This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cockerill, P. N.
Right arrow Articles by Vadas, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cockerill, P. N.
Right arrow Articles by Vadas, M. A.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 04 1995, 2071-2079, Vol 15, No. 4
Copyright © 1995, American Society for Microbiology

Human granulocyte-macrophage colony-stimulating factor enhancer function is associated with cooperative interactions between AP-1 and NFATp/c

PN Cockerill, AG Bert, F Jenkins, GR Ryan, MF Shannon and MA Vadas
Division of Human Immunology, Hanson Centre for Cancer Research, Institute for Medical and Veterinary Science, Adelaide, Australia.

The promoter of the human granulocyte-macrophage colony-stimulating factor gene is regulated by an inducible upstream enhancer. The enhancer encompasses three previously defined binding sites for the transcription factor NFAT (GM170, GM330, and GM550) and a novel NFAT site defined here as the GM420 element. While there was considerable redundancy within the enhancer, the GM330, GM420, and GM550 motifs each functioned efficiently in isolation as enhancer elements and bound NFATp and AP-1 in a highly cooperative fashion. These three NFAT sites closely resembled the distal interleukin-2 NFAT site, and methylation interference assays further defined GGA(N)9TCA as a minimum consensus sequence for this family of NFAT sites. By contrast, the GM170 site, which also had conserved GGA and TCA motifs but in which these motifs were separated by 15 bases, supported strong independent but no cooperative binding of AP-1 and NFATp, and this site functioned poorly as an enhancer element. While both the GM330 and GM420 elements were closely associated with the inducible DNase I-hypersensitive site within the enhancer, the GM420 element was the only NFAT site located within a 160-bp HincII-BalI fragment defined by deletion analysis as the essential core of the enhancer. The GM420 element was unusual, however, in containing a high-affinity NFATp/c-binding sequence (TGGAAAGA) immediately upstream of the sequence TGACATCA which more closely resembled a cyclic AMP response-like element than an AP-1 site.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:

  • Mellstrom, B., Savignac, M., Gomez-Villafuertes, R., Naranjo, J. R. (2008). Ca2+-Operated Transcriptional Networks: Molecular Mechanisms and In Vivo Models. Physiol. Rev. 88: 421-449 [Abstract] [Full Text]  
  • Cockerill, P. N. (2008). NFAT Is Well Placed to Direct Both Enhancer Looping and Domain-Wide Models of Enhancer Function. Sci Signal 1: pe15-pe15 [Abstract] [Full Text]  
  • Bert, A. G., Johnson, B. V., Baxter, E. W., Cockerill, P. N. (2007). A Modular Enhancer Is Differentially Regulated by GATA and NFAT Elements That Direct Different Tissue-Specific Patterns of Nucleosome Positioning and Inducible Chromatin Remodeling. Mol. Cell. Biol. 27: 2870-2885 [Abstract] [Full Text]  
  • Rose, S., Lichtenheld, M., Foote, M. R., Adkins, B. (2007). Murine Neonatal CD4+ Cells Are Poised for Rapid Th2 Effector-Like Function. J. Immunol. 178: 2667-2678 [Abstract] [Full Text]  
  • Kim, H.-P., Korn, L. L., Gamero, A. M., Leonard, W. J. (2005). Calcium-dependent Activation of Interleukin-21 Gene Expression in T Cells. J. Biol. Chem. 280: 25291-25297 [Abstract] [Full Text]  
  • Jee, Y.-K., Gilmour, J., Kelly, A., Bowen, H., Richards, D., Soh, C., Smith, P., Hawrylowicz, C., Cousins, D., Lee, T., Lavender, P. (2005). Repression of Interleukin-5 Transcription by the Glucocorticoid Receptor Targets GATA3 Signaling and Involves Histone Deacetylase Recruitment. J. Biol. Chem. 280: 23243-23250 [Abstract] [Full Text]  
