This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peers, B.
Right arrow Articles by Monteminy, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peers, B.
Right arrow Articles by Monteminy, M.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Dec 1995, 7091-7097, Vol 15, No. 12
Copyright © 1995, American Society for Microbiology

The pancreatic islet factor STF-1 binds cooperatively with Pbx to a regulatory element in the somatostatin promoter: importance of the FPWMK motif and of the homeodomain

B Peers, S Sharma, T Johnson, M Kamps and M Monteminy
Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, California 92037, USA.

A number of homeodomain proteins have been shown to regulate cellular development by stimulating the transcription of specific target genes. In contrast to their distinct activities in vivo, however, most homeodomain proteins bind indiscriminately to potential target sites in vitro, suggesting the involvement of cofactors which specify target site selection. One such cofactor, termed extradenticle, has been shown to influence segmental morphogenesis in Drosophila melanogaster by binding cooperatively with certain homeodomain proteins to target regulatory elements. Here we demonstrate that STF-1, an orphan homeodomain protein required for pancreatic development in mammals, binds cooperatively to DNA with Pbx, the mammalian homolog of extradenticle. Cooperative binding with Pbx requires a pentapeptide motif (FPWMK) which is well conserved among a large subset of homeodomain proteins. The FPMWK motif is not sufficient to confer Pbx cooperativity on other homeodomain proteins, however; the N-terminal arm of the STF-1 homeodomain is also essential. As cooperative binding with Pbx occurs on only a subset of potential STF-1 target sites, our results suggest that Pbx may specify target gene selection in the developing pancreas by forming heterodimeric complexes with STF-1.


This article has been cited by other articles:

