This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Webb, C F Articles by Tucker, P W Search for Related Content PubMed PubMed Citation Articles by Webb, C F Articles by Tucker, P W

 Previous Article  |  Next Article 

Mol Cell Biol. 1991 October; 11(10): 5206-5211

Identification of a matrix-associated region 5' of an immunoglobulin heavy chain variable region gene.

Department of Immunobiology, Oklahoma Medical Research Foundation, Oklahoma City 73104.

ABSTRACT

In the accompanying report (C. F. Webb, C. Das, S. Eaton, K. Calame, and P. Tucker, Mol. Cell. Biol. 11:5197-5205, 1991), we characterize B-cell-specific protein-DNA interactions at -500 and -200 bp upstream of the mu immunoglobulin heavy chain promoter whose abundances were increased by interleukin-5 plus antigen. Because of the high A + T/G + C ratio of these sequences and the consistent findings by others that enhancer- and promoterlike regions are often located near matrix-associated regions, we asked whether these sequences might also be involved in binding to the nuclear matrix. Indeed, DNA fragments containing the -500 binding site were bound by nuclear matrix proteins. Furthermore, UV cross-linking studies showed that the DNA binding site for interleukin-5-plus-antigen-inducible proteins could also bind to proteins solubilized from the nuclear matrix. Nuclear matrix-associated sequences have also been demonstrated on either side of the intronic immunoglobulin heavy chain enhancer. Our data suggest a topological model by which interactions among proteins bound to the promoter and distal enhancer sequences might occur.

This article has been cited by other articles:

