This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, Z
Right arrow Articles by Friedberg, E C
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, Z
Right arrow Articles by Friedberg, E C

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 February; 13(2): 1051-1058

DNA repair synthesis during base excision repair in vitro is catalyzed by DNA polymerase epsilon and is influenced by DNA polymerases alpha and delta in Saccharomyces cerevisiae.

Z Wang, X Wu and E C Friedberg

Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235-9072.

ABSTRACT

Base excision repair is an important mechanism for correcting DNA damage produced by many physical and chemical agents. We have examined the effects of the REV3 gene and the DNA polymerase genes POL1, POL2, and POL3 of Saccharomyces cerevisiae on DNA repair synthesis is nuclear extracts. Deletional inactivation of REV3 did not affect repair synthesis in the base excision repair pathway. Repair synthesis in nuclear extracts of pol1, pol2, and pol3 temperature-sensitive mutants was normal at permissive temperatures. However, repair synthesis in pol2 nuclear extracts was defective at the restrictive temperature of 37 degrees C and could be complemented by the addition of purified yeast DNA polymerase epsilon. Repair synthesis in pol1 nuclear extracts was proficient at the restrictive temperature unless DNA polymerase alpha was inactivated prior to the initiation of DNA repair. Thermal inactivation of DNA polymerase delta in pol3 nuclear extracts enhanced DNA repair synthesis approximately 2-fold, an effect which could be specifically reversed by the addition of purified yeast DNA polymerase delta to the extract. These results demonstrate that DNA repair synthesis in the yeast base excision repair pathway is catalyzed by DNA polymerase epsilon but is apparently modulated by the presence of DNA polymerases alpha and delta.

Mol Cell Biol. 1993 February; 13(2): 1051-1058




This article has been cited by other articles:

