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Mol. Cell. Biol., Jan 1995, 186-197, Vol 15, No. 1
Copyright © 1995, American Society for Microbiology

Association of p62, a multifunctional SH2- and SH3-domain-binding protein, with src family tyrosine kinases, Grb2, and phospholipase C gamma-1

S Richard, D Yu, KJ Blumer, D Hausladen, MW Olszowy, PA Connelly and AS Shaw
Center for Immunology, Washington University School of Medicine, St Louis, Missouri 63110.

src family tyrosine kinases contain two noncatalytic domains termed src homology 3 (SH3) and SH2 domains. Although several other signal transduction molecules also contain tandemly occurring SH3 and SH2 domains, the function of these closely spaced domains is not well understood. To identify the role of the SH3 domains of src family tyrosine kinases, we sought to identify proteins that interacted with this domain. By using the yeast two-hybrid system, we identified p62, a tyrosine-phosphorylated protein that associates with p21ras GTPase- activating protein, as a src family kinase SH3-domain-binding protein. Reconstitution of complexes containing p62 and the src family kinase p59fyn in HeLa cells demonstrated that complex formation resulted in tyrosine phosphorylation of p62 and was mediated by both the SH3 and SH2 domains of p59fyn. The phosphorylation of p62 by p59fyn required an intact SH3 domain, demonstrating that one function of the src family kinase SH3 domains is to bind and present certain substrates to the kinase. As p62 contains at least five SH3-domain-binding motifs and multiple tyrosine phosphorylation sites, p62 may interact with other signalling molecules via SH3 and SH2 domain interactions. Here we show that the SH3 and/or SH2 domains of the signalling proteins Grb2 and phospholipase C gamma-1 can interact with p62 both in vitro and in vivo. Thus, we propose that one function of the tandemly occurring SH3 and SH2 domains of src family kinases is to bind p62, a multifunctional SH3 and SH2 domain adapter protein, linking src family kinases to downstream effector and regulatory molecules.


