This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Del Gatto, F.
Right arrow Articles by Breathnach, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Del Gatto, F.
Right arrow Articles by Breathnach, R.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Sep 1995, 4825-4834, Vol 15, No. 9
Copyright © 1995, American Society for Microbiology

Exon and intron sequences, respectively, repress and activate splicing of a fibroblast growth factor receptor 2 alternative exon

F Del Gatto and R Breathnach
Institut National de la Sante et de la Recherche Medicale U211, Institut de Biologie-Centre Hospitalier Regional, Nantes, France.

Two alternative exons, BEK and K-SAM, code for part of the ligand binding site of fibroblast growth factor receptor 2. Splicing of these exons is mutually exclusive, and the choice between them is made in a tissue-specific manner. We identify here pre-mRNA sequences involved in controlling splicing of the K-SAM exon. The short K-SAM exon sequence 5'-TAGGGCAGGC-3' inhibits splicing of the exon. This inhibition can be overcome by mutating either the exon's 5' or 3' splice site to make it correspond more closely to the relevant consensus sequence. Two separate sequence elements in the intron immediately downstream of the K-SAM exon, one of which is a sequence rich in pyrimidines, are both needed for efficient K-SAM exon splicing. This is no longer the case if either the exon's 5' or 3' splice site is reinforced. Furthermore, if the exon inhibitory sequence is removed, the intron sequences are not required for splicing of the K-SAM exon in a cell line which normally splices this exon. At least three elements are thus involved in controlling splicing of the K-SAM exon: suboptimal 5' and 3' splice sites, an exon inhibitory sequence, and intron activating sequences.


This article has been cited by other articles:

  • Seth, P., Miller, H. B., Lasda, E. L., Pearson, J. L., Garcia-Blanco, M. A. (2008). Identification of an Intronic Splicing Enhancer Essential for the Inclusion of FGFR2 Exon IIIc. J. Biol. Chem. 283: 10058-10067 [Abstract] [Full Text]  
  • Simarro, M., Mauger, D., Rhee, K., Pujana, M. A., Kedersha, N. L., Yamasaki, S., Cusick, M. E., Vidal, M., Garcia-Blanco, M. A., Anderson, P. (2007). Fas-activated serine/threonine phosphoprotein (FAST) is a regulator of alternative splicing. Proc. Natl. Acad. Sci. USA 104: 11370-11375 [Abstract] [Full Text]  
  • Baraniak, A. P., Chen, J. R., Garcia-Blanco, M. A. (2006). Fox-2 Mediates Epithelial Cell-Specific Fibroblast Growth Factor Receptor 2 Exon Choice. Mol. Cell. Biol. 26: 1209-1222 [Abstract] [Full Text]  
  • McAlinden, A., Havlioglu, N., Liang, L., Davies, S. R., Sandell, L. J. (2005). Alternative Splicing of Type II Procollagen Exon 2 Is Regulated by the Combination of a Weak 5' Splice Site and an Adjacent Intronic Stem-loop Cis Element. J. Biol. Chem. 280: 32700-32711 [Abstract] [Full Text]  
  • Sironi, M., Menozzi, G., Comi, G. P., Cagliani, R., Bresolin, N., Pozzoli, U. (2005). Analysis of intronic conserved elements indicates that functional complexity might represent a major source of negative selection on non-coding sequences. Hum Mol Genet 14: 2533-2546 [Abstract] [Full Text]  
