This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Preston, G. A.
Right arrow Articles by Barrett, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Preston, G. A.
Right arrow Articles by Barrett, J. C.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Jan 1996, 211-218, Vol 16, No. 1
Copyright © 1996, American Society for Microbiology

Induction of apoptosis by c-Fos protein

GA Preston, TT Lyon, Y Yin, JE Lang, G Solomon, L Annab, DG Srinivasan, DA Alcorta and JC Barrett
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA.

The role of c-Fos in apoptosis was examined in two Syrian hamster embryo cell lines (sup+I and sup-II) and a human colorectal carcinoma cell line (RKO), using the chimeric Fos-estrogen receptor fusion protein c-FosER. As previously reported, contrasting responses were observed when these two cell lines were placed under growth factor deprivation conditions; sup+I cells were highly susceptible to apoptosis, whereas sup-II cells were resistant. In this report, we show that the activated c-FosER protein induces apoptosis in sup-II preneoplastic cells in serum-free medium, indicating that c-Fos protein can induce apoptotic cell death in these cells. c-Fos-induced apoptosis was not blocked by the protein synthesis inhibitor cycloheximide, suggesting that the c-Fos transcriptional activation activity is not involved. This conclusion was further supported by the observation that overexpression of v-Fos, which is highly proficient in transcriptional activation but deficient in the transcriptional repression activity associated with c-Fos, did not induce apoptosis. Constitutively expressed Bcl-2 delayed the onset of low-serum-induced apoptosis in sup+I cells and enhanced survival in sup-II cells. Further, coexpression of Bcl-2 and c-FosER in sup+I or sup-II cells protected the cells from c-FosER-induced apoptosis. The possibility that c-FosER- induced apoptosis requires a p53 function was examined. Colorectal carcinoma RKOp53+/+ cells, which do not normally undergo apoptosis in serum-free medium, showed apoptotic DNA fragmentation upon expression and activation of c-FosER.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:

  • Kim, E.-H., Na, H.-K., Kim, D.-H., Park, S.-A., Kim, H.-N., Song, N.-Y., Surh, Y.-J. (2008). 15-Deoxy-{Delta}12,14-prostaglandin J2 induces COX-2 expression through Akt-driven AP-1 activation in human breast cancer cells: a potential role of ROS. Carcinogenesis 29: 688-695 [Abstract] [Full Text]  
  • Sharma, S. V., Settleman, J. (2007). Oncogene addiction: setting the stage for molecularly targeted cancer therapy. Genes Dev. 21: 3214-3231 [Abstract] [Full Text]  
  • Zhang, X., Zhang, L., Yang, H., Huang, X., Otu, H., Libermann, T. A., DeWolf, W. C., Khosravi-Far, R., Olumi, A. F. (2007). c-Fos as a Proapoptotic Agent in TRAIL-Induced Apoptosis in Prostate Cancer Cells. Cancer Res. 67: 9425-9434 [Abstract] [Full Text]  
  • Jin, Y. J., Wang, J., Qiao, C., Hei, T. K., Brandt-Rauf, P. W., Yin, Y. (2006). A Novel Mechanism for p53 to Regulate Its Target Gene ECK in Signaling Apoptosis. Mol Cancer Res 4: 769-778 [Abstract] [Full Text]  