  • Spiga, M.-G., D'Urso, G. (2004). Identification and cloning of two putative subunits of DNA polymerase epsilon in fission yeast. Nucleic Acids Res 32: 4945-4953 [Abstract] [Full Text]  
  • Feng, W., Rodriguez-Menocal, L., Tolun, G., D'Urso, G. (2003). Schizosacchromyces pombe Dpb2 Binds to Origin DNA Early in S Phase and Is Required for Chromosomal DNA Replication. Mol. Biol. Cell 14: 3427-3436 [Abstract] [Full Text]  
  • Kelley, M. R., Kow, Y. W., Wilson, D. M. III (2003). Disparity between DNA Base Excision Repair in Yeast and Mammals: Translational Implications. Cancer Res. 63: 549-554 [Abstract] [Full Text]  
  • Pope, M. A., Porello, S. L., David, S. S. (2002). Escherichia coli Apurinic-Apyrimidinic Endonucleases Enhance the Turnover of the Adenine Glycosylase MutY with G:A Substrates. J. Biol. Chem. 277: 22605-22615 [Abstract] [Full Text]  
  • Huang, Y. (2002). Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombe. Nucleic Acids Res 30: 1465-1482 [Abstract] [Full Text]  
  • Wang, H., Elledge, S. J. (2002). Genetic and Physical Interactions Between DPB11 and DDC1 in the Yeast DNA Damage Response Pathway. Genetics 160: 1295-1304 [Abstract] [Full Text]  
  • Fuss, J., Linn, S. (2002). Human DNA Polymerase epsilon Colocalizes with Proliferating Cell Nuclear Antigen and DNA Replication Late, but Not Early, in S Phase. J. Biol. Chem. 277: 8658-8666 [Abstract] [Full Text]  
  • Li, A., Schuermann, D., Gallego, F., Kovalchuk, I., Tinland, B. (2002). Repair of Damaged DNA by Arabidopsis Cell Extract. Plant Cell 14: 263-273 [Abstract] [Full Text]  
  • Feng, W., D'Urso, G. (2001). Schizosaccharomyces pombe Cells Lacking the Amino-Terminal Catalytic Domains of DNA Polymerase Epsilon Are Viable but Require the DNA Damage Checkpoint Control. Mol. Cell. Biol. 21: 4495-4504 [Abstract] [Full Text]  
  • Wu, X., Guo, D., Yuan, F., Wang, Z. (2001). Accessibility of DNA polymerases to repair synthesis during nucleotide excision repair in yeast cell-free extracts. Nucleic Acids Res 29: 3123-3130 [Abstract] [Full Text]  
  • Balajee, A. S., Geard, C. R. (2001). Chromatin-bound PCNA complex formation triggered by DNA damage occurs independent of the ATM gene product in human cells. Nucleic Acids Res 29: 1341-1351 [Abstract] [Full Text]  
  • Vlatkovic, N., Guerrera, S., Li, Y., Linn, S., Haines, D. S., Boyd, M. T. (2000). MDM2 interacts with the C-terminus of the catalytic subunit of DNA polymerase {varepsilon}. Nucleic Acids Res 28: 3581-3586 [Abstract] [Full Text]  
  • Gomes, X. V., Gary, S. L., Burgers, P. M. J. (2000). Overproduction in Escherichia coli and Characterization of Yeast Replication Factor C Lacking the Ligase Homology Domain. J. Biol. Chem. 275: 14541-14549 [Abstract] [Full Text]  
  • Matsumoto, Y., Kim, K., Hurwitz, J., Gary, R., Levin, D. S., Tomkinson, A. E., Park, M. S. (1999). Reconstitution of Proliferating Cell Nuclear Antigen-dependent Repair of Apurinic/Apyrimidinic Sites with Purified Human Proteins. J. Biol. Chem. 274: 33703-33708 [Abstract] [Full Text]  
  • Smith, J. S., Caputo, E., Boeke, J. D. (1999). A Genetic Screen for Ribosomal DNA Silencing Defects Identifies Multiple DNA Replication and Chromatin-Modulating Factors. Mol. Cell. Biol. 19: 3184-3197 [Abstract] [Full Text]  
  • Podust, V. N., Tiwari, N., Stephan, S., Fanning, E. (1998). Replication Factor C Disengages from Proliferating Cell Nuclear Antigen (PCNA) upon Sliding Clamp Formation, and PCNA Itself Tethers DNA Polymerase delta  to DNA. J. Biol. Chem. 273: 31992-31999 [Abstract] [Full Text]  
  • Srivastava, D. K., Vande Berg, B. J., Prasad, R., Molina, J. T., Beard, W. A., Tomkinson, A. E., Wilson, S. H. (1998). Mammalian Abasic Site Base Excision Repair. IDENTIFICATION OF THE REACTION SEQUENCE AND RATE-DETERMINING STEPS. J. Biol. Chem. 273: 21203-21209 [Abstract] [Full Text]  
  • Maki, S., Hashimoto, K., Ohara, T., Sugino, A. (1998). DNA Polymerase II (epsilon ) of Saccharomyces cerevisiae Dissociates from the DNA Template by Sensing Single-stranded DNA. J. Biol. Chem. 273: 21332-21341 [Abstract] [Full Text]  
  • Mossi, R., Ferrari, E., Hubscher, U. (1998). DNA Ligase I Selectively Affects DNA Synthesis by DNA Polymerases delta  and epsilon  Suggesting Differential Functions in DNA Replication and Repair. J. Biol. Chem. 273: 14322-14330 [Abstract] [Full Text]  
  • Ellison, V., Stillman, B. (1998). Reconstitution of Recombinant Human Replication Factor C (RFC) and Identification of an RFC Subcomplex Possessing DNA-dependent ATPase Activity. J. Biol. Chem. 273: 5979-5987 [Abstract] [Full Text]  
  • Li, Y., Asahara, H., Patel, V. S., Zhou, S., Linn, S. (1997). Purification, cDNA Cloning, and Gene Mapping of the Small Subunit of Human DNA Polymerase epsilon. J. Biol. Chem. 272: 32337-32344 [Abstract] [Full Text]  
  • Wang, Z., Wu, X., Friedberg, E. C. (1997). Molecular Mechanism of Base Excision Repair of Uracil-containing DNA in Yeast Cell-free Extracts. J. Biol. Chem. 272: 24064-24071 [Abstract] [Full Text]  
  • Efrati, E., Tocco, G., Eritja, R., Wilson, S. H., Goodman, M. F. (1997). Abasic Translesion Synthesis by DNA Polymerase beta Violates the "A-rule". NOVEL TYPES OF NUCLEOTIDE INCORPORATION BY HUMAN DNA POLYMERASE beta AT AN ABASIC LESION IN DIFFERENT SEQUENCE CONTEXTS. J. Biol. Chem. 272: 2559-2569 [Abstract] [Full Text]  
  • Oda, N., Saxena, J. K., Jenkins, T. M., Prasad, R., Wilson, S. H., Ackerman, E. J. (1996). DNA Polymerases alpha and beta Are Required for DNA Repair in an Efficient Nuclear Extract from Xenopus Oocytes. J. Biol. Chem. 271: 13816-13820 [Abstract] [Full Text]  
  • Singhal, R. K., Prasad, R., Wilson, S. H. (1995). DNA Polymerase beta Conducts the Gap-filling Step in Uracil-initiated Base Excision Repair in a Bovine Testis Nuclear Extract. J. Biol. Chem. 270: 949-957 [Abstract] [Full Text]  
  • Harrington, J J, Lieber, M R (1994). Functional domains within FEN-1 and RAD2 define a family of structure-specific endonucleases: implications for nucleotide excision repair.. Genes Dev. 8: 1344-1355 [Abstract]  
  • Makiniemi, M., Hillukkala, T., Tuusa, J., Reini, K., Vaara, M., Huang, D., Pospiech, H., Majuri, I., Westerling, T., Makela, T. P., Syvaoja, J. E. (2001). BRCT Domain-containing Protein TopBP1 Functions in DNA Replication and Damage Response. J. Biol. Chem. 276: 30399-30406 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»