• Kim, D., Probst, L., Das, C., Tucker, P. W. (2007). REKLES Is an ARID3-restricted Multifunctional Domain. J. Biol. Chem. 282: 15768-15777 [Abstract] [Full Text]  
• Calame, K., Atchison, M. (2007). YY1 helps to bring loose ends together. Genes Dev. 21: 1145-1152 [Full Text]  
• Shankar, M., Nixon, J. C., Maier, S., Workman, J., Farris, A. D., Webb, C. F. (2007). Anti-Nuclear Antibody Production and Autoimmunity in Transgenic Mice That Overexpress the Transcription Factor Bright. J. Immunol. 178: 2996-3006 [Abstract] [Full Text]  
• Moreira, P. N., Perez-Crespo, M., Ramirez, M. A., Pozueta, J., Montoliu, L., Gutierrez-Adan, A. (2007). Effect of Transgene Concentration, Flanking Matrix Attachment Regions, and RecA-Coating on the Efficiency of Mouse Transgenesis Mediated by Intracytoplasmic Sperm Injection. Biol. Reprod. 76: 336-343 [Abstract] [Full Text]  
• Johnston, C. M., Wood, A. L., Bolland, D. J., Corcoran, A. E. (2006). Complete Sequence Assembly and Characterization of the C57BL/6 Mouse Ig Heavy Chain V Region. J. Immunol. 176: 4221-4234 [Abstract] [Full Text]  
• Kim, D., Tucker, P. W. (2006). A Regulated Nucleocytoplasmic Shuttle Contributes to Bright's Function as a Transcriptional Activator of Immunoglobulin Genes.. Mol. Cell. Biol. 26: 2187-2201 [Abstract] [Full Text]  
• Seo, J., Lozano, M. M., Dudley, J. P. (2005). Nuclear Matrix Binding Regulates SATB1-mediated Transcriptional Repression. J. Biol. Chem. 280: 24600-24609 [Abstract] [Full Text]  
• Rajaiya, J., Hatfield, M., Nixon, J. C., Rawlings, D. J., Webb, C. F. (2005). Bruton's Tyrosine Kinase Regulates Immunoglobulin Promoter Activation in Association with the Transcription Factor Bright. Mol. Cell. Biol. 25: 2073-2084 [Abstract] [Full Text]  
• Nixon, J. C., Rajaiya, J., Webb, C. F. (2004). Mutations in the DNA-binding Domain of the Transcription Factor Bright Act as Dominant Negative Proteins and Interfere with Immunoglobulin Transactivation. J. Biol. Chem. 279: 52465-52472 [Abstract] [Full Text]  
• Kaul-Ghanekar, R., Jalota, A., Pavithra, L., Tucker, P., Chattopadhyay, S. (2004). SMAR1 and Cux/CDP modulate chromatin and act as negative regulators of the TCR{beta} enhancer (E{beta}). Nucleic Acids Res 32: 4862-4875 [Abstract] [Full Text]  
• Rampalli, S., Kulkarni, A., Kumar, P., Mogare, D., Galande, S., Mitra, D., Chattopadhyay, S. (2003). Stimulation of Tat-independent transcriptional processivity from the HIV-1 LTR promoter by matrix attachment regions. Nucleic Acids Res 31: 3248-3256 [Abstract] [Full Text]  
• Goebel, P., Montalbano, A., Ayers, N., Kompfner, E., Dickinson, L., Webb, C. F., Feeney, A. J. (2002). High Frequency of Matrix Attachment Regions and Cut-Like Protein x/CCAAT-Displacement Protein and B Cell Regulator of IgH Transcription Binding Sites Flanking Ig V Region Genes. J. Immunol. 169: 2477-2487 [Abstract] [Full Text]  
• Kieffer, L. J., Greally, J. M., Landres, I., Nag, S., Nakajima, Y., Kohwi-Shigematsu, T., Kavathas, P. B. (2002). Identification of a Candidate Regulatory Region in the Human CD8 Gene Complex by Colocalization of DNase I Hypersensitive Sites and Matrix Attachment Regions Which Bind SATB1 and GATA-3. J. Immunol. 168: 3915-3922 [Abstract] [Full Text]  
• Malone, C. S., Patrone, L., Buchanan, K. L., Webb, C. F., Wall, R. (2000). An Upstream Oct-1- and Oct-2-Binding Silencer Governs B29 (Ig{beta}) Gene Expression. J. Immunol. 164: 2550-2556 [Abstract] [Full Text]  
• Sakai, E., Bottaro, A., Alt, F. W. (1999). The Ig heavy chain intronic enhancer core region is necessary and sufficient to promote efficient class switch recombination. Int Immunol 11: 1709-1713 [Abstract] [Full Text]  
• Wiersma, E. J., Ronai, D., Berru, M., Tsui, F. W. L., Shulman, M. J. (1999). Role of the Intronic Elements in the Endogenous Immunoglobulin Heavy Chain Locus. EITHER THE MATRIX ATTACHMENT REGIONS OR THE CORE ENHANCER IS SUFFICIENT TO MAINTAIN EXPRESSION. J. Biol. Chem. 274: 4858-4862 [Abstract] [Full Text]  
• Sakai, E., Bottaro, A., Davidson, L., Sleckman, B. P., Alt, F. W. (1999). Recombination and transcription of the endogenous Ig heavy chain locus is effected by the Ig heavy chain intronic enhancer core region in the absence of the matrix attachment regions. Proc. Natl. Acad. Sci. USA 96: 1526-1531 [Abstract] [Full Text]  
• WEBB, C., ZONG, R.-T., LIN, D., WANG, Z., KAPLAN, M., PAULIN, Y., SMITH, E., PROBST, L., BRYANT, J., GOLDSTEIN, A., SCHEUERMANN, R., TUCKER, P. (1999). Differential Regulation of Immunoglobulin Gene Transcription via Nuclear Matrix-associated Regions. Cold Spring Harb Symp Quant Biol 64: 109-118 [Abstract]  
• Chattopadhyay, S., Whitehurst, C. E., Chen, J. (1998). A Nuclear Matrix Attachment Region Upstream of the T Cell Receptor beta  Gene Enhancer Binds Cux/CDP and SATB1 and Modulates Enhancer-dependent Reporter Gene Expression but Not Endogenous Gene Expression. J. Biol. Chem. 273: 29838-29846 [Abstract] [Full Text]  
• Webb, C. F., Smith, E. A., Medina, K. L., Buchanan, K. L., Smithson, G., Dou, S. (1998). Expression of Bright at Two Distinct Stages of B Lymphocyte Development. J. Immunol. 160: 4747-4754 [Abstract] [Full Text]  
• Banan, M., Rojas, I. C., Lee, W.-H., King, H. L., Harriss, J. V., Kobayashi, R., Webb, C. F., Gottlieb, P. D. (1997). Interaction of the Nuclear Matrix-associated Region (MAR)-Binding Proteins, SATB1 and CDP/Cux, with a MAR Element (L2a) in an Upstream Regulatory Region of the Mouse CD8a Gene. J. Biol. Chem. 272: 18440-18452 [Abstract] [Full Text]  
• Herrscher, R F, Kaplan, M H, Lelsz, D L, Das, C, Scheuermann, R, Tucker, P W (1995). The immunoglobulin heavy-chain matrix-associating regions are bound by Bright: a B cell-specific trans-activator that describes a new DNA-binding protein family.. Genes Dev. 9: 3067-3082 [Abstract]  
• Zong, R.-T., Scheuermann, R. H. (1995). Mutually Exclusive Interaction of a Novel Matrix Attachment Region Binding Protein and the NF-µNR Enhancer Repressor. J. Biol. Chem. 270: 24010-24018 [Abstract] [Full Text]  
• Kaplan, M. H., Zong, R.-T., Herrscher, R. F., Scheuermann, R. H., Tucker, P. W. (2001). Transcriptional Activation by a Matrix Associating Region-binding Protein. CONTEXTUAL REQUIREMENTS FOR THE FUNCTION OF BRIGHT. J. Biol. Chem. 276: 21325-21330 [Abstract] [Full Text]