This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by DeMarini, D J
Right arrow Articles by Culbertson, M R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by DeMarini, D J
Right arrow Articles by Culbertson, M R

 Previous Article  |  Next Article 

Mol Cell Biol. 1992 May; 12(5): 2154-2164

SEN1, a positive effector of tRNA-splicing endonuclease in Saccharomyces cerevisiae.

D J DeMarini, M Winey, D Ursic, F Webb and M R Culbertson

Laboratory of Genetics, University of Wisconsin, Madison 53706.

ABSTRACT

The SEN1 gene, which is essential for growth in the yeast Saccharomyces cerevisiae, is required for endonucleolytic cleavage of introns from all 10 families of precursor tRNAs. A mutation in SEN1 conferring temperature-sensitive lethality also causes in vivo accumulation of pre-tRNAs and a deficiency of in vitro endonuclease activity. Biochemical evidence suggests that the gene product may be one of several components of a nuclear-localized splicing complex. We have cloned the SEN1 gene and characterized the SEN1 mRNA, the SEN1 gene product, the temperature-sensitive sen1-1 mutation, and three SEN1 null alleles. The SEN1 gene corresponds to a 6,336-bp open reading frame coding for a 2,112-amino-acid protein (molecular mass, 239 kDa). Using antisera directed against the C-terminal end of SEN1, we detect a protein corresponding to the predicted molecular weight of SEN1. The SEN1 protein contains a leucine zipper motif, consensus elements for nucleoside triphosphate binding, and a potential nuclear localization signal sequence. The carboxy-terminal 1,214 amino acids of the SEN1 protein are essential for growth, whereas the amino-terminal 898 amino acids are dispensable. A sequence of approximately 500 amino acids located in the essential region of SEN1 has significant similarity to the yeast UPF1 gene product, which is involved in mRNA turnover, and the mouse Mov-10 gene product, whose function is unknown. The mutation that creates the temperature-sensitive sen1-1 allele is located within this 500-amino-acid region, and it causes a substitution for an amino acid that is conserved in all three proteins.

Mol Cell Biol. 1992 May; 12(5): 2154-2164




This article has been cited by other articles:

  • Shimizu, K. K., Ito, T., Ishiguro, S., Okada, K. (2008). MAA3 (MAGATAMA3) Helicase Gene is Required for Female Gametophyte Development and Pollen Tube Guidance in Arabidopsis thaliana. Plant Cell Physiol 49: 1478-1483 [Abstract] [Full Text]  
  • Culbertson, M. R. (2007). Navigating Without a Road Map. Genetics 177: 1-7 [Full Text]  
  • Asaka, T., Yokoji, H., Ito, J., Yamaguchi, K., Matsushima, A. (2006). Autosomal recessive ataxia with peripheral neuropathy and elevated AFP: Novel mutations in SETX. Neurology 66: 1580-1581 [Abstract] [Full Text]  
  • Ursic, D., Chinchilla, K., Finkel, J. S., Culbertson, M. R. (2004). Multiple protein/protein and protein/RNA interactions suggest roles for yeast DNA/RNA helicase Sen1p in transcription, transcription-coupled DNA repair and RNA processing. Nucleic Acids Res 32: 2441-2452 [Abstract] [Full Text]  
  • Steinmetz, E. J., Brow, D. A. (2003). Ssu72 Protein Mediates Both Poly(A)-Coupled and Poly(A)-Independent Termination of RNA Polymerase II Transcription. Mol. Cell. Biol. 23: 6339-6349 [Abstract] [Full Text]  
  • Kufel, J., Allmang, C., Verdone, L., Beggs, J. D., Tollervey, D. (2002). Lsm Proteins Are Required for Normal Processing of Pre-tRNAs and Their Efficient Association with La-Homologous Protein Lhp1p. Mol. Cell. Biol. 22: 5248-5256 [Abstract] [Full Text]  
  • Witte, C.-P., Le, Q. H., Bureau, T., Kumar, A. (2001). Terminal-repeat retrotransposons in miniature (TRIM) are involved in restructuring plant genomes. Proc. Natl. Acad. Sci. USA 98: 13778-13783 [Abstract] [Full Text]  
  • Rasmussen, T. P., Culbertson, M. R. (1998). The Putative Nucleic Acid Helicase Sen1p Is Required for Formation and Stability of Termini and for Maximal Rates of Synthesis and Levels of Accumulation of Small Nucleolar RNAs in Saccharomyces cerevisiae. Mol. Cell. Biol. 18: 6885-6896 [Abstract] [Full Text]  
  • Wang, R.-f., O'Hara, E. B., Aldea, M., Bargmann, C. I., Gromley, H., Kushner, S. R. (1998). Escherichia coli mrsC Is an Allele of hflB, Encoding a Membrane-Associated ATPase and Protease That Is Required for mRNA Decay. J. Bacteriol. 180: 1929-1938 [Abstract] [Full Text]  
  • Shirley, R., Lelivelt, M., Schenkman, L., Dahlseid, J., Culbertson, M. (1998). A factor required for nonsense-mediated mRNA decay in yeast is exported from the nucleus to the cytoplasm by a nuclear export signal sequence. J. Cell Sci. 111: 3129-3143 [Abstract]  
  • Molnar, G. M., Crozat, A., Kraeft, S.-K., Dou, Q. P., Chen, L. B., Pardee, A. B. (1997). Association of the mammalian helicase MAH with the pre-mRNA splicing complex. Proc. Natl. Acad. Sci. USA 94: 7831-7836 [Abstract] [Full Text]  
  • He, F, Jacobson, A (1995). Identification of a novel component of the nonsense-mediated mRNA decay pathway by use of an interacting protein screen.. Genes Dev. 9: 437-454 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»