  • Preston, G. A., Zarella, C. S., Pendergraft, W. F. III, Rudolph, E. H., Yang, J. J., Sekura, S. B., Jennette, J. C., Falk, R. J. (2002). Novel Effects of Neutrophil-Derived Proteinase 3 and Elastase on the Vascular Endothelium Involve In Vivo Cleavage of NF-{kappa}B and Proapoptotic Changes in JNK, ERK, and p38 MAPK Signaling Pathways. J. Am. Soc. Nephrol. 13: 2840-2849 [Abstract] [Full Text]  
  • Schmitt, J.P., Schunkert, H., Birnbaum, D.E., Aebert, H. (2002). Kinetics of heat shock protein 70 synthesis in the human heart after cold cardioplegic arrest. Eur. J. Cardiothorac. Surg. 22: 415-420 [Abstract] [Full Text]  
  • Stein, T. D., Johnson, J. A. (2002). Lack of Neurodegeneration in Transgenic Mice Overexpressing Mutant Amyloid Precursor Protein Is Associated with Increased Levels of Transthyretin and the Activation of Cell Survival Pathways. J. Neurosci. 22: 7380-7388 [Abstract] [Full Text]  
  • Palmada, M., Kanwal, S., Rutkoski, N.J., Gustafson-Brown, C., Johnson, R.S., Wisdom, R., Carter, B.D. (2002). c-jun is essential for sympathetic neuronal death induced by NGF withdrawal but not by p75 activation. JCB 158: 453-461 [Abstract] [Full Text]  
  • Amendt, C., Mann, A., Schirmacher, P., Blessing, M. (2002). Resistance of keratinocytes to TGF{beta}-mediated growth restriction and apoptosis induction accelerates re-epithelialization in skin wounds. J. Cell Sci. 115: 2189-2198 [Abstract] [Full Text]  
  • Schmitt, J. P., Schroder, J., Schunkert, H., Birnbaum, D. E., Aebert, H. (2002). Role of apoptosis in myocardial stunning after open heart surgery. Ann. Thorac. Surg. 73: 1229-1235 [Abstract] [Full Text]  
  • Matsuda, A., Suzuki, Y., Kondo, K., Ikeda, Y., Umemura, K. (2002). Hypercholesterolemia induces regression in neointimal thickening due to apoptosis of vascular smooth muscle cells in the hamster endothelial injury model. Cardiovasc Res 53: 512-523 [Abstract] [Full Text]  
  • Eichhorst, S. T., Müller, M., Li-Weber, M., Schulze-Bergkamen, H., Angel, P., Krammer, P. H. (2000). A Novel AP-1 Element in the CD95 Ligand Promoter Is Required for Induction of Apoptosis in Hepatocellular Carcinoma Cells upon Treatment with Anticancer Drugs. Mol. Cell. Biol. 20: 7826-7837 [Abstract] [Full Text]  
  • Matsuoka, M., Wispriyono, B., Iryo, Y., Igisu, H. (2000). Mercury Chloride Activates c-Jun N-Terminal Kinase and Induces c-jun Expression in LLC-PK1 Cells. Toxicol Sci 53: 361-368 [Abstract] [Full Text]  
  • Ubeda, M., Vallejo, M., Habener, J. F. (1999). CHOP Enhancement of Gene Transcription by Interactions with Jun/Fos AP-1 Complex Proteins. Mol. Cell. Biol. 19: 7589-7599 [Abstract] [Full Text]  
  • Galea, J, Armstrong, J, Francis, S.E, Cooper, G, Crossman, D.C, Holt, C.M (1999). Alterations in c-fos expression, cell proliferation and apoptosis in pressure distended human saphenous vein. Cardiovasc Res 44: 436-448 [Abstract] [Full Text]  
  • Zanella, C. L., Timblin, C. R., Cummins, A., Jung, M., Goldberg, J., Raabe, R., Tritton, T. R., Mossman, B. T. (1999). Asbestos-induced phosphorylation of epidermal growth factor receptor is linked to c-fos and apoptosis. Am. J. Physiol. Lung Cell. Mol. Physiol. 277: L684-L693 [Abstract] [Full Text]  
  • Horowitz, S. (1999). Pathways to Cell Death in Hyperoxia. Chest 116 : 64S-67S [Full Text]  
