This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gopal-Srivastava, R.
Right arrow Articles by Piatigorsky, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gopal-Srivastava, R.
Right arrow Articles by Piatigorsky, J.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 12 1995, 7081-7090, Vol 15, No. 12
Copyright © 1995, American Society for Microbiology

Regulation of the murine alpha B-crystallin/small heat shock protein gene in cardiac muscle

R Gopal-Srivastava, JI Haynes 2nd and J Piatigorsky
Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, Maryland 20892-2730, USA.

The murine alpha B-crystallin/small heat shock protein gene is expressed at high levels in the lens and at lower levels in the heart, skeletal muscle, and numerous other tissues. Previously we have found a skeletal-muscle-preferred enhancer at positions -427 to -259 of the alpha B-crystallin gene containing at least four cis-acting regulatory elements (alpha BE-1, alpha BE-2, alpha BE-3, and MRF, which has an E box). Here we show that in transgenic mice, the alpha B-crystallin enhancer directs the chloramphenicol acetyltransferase reporter gene driven by the alpha B-crystallin promoter specifically to myocardiocytes of the heart. The alpha B-crystallin enhancer was active in conjugation with the herpes simplex virus thymidine kinase promoter/human growth hormone reporter gene in transfected rat myocardiocytes. DNase I footprinting and site-specific mutagenesis experiments showed that alpha BE-1, alpha BE-2, alpha BE-3, MRF, and a novel, heart-specific element called alpha BE-4 are required for alpha B-crystallin enhancer activity in transfected myocardiocytes. By contrast, alpha BE-4 is not utilized for enhancer activity in transfected lens or skeletal muscle cell lines. Alpha BE-4 contains an overlapping heat shock sequence and a reverse CArG box [5'-GG(A/T)6CC- 3']. Electrophoretic mobility shift assays with an antibody to serum response factor and a CArG-box-competing sequence from the c-fos promoter indicated that a cardiac-specific protein with DNA-binding and antigenic similarities to serum response factor binds to alpha BE-4 via the reverse CArG box; electrophoretic mobility shift assays and antibody experiments with anti-USF antiserum and heart nuclear extract also raised the possibility that the MRF E box utilizes USF or an antigenically related protein. We conclude that the activity of the alpha B-crystallin enhancer in the heart utilizes a reverse CArG box and an E-box-dependent pathway.


This article has been cited by other articles:

  • Li, Y., Hough, R. B., Piatigorsky, J. (2007). Tissue-specific activity of the blind mole rat and the two nucleotide-mutated mouse {alpha}B-crystallin promoter in transgenic mice. Proc. Natl. Acad. Sci. USA 104: 2608-2613 [Abstract] [Full Text]  
  • Mathew, S., Mascareno, E., Siddiqui, M. A. Q. (2004). A Ternary Complex of Transcription Factors, Nished and NFATc4, and Co-activator p300 Bound to an Intronic Sequence, Intronic Regulatory Element, Is Pivotal for the Up-regulation of Myosin Light Chain-2v Gene in Cardiac Hypertrophy. J. Biol. Chem. 279: 41018-41027 [Abstract] [Full Text]  
  • Zhao, H., Yang, Y., Rizo, C. M., Overbeek, P. A., Robinson, M. L. (2004). Insertion of a Pax6 Consensus Binding Site into the {alpha}A-Crystallin Promoter Acts as a Lens Epithelial Cell Enhancer in Transgenic Mice. IOVS 45: 1930-1939 [Abstract] [Full Text]  
  • Morrison, L. E., Whittaker, R. J., Klepper, R. E., Wawrousek, E. F., Glembotski, C. C. (2004). Roles for {alpha}B-crystallin and HSPB2 in protecting the myocardium from ischemia-reperfusion-induced damage in a KO mouse model. Am. J. Physiol. Heart Circ. Physiol. 286: H847-H855 [Abstract] [Full Text]  
  • Moore, M. L., Park, E. A., McMillin, J. B. (2003). Upstream Stimulatory Factor Represses the Induction of Carnitine Palmitoyltransferase-Ibeta Expression by PGC-1. J. Biol. Chem. 278: 17263-17268 [Abstract] [Full Text]  
  • Swamynathan, S. K., Piatigorsky, J. (2002). Orientation-dependent Influence of an Intergenic Enhancer on the Promoter Activity of the Divergently Transcribed Mouse Shsp/alpha B-crystallin and Mkbp/HspB2 Genes. J. Biol. Chem. 277: 49700-49706 [Abstract] [Full Text]  
  • Hough, R. B., Avivi, A., Davis, J., Joel, A., Nevo, E., Piatigorsky, J. (2002). Adaptive evolution of small heat shock protein/ alpha B-crystallin promoter activity of the blind subterranean mole rat, Spalax ehrenbergi. Proc. Natl. Acad. Sci. USA 99: 8145-8150 [Abstract] [Full Text]  
  • Sugiyama, Y., Suzuki, A., Kishikawa, M., Akutsu, R., Hirose, T., Waye, M. M. Y., Tsui, S. K. W., Yoshida, S., Ohno, S. (2000). Muscle Develops a Specific Form of Small Heat Shock Protein Complex Composed of MKBP/HSPB2 and HSPB3 during Myogenic Differentiation. J. Biol. Chem. 275: 1095-1104 [Abstract] [Full Text]  
  • Benjamin, I. J., McMillan, D. R. (1998). Stress (Heat Shock) Proteins : Molecular Chaperones in Cardiovascular Biology and Disease. Circ. Res. 83: 117-132 [Abstract] [Full Text]  
  • Gopal-Srivastava, R., Cvekl, A., Piatigorsky, J. (1998). Involvement of Retinoic Acid/Retinoid Receptors in the Regulation of Murine alpha B-crystallin/Small Heat Shock Protein Gene Expression in the Lens. J. Biol. Chem. 273: 17954-17961 [Abstract] [Full Text]  
  • Liang, P., Amons, R., Clegg, J. S., MacRae, T. H. (1997). Molecular Characterization of a Small Heat Shock/alpha -Crystallin Protein in Encysted Artemia Embryos. J. Biol. Chem. 272: 19051-19058 [Abstract] [Full Text]  
  • Gopal-Srivastava, R., Cvekl, A., Piatigorsky, J. (1996). Pax-6 and alpha B-crystallin/Small Heat Shock Protein Gene Regulation in the Murine Lens. INTERACTION WITH THE LENS-SPECIFIC REGIONS, LSR1 AND LSR2. J. Biol. Chem. 271: 23029-23036 [Abstract] [Full Text]  
  • Hoover, H. E., Thuerauf, D. J., Martindale, J. J., Glembotski, C. C. (2000). alpha B-crystallin Gene Induction and Phosphorylation by MKK6-activated p38. A POTENTIAL ROLE FOR alpha B-CRYSTALLIN AS A TARGET OF THE p38 BRANCH OF THE CARDIAC STRESS RESPONSE. J. Biol. Chem. 275: 23825-23833 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»