  • Boucher, M.-J., Simoneau, M., Edlund, H. (2009). The Homeodomain-Interacting Protein Kinase 2 Regulates Insulin Promoter Factor-1/Pancreatic Duodenal Homeobox-1 Transcriptional Activity. Endocrinology 150: 87-97 [Abstract] [Full Text]  
  • Andronis, C., Barak, S., Knowles, S. M., Sugano, S., Tobin, E. M. (2008). The Clock Protein CCA1 and the bZIP Transcription Factor HY5 Physically Interact to Regulate Gene Expression in Arabidopsis. Mol Plant 1: 58-67 [Abstract] [Full Text]  
  • Maves, L., Waskiewicz, A. J., Paul, B., Cao, Y., Tyler, A., Moens, C. B., Tapscott, S. J. (2007). Pbx homeodomain proteins direct Myod activity to promote fast-muscle differentiation. Development 134: 3371-3382 [Abstract] [Full Text]  
  • Gromada, J., Franklin, I., Wollheim, C. B. (2007). {alpha}-Cells of the Endocrine Pancreas: 35 Years of Research but the Enigma Remains. Endocr. Rev. 28: 84-116 [Abstract] [Full Text]  
  • Deramaudt, T. B., Sachdeva, M. M., Wescott, M. P., Chen, Y., Stoffers, D. A., Rustgi, A. K. (2006). The PDX1 Homeodomain Transcription Factor Negatively Regulates the Pancreatic Ductal Cell-specific Keratin 19 Promoter. J. Biol. Chem. 281: 38385-38395 [Abstract] [Full Text]  
  • Lebrun, P., Montminy, M. R., Van Obberghen, E. (2005). Regulation of the Pancreatic Duodenal Homeobox-1 Protein by DNA-dependent Protein Kinase. J. Biol. Chem. 280: 38203-38210 [Abstract] [Full Text]  
  • Arca, B., Lombardo, F., Valenzuela, J. G., Francischetti, I. M. B., Marinotti, O., Coluzzi, M., Ribeiro, J. M. C. (2005). An updated catalogue of salivary gland transcripts in the adult female mosquito, Anopheles gambiae. J. Exp. Biol. 208: 3971-3986 [Abstract] [Full Text]  
  • Pino, M. F., Ye, D. Z., Linning, K. D., Green, C. D., Wicksteed, B., Poitout, V., Olson, L. K. (2005). Elevated Glucose Attenuates Human Insulin Gene Promoter Activity in INS-1 Pancreatic {beta}-Cells via Reduced Nuclear Factor Binding to the A5/Core and Z Element. Mol. Endocrinol. 19: 1343-1360 [Abstract] [Full Text]  
  • Selleri, L., DiMartino, J., van Deursen, J., Brendolan, A., Sanyal, M., Boon, E., Capellini, T., Smith, K. S., Rhee, J., Popperl, H., Grosveld, G., Cleary, M. L. (2004). The TALE Homeodomain Protein Pbx2 Is Not Essential for Development and Long-Term Survival. Mol. Cell. Biol. 24: 5324-5331 [Abstract] [Full Text]  
  • Liberzon, A., Ridner, G., Walker, M. D. (2004). Role of intrinsic DNA binding specificity in defining target genes of the mammalian transcription factor PDX1. Nucleic Acids Res 32: 54-64 [Abstract] [Full Text]  
  • Smith, S. B., Gasa, R., Watada, H., Wang, J., Griffen, S. C., German, M. S. (2003). Neurogenin3 and Hepatic Nuclear Factor 1 Cooperate in Activating Pancreatic Expression of Pax4. J. Biol. Chem. 278: 38254-38259 [Abstract] [Full Text]  
  • Giangrande, P. H., Hallstrom, T. C., Tunyaplin, C., Calame, K., Nevins, J. R. (2003). Identification of E-Box Factor TFE3 as a Functional Partner for the E2F3 Transcription Factor. Mol. Cell. Biol. 23: 3707-3720 [Abstract] [Full Text]  
  • Melloul, D., Marshak, S., Cerasi, E. (2002). Regulation of pdx-1 Gene Expression. Diabetes 51: S320-325 [Abstract] [Full Text]  
  • Remacle, S., Shaw-Jackson, C., Matis, C., Lampe, X., Picard, J., Rezsohazy, R. (2002). Changing homeodomain residues 2 and 3 of Hoxa1 alters its activity in a cell-type and enhancer dependent manner. Nucleic Acids Res 30: 2663-2668 [Abstract] [Full Text]  
  • Tremblay, J. J., Viger, R. S. (2001). GATA Factors Differentially Activate Multiple Gonadal Promoters through Conserved GATA Regulatory Elements. Endocrinology 142: 977-986 [Abstract] [Full Text]  
  • Kim, S. K., Hebrok, M. (2001). Intercellular signals regulating pancreas development and function. Genes Dev. 15: 111-127 [Full Text]  
  • Dutta, S., Gannon, M., Peers, B., Wright, C., Bonner-Weir, S., Montminy, M. (2001). PDX:PBX complexes are required for normal proliferation of pancreatic cells during development. Proc. Natl. Acad. Sci. USA 10.1073/pnas.031561298v1 [Abstract] [Full Text]  
  • Peshavaria, M., Cissell, M. A., Henderson, E., Petersen, H. V., Stein, R. (2000). The PDX-1 Activation Domain Provides Specific Functions Necessary for Transcriptional Stimulation in Pancreatic {beta}-Cells. Mol. Endocrinol. 14: 1907-1917 [Abstract] [Full Text]  
  • Dusing, M. R., Brickner, A. G., Lowe, S. Y., Cohen, M. B., Wiginton, D. A. (2000). A duodenum-specific enhancer regulates expression along three axes in the small intestine. Am. J. Physiol. Gastrointest. Liver Physiol. 279: G1080-G1093 [Abstract] [Full Text]  
  • Marshak, S., Benshushan, E., Shoshkes, M., Havin, L., Cerasi, E., Melloul, D. (2000). Functional Conservation of Regulatory Elements in the pdx-1 Gene: PDX-1 and Hepatocyte Nuclear Factor 3beta Transcription Factors Mediate beta -Cell-Specific Expression. Mol. Cell. Biol. 20: 7583-7590 [Abstract] [Full Text]  
  • Denson, L. A., Karpen, S. J., Bogue, C. W., Jacobs, H. C. (2000). Divergent homeobox gene Hex regulates promoter of the Na+-dependent bile acid cotransporter. Am. J. Physiol. Gastrointest. Liver Physiol. 279: G347-G355 [Abstract] [Full Text]  
  • Ohneda, K., Mirmira, R. G., Wang, J., Johnson, J. D., German, M. S. (2000). The Homeodomain of PDX-1 Mediates Multiple Protein-Protein Interactions in the Formation of a Transcriptional Activation Complex on the Insulin Promoter. Mol. Cell. Biol. 20: 900-911 [Abstract] [Full Text]  