  • Lei, H., Vorechovsky, I. (2005). Identification of Splicing Silencers and Enhancers in Sense Alus: a Role for Pseudoacceptors in Splice Site Repression. Mol. Cell. Biol. 25: 6912-6920 [Abstract] [Full Text]  
  • Robson-Dixon, N. D., Garcia-Blanco, M. A. (2004). MAZ Elements Alter Transcription Elongation and Silencing of the Fibroblast Growth Factor Receptor 2 Exon IIIb. J. Biol. Chem. 279: 29075-29084 [Abstract] [Full Text]  
  • SHEN, H., KAN, J. L.C., GHIGNA, C., BIAMONTI, G., GREEN, M. R. (2004). A single polypyrimidine tract binding protein (PTB) binding site mediates splicing inhibition at mouse IgM exons M1 and M2. RNA 10: 787-794 [Abstract] [Full Text]  
  • Sironi, M., Menozzi, G., Riva, L., Cagliani, R., Comi, G. P., Bresolin, N., Giorda, R., Pozzoli, U. (2004). Silencer elements as possible inhibitors of pseudoexon splicing. Nucleic Acids Res 32: 1783-1791 [Abstract] [Full Text]  
  • Baraniak, A. P., Lasda, E. L., Wagner, E. J., Garcia-Blanco, M. A. (2003). A Stem Structure in Fibroblast Growth Factor Receptor 2 Transcripts Mediates Cell-Type-Specific Splicing by Approximating Intronic Control Elements. Mol. Cell. Biol. 23: 9327-9337 [Abstract] [Full Text]  
  • Fomenkov, A., Huang, Y.-P., Topaloglu, O., Brechman, A., Osada, M., Fomenkova, T., Yuriditsky, E., Trink, B., Sidransky, D., Ratovitski, E. (2003). p63{alpha} Mutations Lead to Aberrant Splicing of Keratinocyte Growth Factor Receptor in the Hay-Wells Syndrome. J. Biol. Chem. 278: 23906-23914 [Abstract] [Full Text]  
  • Guil, S., Gattoni, R., Carrascal, M., Abian, J., Stevenin, J., Bach-Elias, M. (2003). Roles of hnRNP A1, SR Proteins, and p68 Helicase in c-H-ras Alternative Splicing Regulation. Mol. Cell. Biol. 23: 2927-2941 [Abstract] [Full Text]  
  • Le Guiner, C., Gesnel, M.-C., Breathnach, R. (2003). TIA-1 or TIAR Is Required for DT40 Cell Viability. J. Biol. Chem. 278: 10465-10476 [Abstract] [Full Text]  
  • MISTRY, N., HARRINGTON, W., LASDA, E., WAGNER, E. J., GARCIA-BLANCO, M. A. (2003). Of urchins and men: Evolution of an alternative splicing unit in fibroblast growth factor receptor genes. RNA 9: 209-217 [Abstract] [Full Text]  
  • Muh, S. J., Hovhannisyan, R. H., Carstens, R. P. (2002). A Non-sequence-specific Double-stranded RNA Structural Element Regulates Splicing of Two Mutually Exclusive Exons of Fibroblast Growth Factor Receptor 2 (FGFR2). J. Biol. Chem. 277: 50143-50154 [Abstract] [Full Text]  
  • Hayakawa, M., Sakashita, E., Ueno, E., Tominaga, S.-i., Hamamoto, T., Kagawa, Y., Endo, H. (2002). Muscle-specific Exonic Splicing Silencer for Exon Exclusion in Human ATP Synthase gamma -Subunit Pre-mRNA. J. Biol. Chem. 277: 6974-6984 [Abstract] [Full Text]  
  • Le Guiner, C., Plet, A., Galiana, D., Gesnel, M.-C., Del Gatto-Konczak, F., Breathnach, R. (2001). Polypyrimidine Tract-binding Protein Represses Splicing of a Fibroblast Growth Factor Receptor-2 Gene Alternative Exon through Exon Sequences. J. Biol. Chem. 276: 43677-43687 [Abstract] [Full Text]  
  • Jones, R. B., Carstens, R. P., Luo, Y., McKeehan, W. L. (2001). 5'- and 3'-terminal nucleotides in the FGFR2 ISAR splicing element core have overlapping roles in exon IIIb activation and exon IIIc repression. Nucleic Acids Res 29: 3557-3565 [Abstract] [Full Text]  
