This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mittelmeier, T M Articles by Dieckmann, C L Search for Related Content PubMed PubMed Citation Articles by Mittelmeier, T M Articles by Dieckmann, C L

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 July; 13(7): 4203-4213

In vivo analysis of sequences necessary for CBP1-dependent accumulation of cytochrome b transcripts in yeast mitochondria.

Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721.

ABSTRACT

In Saccharomyces cerevisiae, cytochrome b, an essential component of the respiratory chain, is encoded by the mitochondrial gene cob. The cob transcription unit includes the tRNA(Glu) gene from positions -1170 to -1099 relative to the cob ATG at +1. The initial tRNA(Glu)-cob transcript undergoes several processing events, including removal of tRNA(Glu) and production of the mature 5' end of cob mRNA at nucleotide -954. The nuclear gene product CBP1 is specifically required for the accumulation of cob mRNA. In cbp1 mutant strains, cob transcripts are not detectable by Northern (RNA) blot analysis, but the steady-state level of tRNA(Glu) is similar to that of wild type. The results of a previous study led to the conclusion that a 400-nucleotide region just downstream of tRNA(Glu) is sufficient for CBP1 function. In the present study, the microprojectile bombardment method of mitochondrial transformation was used to introduce deletions within this region of cob. The analysis of cob transcripts in strains carrying the mitochondrial deletion genomes indicates that a 63-nucleotide sequence that encompasses the cleavage site at -954 is sufficient both for CBP1 function and for correct positioning of the cleavage. Furthermore, the data indicate that CBP1 prevents the degradation of unprocessed cob transcripts produced by endonucleolytic cleavage at the 3' end of tRNA(Glu).

This article has been cited by other articles:

• Fekete, Z., Ellis, T. P., Schonauer, M. S., Dieckmann, C. L. (2008). Pet127 Governs a 5' -> 3'-Exonuclease Important in Maturation of Apocytochrome b mRNA in Saccharomyces cerevisiae. J. Biol. Chem. 283: 3767-3772 [Abstract] [Full Text]  
• Ellis, T. P., Schonauer, M. S., Dieckmann, C. L. (2005). CBT1 Interacts Genetically With CBP1 and the Mitochondrially Encoded Cytochrome b Gene and Is Required to Stabilize the Mature Cytochrome b mRNA of Saccharomyces cerevisiae. Genetics 171: 949-957 [Abstract] [Full Text]  
• Erickson, B., Stern, D. B., Higgs, D. C. (2005). Microarray Analysis Confirms the Specificity of a Chlamydomonas reinhardtii Chloroplast RNA Stability Mutant. Plant Physiol. 137: 534-544 [Abstract] [Full Text]  
• Krause, K., Dieckmann, C. L. (2004). Analysis of transcription asymmetries along the tRNAE-COB operon: evidence for transcription attenuation and rapid RNA degradation between coding sequences. Nucleic Acids Res 32: 6276-6283 [Abstract] [Full Text]  
• Krause, K., Lopes de Souza, R., Roberts, D. G.W., Dieckmann, C. L. (2004). The Mitochondrial Message-specific mRNA Protectors Cbp1 and Pet309 Are Associated in a High-Molecular Weight Complex. Mol. Biol. Cell 15: 2674-2683 [Abstract] [Full Text]  
• Ellis, T. P., Helfenbein, K. G., Tzagoloff, A., Dieckmann, C. L. (2004). Aep3p Stabilizes the Mitochondrial Bicistronic mRNA Encoding Subunits 6 and 8 of the H+-translocating ATP Synthase of Saccharomyces cerevisiae. J. Biol. Chem. 279: 15728-15733 [Abstract] [Full Text]  
• Islas-Osuna, M. A., Ellis, T. P., Marnell, L. L., Mittelmeier, T. M., Dieckmann, C. L. (2002). Cbp1 Is Required for Translation of the Mitochondrial Cytochrome b mRNA of Saccharomyces cerevisiae. J. Biol. Chem. 277: 37987-37990 [Abstract] [Full Text]  
• Higgs, D. C., Shapiro, R. S., Kindle, K. L., Stern, D. B. (1999). Small cis-Acting Sequences That Specify Secondary Structures in a Chloroplast mRNA Are Essential for RNA Stability and Translation. Mol. Cell. Biol. 19: 8479-8491 [Abstract] [Full Text]  
• Lupold, D. S., Caoile, A. G. F. S., Stern, D. B. (1999). Genomic context influences the activity of maize mitochondrial cox2 promoters. Proc. Natl. Acad. Sci. USA 96: 11670-11675 [Abstract] [Full Text]  
• Chen, W., Islas-Osuna, M. A., Dieckmann, C. L. (1999). Suppressor Analysis of Mutations in the 5'-Untranslated Region of COB mRNA Identifies Components of General Pathways for Mitochondrial mRNA Processing and Decay in Saccharomyces cerevisiae. Genetics 151: 1315-1325 [Abstract] [Full Text]