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  • Kanemitsu, M. Y., Loo, L. W. M., Simon, S., Lau, A. F., Eckhart, W. (1997). Tyrosine Phosphorylation of Connexin 43 by v-Src Is Mediated by SH2 and SH3 Domain Interactions. J. Biol. Chem. 272: 22824-22831 [Abstract] [Full Text]  
  • Fusaki, N., Iwamatsu, A., Iwashima, M., Fujisawa, J.-i. (1997). Interaction between Sam68 and Src Family Tyrosine Kinases, Fyn and Lck, in T Cell Receptor Signaling. J. Biol. Chem. 272: 6214-6219 [Abstract] [Full Text]  
  • Barlat, I., Maurier, F., Duchesne, M., Guitard, E., Tocque, B., Schweighoffer, F. (1997). A Role for Sam68 in Cell Cycle Progression Antagonized by a Spliced Variant within the KH Domain. J. Biol. Chem. 272: 3129-3132 [Abstract] [Full Text]  
  • Trub, T., Frantz, J. D., Miyazaki, M., Band, H., Shoelson, S. E. (1997). The Role of a Lymphoid-restricted, Grb2-like SH3-SH2-SH3 Protein in T Cell Receptor Signaling. J. Biol. Chem. 272: 894-902 [Abstract] [Full Text]  
  • Baehrecke, E. (1997). who encodes a KH RNA binding protein that functions in muscle development. Development 124: 1323-1332 [Abstract]  
  • Bunnell, S. C., Henry, P. A., Kolluri, R., Kirchhausen, T., Rickles, R. J., Berg, L. J. (1996). Identification of Itk/Tsk Src Homology 3Domain Ligands. J. Biol. Chem. 271: 25646-25656 [Abstract] [Full Text]  
  • Beslu, N., LaRose, J., Casteran, N., Birnbaum, D., Lecocq, E., Dubreuil, P., Rottapel, R. (1996). Phosphatidylinositol-3' Kinase Is Not Required for Mitogenesis or Internalization of the Flt3/Flk2 Receptor Tyrosine Kinase. J. Biol. Chem. 271: 20075-20081 [Abstract] [Full Text]  
  • Takahashi, F, Endo, S, Kojima, T, Saigo, K (1996). Regulation of cell-cell contacts in developing Drosophila eyes by Dsrc41, a new, close relative of vertebrate c-src.. Genes Dev. 10: 1645-1656 [Abstract]  
  • Alexandropoulos, K, Baltimore, D (1996). Coordinate activation of c-Src by SH3- and SH2-binding sites on a novel p130Cas-related protein, Sin.. Genes Dev. 10: 1341-1355 [Abstract]  
  • Mukai, H., Toshimori, M., Shibata, H., Kitagawa, M., Shimakawa, M., Miyahara, M., Sunakawa, H., Ono, Y. (1996). PKN Associates and Phosphorylates the Head-Rod Domain of Neurofilament Protein. J. Biol. Chem. 271: 9816-9822 [Abstract] [Full Text]  
  • Nakamoto, T., Sakai, R., Ozawa, K., Yazaki, Y., Hirai, H. (1996). Direct Binding of C-terminal Region of p130[IMAGE] to SH2 and SH3 Domains of Src Kinase. J. Biol. Chem. 271: 8959-8965 [Abstract] [Full Text]  
  • Nunès, J. A., Truneh, A., Olive, D., Cantrell, D. A. (1996). Signal Transduction by CD28 Costimulatory Receptor on T Cells. J. Biol. Chem. 271: 1591-1598 [Abstract] [Full Text]  
  • Hanke, J. H., Gardner, J. P., Dow, R. L., Changelian, P. S., Brissette, W. H., Weringer, E. J., Pollok, B. A., Connelly, P. A. (1996). Discovery of a Novel, Potent, and Src Family-selective Tyrosine Kinase Inhibitor. J. Biol. Chem. 271: 695-701 [Abstract] [Full Text]  
  • Van Seuningen, I., Ostrowski, J., Bustelo, X. R., Sleath, P. R., Bomsztyk, K. (1995). The K Protein Domain That Recruits the Interleukin 1-responsive K Protein Kinase Lies Adjacent to a Cluster of c-Src and Vav SH3-binding Sites. J. Biol. Chem. 270: 26976-26985 [Abstract] [Full Text]  
  • Fukazawa, T., Reedquist, K. A., Trub, T., Soltoff, S., Panchamoorthy, G., Druker, B., Cantley, L., Shoelson, S. E., Band, H. (1995). The SH3 Domain-binding T Cell Tyrosyl Phosphoprotein p120. J. Biol. Chem. 270: 19141-19150 [Abstract] [Full Text]  
  • Jones, A R, Schedl, T (1995). Mutations in gld-1, a female germ cell-specific tumor suppressor gene in Caenorhabditis elegans, affect a conserved domain also found in Src-associated protein Sam68.. Genes Dev. 9: 1491-1504 [Abstract]  
  • Taylor, S. J., Anafi, M., Pawson, T., Shalloway, D. (1995). Functional Interaction between c-Src and Its Mitotic Target, Sam 68. J. Biol. Chem. 270: 10120-10124 [Abstract] [Full Text]  
  • Wang, L. L., Richard, S.ép., Shaw, A. S. (1995). p62 Association with RNA Is Regulated by Tyrosine Phosphorylation. J. Biol. Chem. 270: 2010-2013 [Abstract] [Full Text]  
  • Hresko, R. C., Mueckler, M. (2000). A Novel 68-kDa Adipocyte Protein Phosphorylated on Tyrosine in Response to Insulin and Osmotic Shock. J. Biol. Chem. 275: 18114-18120 [Abstract] [Full Text]  
  • Edwards, J. C., Kapadia, S. (2000). Regulation of the Bovine Kidney Microsomal Chloride Channel p64 by p59fyn, a Src Family Tyrosine Kinase. J. Biol. Chem. 275: 31826-31832 [Abstract] [Full Text]  
  • Liu, K., Li, L., Nisson, P. E., Gruber, C., Jessee, J., Cohen, S. N. (2000). Neoplastic Transformation and Tumorigenesis Associated with Sam68 Protein Deficiency in Cultured Murine Fibroblasts. J. Biol. Chem. 275: 40195-40201 [Abstract] [Full Text]  
  • Stoss, O., Olbrich, M., Hartmann, A. M., Konig, H., Memmott, J., Andreadis, A., Stamm, S. (2001). The STAR/GSG Family Protein rSLM-2 Regulates the Selection of Alternative Splice Sites. J. Biol. Chem. 276: 8665-8673 [Abstract] [Full Text]  
  • Arold, S. T., Ulmer, T. S., Mulhern, T. D., Werner, J. M., Ladbury, J. E., Campbell, I. D., Noble, M. E. M. (2001). The Role of the Src Homology 3-Src Homology 2 Interface in the Regulation of Src Kinases. J. Biol. Chem. 276: 17199-17205 [Abstract] [Full Text]  
  • Chen, T., Cote, J., Carvajal, H. V., Richard, S. (2001). Identification of Sam68 Arginine Glycine-rich Sequences Capable of Conferring Nonspecific RNA Binding to the GSG Domain. J. Biol. Chem. 276: 30803-30811 [Abstract] [Full Text]  
  • Bedford, M. T., Frankel, A., Yaffe, M. B., Clarke, S., Leder, P., Richard, S. (2000). Arginine Methylation Inhibits the Binding of Proline-rich Ligands to Src Homology 3, but Not WW, Domains. J. Biol. Chem. 275: 16030-16036 [Abstract] [Full Text]  


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