This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pande, S.
Right arrow Articles by Farabaugh, P. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pande, S.
Right arrow Articles by Farabaugh, P. J.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Jan 1995, 298-304, Vol 15, No. 1
Copyright © 1995, American Society for Microbiology

Pulling the ribosome out of frame by +1 at a programmed frameshift site by cognate binding of aminoacyl-tRNA

S Pande, A Vimaladithan, H Zhao and PJ Farabaugh
Department of Biological Sciences, University of Maryland, Baltimore 21228.

Programmed translational frameshifts efficiently alter a translational reading frame by shifting the reading frame during translation. A +1 frameshift has two simultaneous requirements: a translational pause which occurs when either an inefficiently recognized sense or termination codon occupies the A site, and the presence of a special peptidyl-tRNA occupying the P site during the pause. The special nature of the peptidyl-tRNA reflects its ability to slip +1 on the mRNA or to facilitate binding of an incoming aminoacyl-tRNA out of frame in the A site. This second mechanism suggested that in some cases the first +1 frame tRNA could have an active role in frameshifting. We found that overproducing this tRNA can drive frameshifting, surprisingly regardless of whether frameshifting occurs by peptidyl-tRNA slippage or out-of-frame binding of aminoacyl-tRNA. This finding suggests that in both cases, the shift in reading frame occurs coincident with formation of a cognate codon-anticodon interaction in the shifted frame.


This article has been cited by other articles:

  • Liao, P.-Y., Choi, Y. S., Lee, K. H. (2009). FSscan: a mechanism-based program to identify +1 ribosomal frameshift hotspots. Nucleic Acids Res 0: gkp796v1-gkp796 [Abstract] [Full Text]  
  • Vallabhaneni, H., Fan-Minogue, H., Bedwell, D. M., Farabaugh, P. J. (2009). Connection between stop codon reassignment and frequent use of shifty stop frameshifting. RNA 15: 889-897 [Abstract] [Full Text]  
  • Wong, T.-Y., Fernandes, S., Sankhon, N., Leong, P. P., Kuo, J., Liu, J.-K. (2008). Role of Premature Stop Codons in Bacterial Evolution. J. Bacteriol. 190: 6718-6725 [Abstract] [Full Text]  
  • Guarraia, C., Norris, L., Raman, A., Farabaugh, P. J. (2007). Saturation mutagenesis of a +1 programmed frameshift-inducing mRNA sequence derived from a yeast retrotransposon. RNA 13: 1940-1947 [Abstract] [Full Text]  
  • Henderson, C. M., Anderson, C. B., Howard, M. T. (2006). Antisense-induced ribosomal frameshifting. Nucleic Acids Res 34: 4302-4310 [Abstract] [Full Text]  
  • IVANOV, I. P., GESTELAND, R. F., ATKINS, J. F. (2006). Evolutionary specialization of recoding: Frameshifting in the expression of S. cerevisiae antizyme mRNA is via an atypical antizyme shift site but is still +1. RNA 12: 332-337 [Abstract] [Full Text]  
  • Gurvich, O. L., Baranov, P. V., Gesteland, R. F., Atkins, J. F. (2005). Expression Levels Influence Ribosomal Frameshifting at the Tandem Rare Arginine Codons AGG_AGG and AGA_AGA in Escherichia coli. J. Bacteriol. 187: 4023-4032 [Abstract] [Full Text]  
  • BARANOV, P. V., GESTELAND, R. F., ATKINS, J. F. (2004). P-site tRNA is a crucial initiator of ribosomal frameshifting. RNA 10: 221-230 [Abstract] [Full Text]  
  • GAO, X., HAVECKER, E. R., BARANOV, P. V., ATKINS, J. F., VOYTAS, D. F. (2003). Translational recoding signals between gag and pol in diverse LTR retrotransposons. RNA 9: 1422-1430 [Abstract] [Full Text]  
  • FUKUNISHI, Y., HAYASHIZAKI, Y. (2001). Amino acid translation program for full-length cDNA sequences with frameshift errors. Physiol. Genomics 5: 81-87 [Abstract] [Full Text]  
  • STAHL, G., BEN SALEM, S., LI, Z., MCCARTY, G., RAMAN, A., SHAH, M., FARABAUGH, P.J. (2001). Programmed +1 Translational Frameshifting in the Yeast Saccharomyces cerevisiae Results from Disruption of Translational Error Correction. Cold Spring Harb Symp Quant Biol 66: 249-258 [Abstract]  
  • McCarthy, J. E. G. (1998). Posttranscriptional Control of Gene Expression in Yeast. Microbiol. Mol. Biol. Rev. 62: 1492-1553 [Abstract] [Full Text]  
  • Farabaugh, P. J., Farabaugh, P. J. (1995). Post-transcriptional Regulation of Transposition by Ty Retrotransposons of Saccharomyces cerevisiae. J. Biol. Chem. 270: 10361-10364 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»