  • Glick, E., Leshkowitz, D., Walker, M. D. (2000). Transcription Factor BETA2 Acts Cooperatively with E2A and PDX1 to Activate the Insulin Gene Promoter. J. Biol. Chem. 275: 2199-2204 [Abstract] [Full Text]  
  • Stoffers, D. A., Heller, R. S., Miller, C. P., Habener, J. F. (1999). Developmental Expression of the Homeodomain Protein IDX-1 in Mice Transgenic for an IDX-1 Promoter/lacZ Transcriptional Reporter. Endocrinology 140: 5374-5381 [Abstract] [Full Text]  
  • McWhirter, J. R., Neuteboom, S. T. C., Wancewicz, E. V., Monia, B. P., Downing, J. R., Murre, C. (1999). Oncogenic homeodomain transcription factor E2A-Pbx1 activates a novel WNT gene in pre-B acute lymphoblastoid leukemia. Proc. Natl. Acad. Sci. USA 96: 11464-11469 [Abstract] [Full Text]  
  • Jacobs, Y., Schnabel, C. A., Cleary, M. L. (1999). Trimeric Association of Hox and TALE Homeodomain Proteins Mediates Hoxb2 Hindbrain Enhancer Activity. Mol. Cell. Biol. 19: 5134-5142 [Abstract] [Full Text]  
  • Goudet, G., Delhalle, S., Biemar, F., Martial, J. A., Peers, B. (1999). Functional and Cooperative Interactions between the Homeodomain PDX1, Pbx, and Prep1 Factors on the Somatostatin Promoter. J. Biol. Chem. 274: 4067-4073 [Abstract] [Full Text]  
  • Subramaniam, N., Cairns, W., Okret, S. (1998). Glucocorticoids Repress Transcription from a Negative Glucocorticoid Response Element Recognized by Two Homeodomain-containing Proteins, Pbx and Oct-1. J. Biol. Chem. 273: 23567-23574 [Abstract] [Full Text]  
  • Schwartz, P. T., Vallejo, M. (1998). Differential Regulation of Basal and Cyclic Adenosine 3',5'-Monophosphate-Induced Somatostatin Gene Transcription in Neural Cells by DNA Control Elements That Bind Homeodomain Proteins. Mol. Endocrinol. 12: 1280-1293 [Abstract] [Full Text]  
  • Qiu, Y., Sharma, A., Stein, R. (1998). p300 Mediates Transcriptional Stimulation by the Basic Helix-Loop-Helix Activators of the Insulin Gene. Mol. Cell. Biol. 18: 2957-2964 [Abstract] [Full Text]  
  • Bischof, L. J., Kagawa, N., Moskow, J. J., Takahashi, Y., Iwamatsu, A., Buchberg, A. M., Waterman, M. R. (1998). Members of the Meis1 and Pbx Homeodomain Protein Families Cooperatively Bind a cAMP-responsive Sequence (CRS1) from Bovine CYP17. J. Biol. Chem. 273: 7941-7948 [Abstract] [Full Text]  
  • Milewski, W. M., Duguay, S. J., Chan, S. J., Steiner, D. F. (1998). Conservation of PDX-1 Structure, Function, and Expression in Zebrafish. Endocrinology 139: 1440-1449 [Abstract] [Full Text]  
  • Mailhos, C, Andre, S, Mollereau, B, Goriely, A, Hemmati-Brivanlou, A, Desplan, C (1998). Drosophila Goosecoid requires a conserved heptapeptide for repression of paired-class homeoprotein activators. Development 125: 937-947 [Abstract]  
  • Knoepfler, P. S., Calvo, K. R., Chen, H., Antonarakis, S. E., Kamps, M. P. (1997). Meis1 and pKnox1 bind DNA cooperatively with Pbx1 utilizing an interaction surface disrupted in oncoprotein E2a-Pbx1. Proc. Natl. Acad. Sci. USA 94: 14553-14558 [Abstract] [Full Text]  
  • Gremlich, S., Bonny, C., Waeber, G., Thorens, B. (1997). Fatty Acids Decrease IDX-1 Expression in Rat Pancreatic Islets and Reduce GLUT2, Glucokinase, Insulin, and Somatostatin Levels. J. Biol. Chem. 272: 30261-30269 [Abstract] [Full Text]  
  • Phelan, M. L., Featherstone, M. S. (1997). Distinct HOX N-terminal Arm Residues Are Responsible for Specificity of DNA Recognition by HOX Monomers and HOX·PBX Heterodimers. J. Biol. Chem. 272: 8635-8643 [Abstract] [Full Text]  
  • Peltenburg, L., Murre, C (1997). Specific residues in the Pbx homeodomain differentially modulate the DNA-binding activity of Hox and Engrailed proteins. Development 124: 1089-1098 [Abstract]  
  • Marshak, S., Totary, H., Cerasi, E., Melloul, D. (1996). Purification of the beta -cell glucose-sensitive factor that transactivates the insulin gene differentially in normal and transformed islet cells. Proc. Natl. Acad. Sci. USA 93: 15057-15062 [Abstract] [Full Text]  
  • Schwartz, P. T., Perez-Villamil, B., Rivera, A., Moratalla, R., Vallejo, M. (2000). Pancreatic Homeodomain Transcription Factor IDX1/IPF1 Expressed in Developing Brain Regulates Somatostatin Gene Transcription in Embryonic Neural Cells. J. Biol. Chem. 275: 19106-19114 [Abstract] [Full Text]  
  • Herzig, S., Fuzesi, L., Knepel, W. (2000). Heterodimeric Pbx-Prep1 Homeodomain Protein Binding to the Glucagon Gene Restricting Transcription in a Cell Type-dependent Manner. J. Biol. Chem. 275: 27989-27999 [Abstract] [Full Text]  
  • Smith, S. B., Watada, H., Scheel, D. W., Mrejen, C., German, M. S. (2000). Autoregulation and Maturity Onset Diabetes of the Young Transcription Factors Control the Human PAX4 Promoter. J. Biol. Chem. 275: 36910-36919 [Abstract] [Full Text]  
  • Liu, Y., MacDonald, R. J., Swift, G. H. (2001). DNA Binding and Transcriptional Activation by a PDX1{middle dot}PBX1b{middle dot}MEIS2b Trimer and Cooperation with a Pancreas-specific Basic Helix-Loop-Helix Complex. J. Biol. Chem. 276: 17985-17993 [Abstract] [Full Text]  
  • Dutta, S., Gannon, M., Peers, B., Wright, C., Bonner-Weir, S., Montminy, M. (2001). PDX:PBX complexes are required for normal proliferation of pancreatic cells during development. Proc. Natl. Acad. Sci. USA 98: 1065-1070 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»