This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rosson, D. Articles by O'Brien, T. G. Search for Related Content PubMed PubMed Citation Articles by Rosson, D. Articles by O'Brien, T. G.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 02 1995, 772-779, Vol 15, No. 2
Copyright © 1995, American Society for Microbiology

Constitutive c-myb expression in K562 cells inhibits induced erythroid differentiation but not tetradecanoyl phorbol acetate-induced megakaryocytic differentiation

D Rosson and TG O'Brien
Lankenau Medical Research Center, Wynnewood, Pennsylvania 19096.

K562 cells were stably transfected with a plasmid vector constitutively expressing a full-length human c-myb gene. Parental cells possess the dual potential of inducibility of cellular differentiation along two lineages, i.e., erythroid and megakaryocytic. The resulting lineage is dependent on the inducing agent, with a number of compounds being competent to various degrees for inducing erythroid differentiation, while the tumor promoter tetradecanoyl phorbol acetate (TPA) induces a macrophage-like morphology with enhanced expression of proteins associated with megakaryocytes. Exogeneous expression of c-myb in transfected cell lines abrogated erythroid differentiation induced by cadaverine or cytosine arabinoside as assessed by hemoglobin production. However, TPA-induced megakaryocytic differentiation was left intact, as assessed by cell morphology, cytochemical staining, and the expression of the megakaryocytic antigens. These results indicate that c-Myb and protein kinase C play important roles in cellular differentiation of K562 cells and suggest that agents which directly modulate protein kinase C can induce differentiation in spite of constitutively high levels of c-Myb.

This article has been cited by other articles:

• Zhao, H., Kalota, A., Jin, S., Gewirtz, A. M. (2009). The c-myb proto-oncogene and microRNA-15a comprise an active autoregulatory feedback loop in human hematopoietic cells. Blood 113: 505-516 [Abstract] [Full Text]  
• Martinez, M. C., Larbret, F., Zobairi, F., Coulombe, J., Debili, N., Vainchenker, W., Ruat, M., Freyssinet, J.-M. (2006). Transfer of differentiation signal by membrane microvesicles harboring hedgehog morphogens. Blood 108: 3012-3020 [Abstract] [Full Text]  
• Iwasaki, K., MacKenzie, E. L., Hailemariam, K., Sakamoto, K., Tsuji, Y. (2006). Hemin-Mediated Regulation of an Antioxidant-Responsive Element of the Human Ferritin H Gene and Role of Ref-1 during Erythroid Differentiation of K562 Cells.. Mol. Cell. Biol. 26: 2845-2856 [Abstract] [Full Text]  
• Hegde, S. P., Zhao, J., Ashmun, R. A., Shapiro, L. H. (1999). c-Maf Induces Monocytic Differentiation and Apoptosis in Bipotent Myeloid Progenitors. Blood 94: 1578-1589 [Abstract] [Full Text]  
• Lam, L. T., Ronchini, C., Norton, J., Capobianco, A. J., Bresnick, E. H. (2000). Suppression of Erythroid but Not Megakaryocytic Differentiation of Human K562 Erythroleukemic Cells by Notch-1. J. Biol. Chem. 275: 19676-19684 [Abstract] [Full Text]