  • Wagner, E. J., Garcia-Blanco, M. A. (2001). Polypyrimidine Tract Binding Protein Antagonizes Exon Definition. Mol. Cell. Biol. 21: 3281-3288 [Full Text]  
  • Zheng, Z.-M., Reid, E. S., Baker, C. C. (2000). Utilization of the Bovine Papillomavirus Type 1 Late-Stage-Specific Nucleotide 3605 3' Splice Site Is Modulated by a Novel Exonic Bipartite Regulator but Not by an Intronic Purine-Rich Element. J. Virol. 74: 10612-10622 [Abstract] [Full Text]  
  • Tomonaga, K., Kobayashi, T., Lee, B. J., Watanabe, M., Kamitani, W., Ikuta, K. (2000). Identification of alternative splicing and negative splicing activity of a nonsegmented negative-strand RNA virus, Borna disease virus. Proc. Natl. Acad. Sci. USA 97: 12788-12793 [Abstract] [Full Text]  
  • Simard, M. J., Chabot, B. (2000). Control of hnRNP A1 Alternative Splicing: an Intron Element Represses Use of the Common 3' Splice Site. Mol. Cell. Biol. 20: 7353-7362 [Abstract] [Full Text]  
  • Del Gatto-Konczak, F., Bourgeois, C. F., Le Guiner, C., Kister, L., Gesnel, M.-C., Stévenin, J., Breathnach, R. (2000). The RNA-Binding Protein TIA-1 Is a Novel Mammalian Splicing Regulator Acting through Intron Sequences Adjacent to a 5' Splice Site. Mol. Cell. Biol. 20: 6287-6299 [Abstract] [Full Text]  
  • Beer, H.-D., Vindevoghel, L., Gait, M. J., Revest, J.-M., Duan, D. R., Mason, I., Dickson, C., Werner, S. (2000). Fibroblast Growth Factor (FGF) Receptor 1-IIIb Is a Naturally Occurring Functional Receptor for FGFs That Is Preferentially Expressed in the Skin and the Brain. J. Biol. Chem. 275: 16091-16097 [Abstract] [Full Text]  
  • Ars, E., Serra, E., de la Luna, S., Estivill, X., Lazaro, C. (2000). Cold shock induces the insertion of a cryptic exon in the neurofibromatosis type 1 (NF1) mRNA. Nucleic Acids Res 28: 1307-1312 [Abstract] [Full Text]  
  • Chew, S. L., Baginsky, L., Eperon, I. C. (2000). An exonic splicing silencer in the testes-specific DNA ligase III {beta} exon. Nucleic Acids Res 28: 402-410 [Abstract] [Full Text]  
  • Chellaiah, A., Yuan, W., Chellaiah, M., Ornitz, D. M. (1999). Mapping Ligand Binding Domains in Chimeric Fibroblast Growth Factor Receptor Molecules. MULTIPLE REGIONS DETERMINE LIGAND BINDING SPECIFICITY. J. Biol. Chem. 274: 34785-34794 [Abstract] [Full Text]  
  • Bourgeois, C. F., Popielarz, M., Hildwein, G., Stevenin, J. (1999). Identification of a Bidirectional Splicing Enhancer: Differential Involvement of SR Proteins in 5' or 3' Splice Site Activation. Mol. Cell. Biol. 19: 7347-7356 [Abstract] [Full Text]  
  • Kuo, B. A., Norton, P. A. (1999). Accurate selection of a 5' splice site requires sequences within fibronectin alternative exon B. Nucleic Acids Res 27: 3945-3952 [Abstract] [Full Text]  
  • Muro, A. F., Caputi, M., Pariyarath, R., Pagani, F., Buratti, E., Baralle, F. E. (1999). Regulation of Fibronectin EDA Exon Alternative Splicing: Possible Role of RNA Secondary Structure for Enhancer Display. Mol. Cell. Biol. 19: 2657-2671 [Abstract] [Full Text]  
  • Puig, O., Gottschalk, A., Fabrizio, P., Séraphin, B. (1999). Interaction of the U1 snRNP with nonconserved intronic sequences affects 5' splice site selection. Genes Dev. 13: 569-580 [Abstract] [Full Text]  
  • Chen, C. D., Kobayashi, R., Helfman, D. M. (1999). Binding of hnRNP H to an exonic splicing silencer is involved in the regulation of alternative splicing of the rat beta -tropomyosin gene. Genes Dev. 13: 593-606 [Abstract] [Full Text]  
