Stability of Eukaryotic Translation Initiation Factor 4E mRNA Is Regulated by HuR, and This Activity Is Dysregulated in Cancer
Mol. Cell. Biol. Topisirovic et al.
29: 1152
Supplemental material
Files in this Data Supplement:
- Supplemental file 1 -
Supplemental materials and methods and Tables S1 (Primers used for construct design) and S2 (Primers used to generate T7 RNA probes).
PDF file, 20K.
- Supplemental file 2
-
Fig. S1 (HuR binding to eIF4E mRNA and effect on eIF4E and protein A levels), S2 (HuR and nuclear export or translation of eIF4E transcripts), S3 (HuR increases the half-life of eIF4E mRNA), S4 (HuR binding to the second ARE and regulation of eIF4E transcript stability), S5 (AUR1 p42 binding to the 3′ UTR of eIF4E), and S6 (Overexpression of LacZ chimeras carrying fragments of the 3′ UTR of eIF4E and individual isoforms of AUF1).
Zipped PDF file, 7MB.