  • Johnson, B. V., Bert, A. G., Ryan, G. R., Condina, A., Cockerill, P. N. (2004). Granulocyte-Macrophage Colony-Stimulating Factor Enhancer Activation Requires Cooperation between NFAT and AP-1 Elements and Is Associated with Extensive Nucleosome Reorganization. Mol. Cell. Biol. 24: 7914-7930 [Abstract] [Full Text]  
  • Bergmann, M. W., Staples, K. J., Smith, S. J., Barnes, P. J., Newton, R. (2004). Glucocorticoid Inhibition of Granulocyte Macrophage-Colony-Stimulating Factor from T cells Is Independent of Control by Nuclear Factor-{kappa}B and Conserved Lymphokine Element 0. Am. J. Respir. Cell Mol. Bio. 30: 555-563 [Abstract] [Full Text]  
  • Lee, D. U., Avni, O., Chen, L., Rao, A. (2004). A Distal Enhancer in the Interferon-{gamma} (IFN-{gamma}) Locus Revealed by Genome Sequence Comparison. J. Biol. Chem. 279: 4802-4810 [Abstract] [Full Text]  
  • Hogan, P. G., Chen, L., Nardone, J., Rao, A. (2003). Transcriptional regulation by calcium, calcineurin, and NFAT. Genes Dev. 17: 2205-2232 [Full Text]  
  • Huang, C., Li, J., Zhang, Q., Huang, X. (2002). Role of Bioavailable Iron in Coal Dust-Induced Activation of Activator Protein-1 and Nuclear Factor of Activated T Cells: Difference between Pennsylvania and Utah Coal Dusts. Am. J. Respir. Cell Mol. Bio. 27: 568-574 [Abstract] [Full Text]  
  • Bower, K. E., Zeller, R. W., Wachsman, W., Martinez, T., McGuire, K. L. (2002). Correlation of Transcriptional Repression by p21SNFT with Changes in DNA{middle dot}NF-AT Complex Interactions. J. Biol. Chem. 277: 34967-34977 [Abstract] [Full Text]  
  • Hawwari, A., Burrows, J., Vadas, M. A., Cockerill, P. N. (2002). The Human IL-3 Locus Is Regulated Cooperatively by Two NFAT-Dependent Enhancers That Have Distinct Tissue-Specific Activities. J. Immunol. 169: 1876-1886 [Abstract] [Full Text]  
  • San-Antonio, B., Iniguez, M. A., Fresno, M. (2002). Protein Kinase Czeta Phosphorylates Nuclear Factor of Activated T Cells and Regulates Its Transactivating Activity. J. Biol. Chem. 277: 27073-27080 [Abstract] [Full Text]  
  • Smith, P. J., Cousins, D. J., Jee, Y.-K., Staynov, D. Z., Lee, T. H., Lavender, P. (2001). Suppression of Granulocyte-Macrophage Colony-Stimulating Factor Expression by Glucocorticoids Involves Inhibition of Enhancer Function by the Glucocorticoid Receptor Binding to Composite NF-AT/Activator Protein-1 Elements. J. Immunol. 167: 2502-2510 [Abstract] [Full Text]  
  • Liu, J., Arai, K.-i., Arai, N. (2001). Inhibition of NFATx Activation by an Oligopeptide: Disrupting the Interaction of NFATx with Calcineurin. J. Immunol. 167: 2677-2687 [Abstract] [Full Text]  
  • Barbeau, B., Robichaud, G. A., Fortin, J.-F., Tremblay, M. J. (2001). Negative Regulation of the NFAT1 Factor by CD45: Implication in HIV-1 Long Terminal Repeat Activation. J. Immunol. 167: 2700-2713 [Abstract] [Full Text]  
  • Cakouros, D., Cockerill, P. N., Bert, A. G., Mital, R., Roberts, D. C., Shannon, M. F. (2001). A NF-{{kappa}}B/Sp1 Region Is Essential for Chromatin Remodeling and Correct Transcription of a Human Granulocyte- Macrophage Colony-Stimulating Factor Transgene. J. Immunol. 167: 302-310 [Abstract] [Full Text]  
  • Bert, A. G., Burrows, J., Hawwari, A., Vadas, M. A., Cockerill, P. N. (2000). Reconstitution of T Cell-Specific Transcription Directed by Composite NFAT/Oct Elements. J. Immunol. 165: 5646-5655 [Abstract] [Full Text]  
  • Iacobelli, M., Wachsman, W., McGuire, K. L. (2000). Repression of IL-2 Promoter Activity by the Novel Basic Leucine Zipper p21SNFT Protein. J. Immunol. 165: 860-868 [Abstract] [Full Text]  
  • Glimcher, L. H., Murphy, K. M. (2000). Lineage commitment in the immune system: the T helper lymphocyte grows up. Genes Dev. 14: 1693-1711 [Full Text]  