  • Zheng, Z. M., He, P.-j., Baker, C. C. (1999). Function of a Bovine Papillomavirus Type 1 Exonic Splicing Suppressor Requires a Suboptimal Upstream 3' Splice Site. J. Virol. 73: 29-36 [Abstract] [Full Text]  
  • Del Gatto-Konczak, F., Olive, M., Gesnel, M.-C., Breathnach, R. (1999). hnRNP A1 Recruited to an Exon In Vivo Can Function as an Exon Splicing Silencer. Mol. Cell. Biol. 19: 251-260 [Abstract] [Full Text]  
  • Hodges, D., Cripps, R. M., O'Connor, M. E., Bernstein, S. I. (1999). The Role of Evolutionarily Conserved Sequences in Alternative Splicing at the 3' End of Drosophila melanogaster Myosin Heavy Chain RNA. Genetics 151: 263-276 [Abstract] [Full Text]  
  • Zheng, Z. M., Huynen, M., Baker, C. C. (1998). A pyrimidine-rich exonic splicing suppressor binds multiple RNA splicing factors and inhibits spliceosome assembly. Proc. Natl. Acad. Sci. USA 95: 14088-14093 [Abstract] [Full Text]  
  • Si, Z.-h., Rauch, D., Stoltzfus, C. M. (1998). The Exon Splicing Silencer in Human Immunodeficiency Virus Type 1 Tat Exon 3 Is Bipartite and Acts Early in Spliceosome Assembly. Mol. Cell. Biol. 18: 5404-5413 [Abstract] [Full Text]  
  • Carstens, R. P., McKeehan, W. L., Garcia-Blanco, M. A. (1998). An Intronic Sequence Element Mediates Both Activation and Repression of Rat Fibroblast Growth Factor Receptor 2 Pre-mRNA Splicing. Mol. Cell. Biol. 18: 2205-2217 [Abstract] [Full Text]  
  • Min, H, Turck, C W, Nikolic, J M, Black, D L (1997). A new regulatory protein, KSRP, mediates exon inclusion through an intronic splicing enhancer.. Genes Dev. 11: 1023-1036 [Abstract]  
  • Cote, G. J., Huang, E. S-C., Jin, W., Morrison, R. S. (1997). Sequence Requirements for Regulated RNA Splicing of the Human Fibroblast Growth Factor Receptor-1 alpha Exon. J. Biol. Chem. 272: 1054-1060 [Abstract] [Full Text]  
  • Pret, A.-M., Fiszman, M. Y. (1996). Sequence Divergence Associated with Species-specific Splicing of the Nonmuscle beta-Tropomyosin Alternative Exon. J. Biol. Chem. 271: 11511-11517 [Abstract] [Full Text]  
  • Jones, R. B., Wang, F., Luo, Y., Yu, C., Jin, C., Suzuki, T., Kan, M., McKeehan, W. L. (2001). The Nonsense-mediated Decay Pathway and Mutually Exclusive Expression of Alternatively Spliced FGFR2IIIb and -IIIc mRNAs. J. Biol. Chem. 276: 4158-4167 [Abstract] [Full Text]  
  • Genetta, T., Morisaki, H., Morisaki, T., Holmes, E. W. (2001). A Novel Bipartite Intronic Splicing Enhancer Promotes the Inclusion of a Mini-exon in the AMP Deaminase 1 Gene. J. Biol. Chem. 276: 25589-25597 [Abstract] [Full Text]  
  • Le Guiner, C., Lejeune, F., Galiana, D., Kister, L., Breathnach, R., Stevenin, J., Del Gatto-Konczak, F. (2001). TIA-1 and TIAR Activate Splicing of Alternative Exons with Weak 5' Splice Sites followed by a U-rich Stretch on Their Own Pre-mRNAs. J. Biol. Chem. 276: 40638-40646 [Abstract] [Full Text]  
  • Burgar, H. R., Burns, H. D., Elsden, J. L., Lalioti, M. D., Heath, J. K. (2002). Association of the Signaling Adaptor FRS2 with Fibroblast Growth Factor Receptor 1 (Fgfr1) Is Mediated by Alternative Splicing of the Juxtamembrane Domain. J. Biol. Chem. 277: 4018-4023 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»