This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hisamoto, N.
Right arrow Articles by Matsumoto, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hisamoto, N.
Right arrow Articles by Matsumoto, K.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 07 1995, 3767-3776, Vol 15, No. 7
Copyright © 1995, American Society for Microbiology

The EGP1 gene may be a positive regulator of protein phosphatase type 1 in the growth control of Saccharomyces cerevisiae

N Hisamoto, DL Frederick, K Sugimoto, K Tatchell and K Matsumoto
Department of Molecular Biology, Faculty of Science, Nagoya University, Japan.

The Saccharomyces cerevisiae GLC7 gene encodes the catalytic subunit of type 1 protein phosphatase (PP1) and is required for cell growth. A cold-sensitive glc7 mutant (glc7Y170) arrests in G2/M but remains viable at the restrictive temperature. In an effort to identify additional gene products that function in concert with PP1 to regulate growth, we isolated a mutation (gpp1) that exacerbated the growth phenotype of the glc7Y170 mutation, resulting in rapid death of the double mutant at the nonpermissive temperature. We identified an additional gene, EGP1, as an extra-copy suppressor of the glc7Y170 gpp1- 1 double mutant. The nucleotide sequence of EGP1 predicts a leucine- rich repeat protein that is similar to Sds22, a protein from the fission yeast Schizosaccharomyces pombe that positively modulates PP1. EGP1 is essential for cell growth but becomes dispensable upon overexpression of the GLC7 gene. Egp1 and PP1 directly interact, as assayed by coimmunoprecipitation. These results suggest that Egp1 functions as a positive modulator of PP1 in the growth control of S. cerevisiae.


This article has been cited by other articles:

  • Bharucha, J. P., Larson, J. R., Gao, L., Daves, L. K., Tatchell, K. (2008). Ypi1, a Positive Regulator of Nuclear Protein Phosphatase Type 1 Activity in Saccharomyces cerevisiae. Mol. Biol. Cell 19: 1032-1045 [Abstract] [Full Text]  
  • Pedelini, L., Marquina, M., Arino, J., Casamayor, A., Sanz, L., Bollen, M., Sanz, P., Garcia-Gimeno, M. A. (2007). YPI1 and SDS22 Proteins Regulate the Nuclear Localization and Function of Yeast Type 1 Phosphatase Glc7. J. Biol. Chem. 282: 3282-3292 [Abstract] [Full Text]  
  • Santangelo, G. M. (2006). Glucose Signaling in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 70: 253-282 [Abstract] [Full Text]  
  • CEULEMANS, H., BOLLEN, M. (2004). Functional Diversity of Protein Phosphatase-1, a Cellular Economizer and Reset Button. Physiol. Rev. 84: 1-39 [Abstract] [Full Text]  
  • Chang, J. S., Henry, K., Wolf, B. L., Geli, M., Lemmon, S. K. (2002). Protein Phosphatase-1 Binding to Scd5p Is Important for Regulation of Actin Organization and Endocytosis in Yeast. J. Biol. Chem. 277: 48002-48008 [Abstract] [Full Text]  
  • Ceulemans, H., Vulsteke, V., De Maeyer, M., Tatchell, K., Stalmans, W., Bollen, M. (2002). Binding of the Concave Surface of the Sds22 Superhelix to the alpha 4/alpha 5/alpha 6-Triangle of Protein Phosphatase-1. J. Biol. Chem. 277: 47331-47337 [Abstract] [Full Text]  
  • Williams-Hart, T., Wu, X., Tatchell, K. (2002). Protein Phosphatase Type 1 Regulates Ion Homeostasis in Saccharomyces cerevisiae. Genetics 160: 1423-1437 [Abstract] [Full Text]  
  • Peggie, M. W., MacKelvie, S. H., Bloecher, A., Knatko, E. V., Tatchell, K., Stark, M. J. R. (2002). Essential functions of Sds22p in chromosome stability and nuclear localization of PP1. J. Cell Sci. 115: 195-206 [Abstract] [Full Text]  
  • Venturi, G. M., Bloecher, A., Williams-Hart, T., Tatchell, K. (2000). Genetic Interactions Between GLC7, PPZ1 and PPZ2 in Saccharomyces cerevisiae. Genetics 155: 69-83 [Abstract] [Full Text]  
  • Bloecher, A., Tatchell, K. (2000). Dynamic Localization of Protein Phosphatase Type 1 in the Mitotic Cell Cycle of Saccharomyces cerevisiae. JCB 149: 125-140 [Abstract] [Full Text]  
  • Sanz, P., Alms, G. R., Haystead, T. A. J., Carlson, M. (2000). Regulatory Interactions between the Reg1-Glc7 Protein Phosphatase and the Snf1 Protein Kinase. Mol. Cell. Biol. 20: 1321-1328 [Abstract] [Full Text]  
  • Dombek, K. M., Voronkova, V., Raney, A., Young, E. T. (1999). Functional Analysis of the Yeast Glc7-Binding Protein Reg1 Identifies a Protein Phosphatase Type 1-Binding Motif as Essential for Repression of ADH2 Expression. Mol. Cell. Biol. 19: 6029-6040 [Abstract] [Full Text]  
  • Ramaswamy, N. T., Li, L., Khalil, M., Cannon, J. F. (1998). Regulation of Yeast Glycogen Metabolism and Sporulation by Glc7p Protein Phosphatase. Genetics 149: 57-72 [Abstract] [Full Text]  
  • Zheng, J., Khalil, M., Cannon, J. F. (2000). Glc7p Protein Phosphatase Inhibits Expression of Glutamine-Fructose-6-phosphate Transaminase from GFA1. J. Biol. Chem. 275: 18070-18078 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»