  • Bergmann, M., Barnes, P. J., Newton, R. (2000). Molecular Regulation of Granulocyte Macrophage Colony-Stimulating Factor in Human Lung Epithelial Cells by Interleukin (IL)-1beta , IL-4, and IL-13 Involves Both Transcriptional and Post-Transcriptional Mechanisms. Am. J. Respir. Cell Mol. Bio. 22: 582-589 [Abstract] [Full Text]  
  • Himes, S. R., Reeves, R., Attema, J., Nissen, M., Li, Y., Shannon, M. F. (2000). The Role of High-Mobility Group I(Y) Proteins in Expression of IL-2 and T Cell Proliferation. J. Immunol. 164: 3157-3168 [Abstract] [Full Text]  
  • Cockerill, P. N., Bert, A. G., Roberts, D., Vadas, M. A. (1999). The human granulocyte-macrophage colony- stimulating factor gene is autonomously regulated in vivo by an inducible tissue-specific enhancer. Proc. Natl. Acad. Sci. USA 96: 15097-15102 [Abstract] [Full Text]  
  • Shang, C., Attema, J., Cakouros, D., Cockerill, P. N., Shannon, M. F. (1999). Nuclear factor of activated T cells contributes to the function of the CD28 response region of the granulocyte macrophage-colony stimulating factor promoter. Int Immunol 11: 1945-1956 [Abstract] [Full Text]  
  • Sweetser, M. T., Hoey, T., Sun, Y.-L., Weaver, W. M., Price, G. A., Wilson, C. B. (1998). The Roles of Nuclear Factor of Activated T Cells and Ying-Yang 1 in Activation-induced Expression of the Interferon-gamma Promoter in T Cells. J. Biol. Chem. 273: 34775-34783 [Abstract] [Full Text]  
  • Schuh, K., Twardzik, T., Kneitz, B., Heyer, J., Schimpl, A., Serfling, E. (1998). The Interleukin 2 Receptor {alpha} Chain/CD25 Promoter Is a Target for Nuclear Factor of Activated T Cells. JEM 188: 1369-1373 [Abstract] [Full Text]  
  • Towers, T. L., Freedman, L. P. (1998). Granulocyte-Macrophage Colony-stimulating Factor Gene Transcription Is Directly Repressed by the Vitamin D3 Receptor. IMPLICATIONS FOR ALLOSTERIC INFLUENCES ON NUCLEAR RECEPTOR STRUCTURE AND FUNCTION BY A DNA ELEMENT. J. Biol. Chem. 273: 10338-10348 [Abstract] [Full Text]  
  • Bodor, J., Habener, J. F. (1998). Role of Transcriptional Repressor ICER in Cyclic AMP-mediated Attenuation of Cytokine Gene Expression in Human Thymocytes. J. Biol. Chem. 273: 9544-9551 [Abstract] [Full Text]  
  • Amasaki, Y., Masuda, E. S., Imamura, R., Arai, K.-i., Arai, N. (1998). Distinct NFAT Family Proteins Are Involved in the Nuclear NFAT-DNA Binding Complexes from Human Thymocyte Subsets. J. Immunol. 160: 2324-2333 [Abstract] [Full Text]  
  • Beals, C R, Clipstone, N A, Ho, S N, Crabtree, G R (1997). Nuclear localization of NF-ATc by a calcineurin-dependent, cyclosporin-sensitive intramolecular interaction.. Genes Dev. 11: 824-834 [Abstract]  
  • Hodge, M. R., Chun, H. J., Rengarajan, J., Alt, A., Lieberson, R., Glimcher, L. H. (1996). NF-AT-Driven Interleukin-4 Transcription Potentiated by NIP45. Science 274: 1903-1905 [Abstract] [Full Text]  
  • Kochetkova, M., Shannon, M. F. (1996). DNA Triplex Formation Selectively Inhibits Granulocyte-Macrophage Colony-stimulating Factor Gene Expression in Human T Cells. J. Biol. Chem. 271: 14438-14444 [Abstract] [Full Text]  
  • Iniguez, M. A., Martinez-Martinez, S., Punzon, C., Redondo, J. M., Fresno, M. (2000). An Essential Role of the Nuclear Factor of Activated T Cells in the Regulation of the Expression of the Cyclooxygenase-2 Gene in Human T Lymphocytes. J. Biol. Chem. 275: 23627-23635 [Abstract] [Full Text]  
  • Schubert, L. A., Jeffery, E., Zhang, Y., Ramsdell, F., Ziegler, S. F. (2001). Scurfin (FOXP3) Acts as a Repressor of Transcription and Regulates T Cell Activation. J. Biol. Chem. 276: 37672-37679 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»