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Mol. Cell. Biol., Jan 1995, 173-178, Vol 15, No. 1
Copyright © 1995, American Society for Microbiology

DNA synthesis generally initiates outside the simian virus 40 core origin in vitro

PA Bullock and D Denis
Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts 02111.

The nucleotide positions at which DNA synthesis initiates in vitro, in the vicinity of the simian virus 40 origin, have been determined. Start sites for DNA synthesis are greatly suppressed over the simian virus 40 core origin. Relatively weak start sites are detected over the 21-bp repeats and T-antigen-binding site I; distal to these regions, stronger start sites are detected. Thus, studies using a model system for eukaryotic DNA replication indicate that DNA synthesis events initiate, in general, outside the core origin.

This article has been cited by other articles:

• Purviance, J. D., Prack, A. E., Barbaro, B., Bullock, P. A. (2001). In the Simian Virus 40 In Vitro Replication System, Start Site Selection by the Polymerase {alpha}-Primase Complex Is Not Significantly Altered by Changes in the Concentration of Ribonucleotides. J. Virol. 75: 6392-6401 [Abstract] [Full Text]  
• Weisshart, K., Taneja, P., Fanning, E. (1998). The Replication Protein A Binding Site in Simian Virus 40 (SV40) T Antigen and Its Role in the Initial Steps of SV40 DNA Replication. J. Virol. 72: 9771-9781 [Abstract] [Full Text]