  • Elkeles, A., Juven-Gershon, T., Israeli, D., Wilder, S., Zalcenstein, A., Oren, M. (1999). The c-fos Proto-Oncogene Is a Target for Transactivation by the p53 Tumor Suppressor. Mol. Cell. Biol. 19: 2594-2600 [Abstract] [Full Text]  
  • Sun, S.-Y., Yue, P., Lotan, R. (1999). Induction of Apoptosis by N-(4-Hydroxyphenyl)retinamide and Its Association with Reactive Oxygen Species, Nuclear Retinoic Acid Receptors, and Apoptosis-Related Genes in Human Prostate Carcinoma Cells. Mol. Pharmacol. 55: 403-410 [Abstract] [Full Text]  
  • Townsend, K. J., Zhou, P., Qian, L., Bieszczad, C. K., Lowrey, C. H., Yen, A., Craig, R. W. (1999). Regulation of MCL1 through a Serum Response Factor/Elk-1-mediated Mechanism Links Expression of a Viability-promoting Member of the BCL2 Family to the Induction of Hematopoietic Cell Differentiation. J. Biol. Chem. 274: 1801-1813 [Abstract] [Full Text]  
  • Sharma, S. K., Chapman, D., Temsah, R., Netticadan, T., Brasil, D. P., Dhalla, N. S. (1999). Prevention of Vascular Apoptosis in Myocardial Infarction by Losartan. J CARDIOVASC PHARMACOL THER 4: 77-84 [Abstract]  
  • Kustikova, O., Kramerov, D., Grigorian, M., Berezin, V., Bock, E., Lukanidin, E., Tulchinsky, E. (1998). Fra-1 Induces Morphological Transformation and Increases In Vitro Invasiveness and Motility of Epithelioid Adenocarcinoma Cells. Mol. Cell. Biol. 18: 7095-7105 [Abstract] [Full Text]  
  • Wispriyono, B., Matsuoka, M., Igisu, H., Matsuno, K. (1998). Protection from Cadmium Cytotoxicity by N-Acetylcysteine in LLC-PK1 Cells. J. Pharmacol. Exp. Ther. 287: 344-351 [Abstract] [Full Text]  
  • He, H., Qi, X.-M., Grossmann, J., Distelhorst, C. W. (1998). c-Fos Degradation by the Proteasome. AN EARLY, Bcl-2-REGULATED STEP IN APOPTOSIS. J. Biol. Chem. 273: 25015-25019 [Abstract] [Full Text]  
  • Slotkin, T. A. (1998). Fetal Nicotine or Cocaine Exposure: Which One is Worse?. J. Pharmacol. Exp. Ther. 285: 931-945 [Abstract] [Full Text]  
  • Mao, D., Warner, E. A., Gurwitch, S. A., Dowd, D. R. (1998). Differential Regulation and Transcriptional Control of Immediate Early Gene Expression in Forskolin-Treated WEHI7.2 Thymoma Cells. Mol. Endocrinol. 12: 492-503 [Abstract] [Full Text]  
  • Adachi, H., Preston, G., Harvat, B., Dawson, M. I., Jetten, A. M. (1998). Inhibition of Cell Proliferation and Induction of Apoptosis by the Retinoid AHPN in Human Lung Carcinoma Cells. Am. J. Respir. Cell Mol. Bio. 18: 323-333 [Abstract] [Full Text]  
  • Janssen, Y. M. W., Matalon, S., Mossman, B. T. (1997). Differential induction of c-fos, c-jun, and apoptosis in lung epithelial cells exposed to ROS or RNS. Am. J. Physiol. Lung Cell. Mol. Physiol. 273: L789-L796 [Abstract] [Full Text]  
  • Nair, P., Muthukkumar, S., Sells, S. F., Han, S.-S., Sukhatme, V. P., Rangnekar, V. M. (1997). Early Growth Response-1-dependent Apoptosis Is Mediated by p53. J. Biol. Chem. 272: 20131-20138 [Abstract] [Full Text]  
  • Ivanov, V. N., Ivanov, V. N. (1997). Transcription Factor Activation during Signal-induced Apoptosis of Immature CD4+CD8+ Thymocytes. A PROTECTIVE ROLE OF c-Fos. J. Biol. Chem. 272: 8558-8566 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»