multiplemechanisms of degradation have been reported for p21 (9, 10,32, 49). Deciphering the functional significance of these alternatedegradation pathways for cyclin G1 and other proteins remainsa future challenge.

The MDM2 protein, a p53 target and E3 ubiquitin ligase, is themain negative regulator of p53 (15, 16, 37). In addition top53, MDM2 functions as a ubiquitin ligase for pRB (56, 57),Numb (59), histone 2B (36), MDMX (13), and MDM2 itself (15,20). It was reported that p21 physically binds to MDM2, andalthough p21 degradation does not require MDM2 ubiquitin ligaseactivity, MDM2 promotes p21 degradation by facilitating bindingof p21 with the proteasome C8 subunit (61). ARF also binds toMDM2, although its turnover does not require MDM2 (29). We foundthat cyclin G1 binds to MDM2 at both its C-terminal RING andcentral acidic regions (43), although whether MDM2 could targetcyclin G1 for degradation was not examined. We showed here thatintermediate levels of overexpressed MDM2 had a slight impacton cyclin G1 levels. Similarly, Zhao et al. showed that cyclinG1 expression was progressively reduced as Mdm2 expression wasincreased (62). Taken together, these data suggest that perhapsMDM2 can directly (or indirectly) impact cyclin G1 degradationunder certain conditions. However, data obtained using the 2KOcell line, which lacks both the MDM2 and p53 genes, and a smallinterfering RNA against MDM2 indicate that MDM2 is unlikelyto be the primary E3 ligase for cyclin G1. It remains to bedetermined whether MDMX has a role to play in the ubiquitinationand degradation of cyclin G1.

Our findings strongly implicate PP2A as a major regulator ofcyclin G1 stability. First, increasing the levels of the regulatoryB' ${alpha}$ subunits of PP2A promotes the accumulation of cyclin G1 byinterfering with its turnover. Moreover, association of cyclinG1 with the B' ${alpha}$ subunit of PP2A inhibited its ubiquitination.In addition, B' ${alpha}$ 1 was found to stabilize cyclin G1 under DNAdamaging conditions. How might PP2A regulate the turnover ofcyclin G1? PP2A regulates the stability of other proteins, includingc-myc, the Pim family of protein kinases, and securin (17, 34,58). Each of these proteins interacts with the enzymaticallyactive PP2A and is a substrate of PP2A phosphatase. The dephosphorylationof c-myc and the Pim kinases by PP2A leads to their proteolysis,whereas PP2A stabilizes securin by removing phosphate groupsthat target the protein for degradation via the SCF ubiquitinligase. We previously showed that upon p53 induction, cyclinG1 forms a complex with enzymatically active PP2A, which dephosphorylatesMDM2 and thereby negatively regulates p53 function (43). Itremains to be determined whether cyclin G1 itself is a substrateof PP2A and if the stability of cyclin G1 is regulated by phosphorylation.

Given the complexity of proposed functions for cyclin G1, thephysiological significance of cyclin G1 stability control isnot immediately obvious. Since the discovery of cyclin G1 asa target of p53, it has been described as both a positive andnegative regulator of cell growth (8). Most recently, Joneset al. showed that cyclin G1 drives apoptosis of neural crestcells, which can be prevented by p53 inhibition (25). CyclinG1 has also been associated with genomic instability and isfrequently overexpressed in human tumors, such as cervical carcinoma(33) and human hepatocellular carcinoma (18). In addition tocyclin G1, other negative regulators of p53, including Mdm2and COP1, are also found overexpressed in tumors, and theirincreased levels have been shown to downregulate p53 function.Similar to cyclin G1, these proteins are also rapidly turnedover, and this may be important to allow full activation ofp53-dependent pathways, thereby preventing tumorigenesis. CyclinG1 is also involved in various p53-independent activities, whichmay not be surprising given the complexity of its roles in cellsand its many interacting partners. Unraveling the mechanismsinvolved in cyclin G1 stability control will likely providefurther insight into the role of cyclin G1 during normal differentiationand malignant transformation.

ACKNOWLEDGMENTS

Ella Freulich is thanked for expert technical assistance. Thanksare extended to Marshall Urist for helpful discussions and toMax Li for assistance with some experiments.

This work was supported by a C. J. Martin fellowship from theNational Health and Medical Research Council of Australia toM.J.P. and by NIH grants CA58316 and CA87497.

FOOTNOTES

* Corresponding author. Mailing address: Department of Biological Sciences, Columbia University, New York, NY 10027. Phone: (212) 854-2557. Fax: (212) 865-8142. E-mail: clp3{at}columbia.edu

Published ahead of print on 3 November 2008.

Present address: Center for Prostate Disease Research, UniformedServices University of the Health Sciences, Rockville, MD 20852.

Present address: National Cancer Center Research Institute,5-1-1 Tsukiji Bunkyo-ku, Tokyo 1040045, Japan.

REFERENCES

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