This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Perry, J R
Right arrow Articles by Becker, J M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Perry, J R
Right arrow Articles by Becker, J M

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 January; 14(1): 104-115

Isolation and characterization of a Saccharomyces cerevisiae peptide transport gene.

J R Perry, M A Basrai, H Y Steiner, F Naider and J M Becker

Department of Microbiology, University of Tennessee, Knoxville 37996.

ABSTRACT

We have cloned and characterized a Saccharomyces cerevisiae peptide transport gene (PTR2) isolated from a genomic DNA library by directly selecting for functional complementation of a peptide transport-deficient mutant. Deletion and frameshift mutageneses were used to localize the complementing activity to a 3.1-kbp region on the transforming plasmid. DNA sequencing of the complementing region identified an open reading frame spanning 1,803 bp. The deduced amino acid sequence predicts a hydrophobic peptide consisting of 601 amino acids, having a molecular mass of 68.1 kDa, composed in part of 12 hydrophobic segments, and sharing significant similarities with a nitrate transport protein encoded by the CHL1 gene of Arabidopsis thaliana. Northern (RNA) hybridization experiments demonstrated a single transcript that was 1.8 kb in length and that was transiently induced by the addition of L-leucine to the growth medium. The PTR2 gene was localized to the right arm of chromosome XI by contour-clamped homogeneous electric field gel chromosome blotting and by hybridization to known chromosome XI lambda phage clones of S. cerevisiae DNA. PTR2 was tightly linked to the UBI2 gene, with the coding sequences being separated by a 466-bp region and oriented so that the genes were transcribed convergently. A chromosomal disruption of the PTR2 gene in a haploid strain was not lethal under standard growth conditions. The cloning of PTR2 represents the first example of the molecular genetic characterization of a eucaryotic peptide transport gene.

Mol Cell Biol. 1994 January; 14(1): 104-115




This article has been cited by other articles:

  • Wakiec, R., Gabriel, I., Prasad, R., Becker, J. M., Payne, J. W., Milewski, S. (2008). Enhanced Susceptibility to Antifungal Oligopeptides in Yeast Strains Overexpressing ABC Multidrug Efflux Pumps. Antimicrob. Agents Chemother. 52: 4057-4063 [Abstract] [Full Text]  
  • Cai, H., Hauser, M., Naider, F., Becker, J. M. (2007). Differential Regulation and Substrate Preferences in Two Peptide Transporters of Saccharomyces cerevisiae. Eukaryot Cell 6: 1805-1813 [Abstract] [Full Text]  
  • Wiles, A. M., Cai, H., Naider, F., Becker, J. M. (2006). Nutrient regulation of oligopeptide transport in Saccharomyces cerevisiae.. Microbiology 152: 3133-3145 [Abstract] [Full Text]  
  • Daniel, H., Spanier, B., Kottra, G., Weitz, D. (2006). From Bacteria to Man: Archaic Proton-Dependent Peptide Transporters at Work. Physiology 21: 93-102 [Abstract] [Full Text]  
  • Cai, H., Kauffman, S., Naider, F., Becker, J. M. (2006). Genomewide Screen Reveals a Wide Regulatory Network for Di/Tripeptide Utilization in Saccharomyces cerevisiae. Genetics 172: 1459-1476 [Abstract] [Full Text]  
  • Eckert-Boulet, N., Larsson, K., Wu, B., Poulsen, P., Regenberg, B., Nielsen, J., Kielland-Brandt, M. C. (2006). Deletion of RTS1, Encoding a Regulatory Subunit of Protein Phosphatase 2A, Results in Constitutive Amino Acid Signaling via Increased Stp1p Processing. Eukaryot Cell 5: 174-179 [Abstract] [Full Text]  
  • Chiang, C.-S., Stacey, G., Tsay, Y.-F. (2004). Mechanisms and Functional Properties of Two Peptide Transporters, AtPTR2 and fPTR2. J. Biol. Chem. 279: 30150-30157 [Abstract] [Full Text]  
  • Daniel, H., Rubio-Aliaga, I. (2003). An update on renal peptide transporters. Am. J. Physiol. Renal Physiol. 284: F885-F892 [Abstract] [Full Text]  
  • Blyszczuk, P., Czyz, J., Kania, G., Wagner, M., Roll, U., St-Onge, L., Wobus, A. M. (2003). Expression of Pax4 in embryonic stem cells promotes differentiation of nestin-positive progenitor and insulin-producing cells. Proc. Natl. Acad. Sci. USA 100: 998-1003 [Abstract] [Full Text]  
  • Stacey, M. G., Koh, S., Becker, J., Stacey, G. (2002). AtOPT3, a Member of the Oligopeptide Transporter Family, Is Essential for Embryo Development in Arabidopsis. Plant Cell 14: 2799-2811 [Abstract] [Full Text]  
  • Forsberg, H., Ljungdahl, P. O. (2001). Genetic and Biochemical Analysis of the Yeast Plasma Membrane Ssy1p-Ptr3p-Ssy5p Sensor of Extracellular Amino Acids. Mol. Cell. Biol. 21: 814-826 [Abstract] [Full Text]  
  • Mignini, F., Prasad, R., Borowski, E. (2001). Unusual Susceptibility of a Multidrug-Resistant Yeast Strain to Peptidic Antifungals. Antimicrob. Agents Chemother. 45: 223-228 [Abstract] [Full Text]  
  • Fang, G., Konings, W. N., Poolman, B. (2000). Kinetics and Substrate Specificity of Membrane-Reconstituted Peptide Transporter DtpT of Lactococcus lactis. J. Bacteriol. 182: 2530-2535 [Abstract] [Full Text]  
  • Bourbouloux, A., Shahi, P., Chakladar, A., Delrot, S., Bachhawat, A. K. (2000). Hgt1p, a High Affinity Glutathione Transporter from the Yeast Saccharomyces cerevisiae. J. Biol. Chem. 275: 13259-13265 [Abstract] [Full Text]  
  • Lin, C.-M., Koh, S., Stacey, G., Yu, S.-M., Lin, T.-Y., Tsay, Y.-F. (2000). Cloning and Functional Characterization of a Constitutively Expressed Nitrate Transporter Gene, OsNRT1, from Rice. Plant Physiol. 122: 379-388 [Abstract] [Full Text]  
  • Klasson, H., Fink, G. R., Ljungdahl, P. O. (1999). Ssy1p and Ptr3p Are Plasma Membrane Components of a Yeast System That Senses Extracellular Amino Acids. Mol. Cell. Biol. 19: 5405-5416 [Abstract] [Full Text]  
  • Iraqui, I., Vissers, S., Bernard, F., de Craene, J.-O., Boles, E., Urrestarazu, A., Andre, B. (1999). Amino Acid Signaling in Saccharomyces cerevisiae: a Permease-Like Sensor of External Amino Acids and F-Box Protein Grr1p Are Required for Transcriptional Induction of the AGP1 Gene, Which Encodes a Broad-Specificity Amino Acid Permease. Mol. Cell. Biol. 19: 989-1001 [Abstract] [Full Text]  
  • Roman, G., Meller, V., Wu, K. H., Davis, R. L. (1998). The opt1 gene of Drosophila melanogaster encodes a proton-dependent dipeptide transporter. Am. J. Physiol. Cell Physiol. 275: C857-C869 [Abstract] [Full Text]  
  • Yamashita, T., Shimada, S., Guo, W., Sato, K., Kohmura, E., Hayakawa, T., Takagi, T., Tohyama, M. (1997). Cloning and Functional Expression of a Brain Peptide/Histidine Transporter. J. Biol. Chem. 272: 10205-10211 [Abstract] [Full Text]  
  • Beauvais, A., Monod, M., Debeaupuis, J.-P., Diaquin, M., Kobayashi, H., Latge, J.-P. (1997). Biochemical and Antigenic Characterization of a New Dipeptidyl-Peptidase Isolated from Aspergillus fumigatus. J. Biol. Chem. 272: 6238-6244 [Abstract] [Full Text]  
  • Liang, R., Fei, Y.-J., Prasad, P. D., Ramamoorthy, S., Han, H., Yang-Feng, T. L., Hediger, M. A., Ganapathy, V., Leibach, F. H. (1995). Human Intestinal H[IMAGE]/Peptide Cotransporter. J. Biol. Chem. 270: 6456-6463 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»