This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shi, X.
Right arrow Articles by Jaehning, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shi, X.
Right arrow Articles by Jaehning, J. A.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Feb 1996, 669-676, Vol 16, No. 2
Copyright © 1996, American Society for Microbiology

Paf1p, an RNA polymerase II-associated factor in Saccharomyces cerevisiae, may have both positive and negative roles in transcription

X Shi, A Finkelstein, AJ Wolf, PA Wade, ZF Burton and JA Jaehning
Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver 80262, USA.

Regulated transcription initiation requires, in addition to RNA polymerase II and the general transcription factors, accessory factors termed mediators or adapters. We have used affinity chromatography to identify a collection of factors that associate with Saccharomyces cerevisiae RNA polymerase II (P. A. Wade, W. Werel, R. C. Fentzke, N. E. Thompson, J. F. Leykam, R. R. Burgess, J. A. Jaehning, and Z. F. Burton, submitted for publication). Here we report identification and characterization of a gene encoding one of these factors, PAF1 (for RNA polymerase-associated factor 1). PAF1 encodes a novel, highly charged protein of 445 amino acids. Disruption of PAF1 in S. cerevisiae leads to pleiotropic phenotypic traits, including slow growth, temperature sensitivity, and abnormal cell morphology. Consistent with a possible role in transcription, Paf1p is localized to the nucleus. By comparing the abundances of many yeast transcripts in isogenic wild-type and paf1 mutant strains, we have identified genes whose expression is affected by PAF1. In particular, disruption of PAF1 decreases the induction of the galactose-regulated genes three- to fivefold. In contrast, the transcript level of MAK16, an essential gene involved in cell cycle regulation, is greatly increased in the paf1 mutant strain. Paf1p may therefore be required for both positive and negative regulation of subsets of yeast genes. Like Paf1p, the GAL11 gene product is found associated with RNA polymerase II and is required for regulated expression of many yeast genes including those controlled by galactose. We have found that a gal11 paf1 double mutant has a much more severe growth defect than either of the single mutants, indicating that these two proteins may function in parallel pathways to communicate signals from regulatory factors to RNA polymerase II.


This article has been cited by other articles:

  • Kim, J., Roeder, R. G. (2009). Direct Bre1-Paf1 Complex Interactions and RING Finger-independent Bre1-Rad6 Interactions Mediate Histone H2B Ubiquitylation in Yeast. J. Biol. Chem. 284: 20582-20592 [Abstract] [Full Text]  
  • Rodriguez-Paredes, M., Ceballos-Chavez, M., Esteller, M., Garcia-Dominguez, M., Reyes, J. C. (2009). The chromatin remodeling factor CHD8 interacts with elongating RNA polymerase II and controls expression of the cyclin E2 gene. Nucleic Acids Res 37: 2449-2460 [Abstract] [Full Text]  
  • Strawn, L. A., Lin, C. A., Tank, E. M.H., Osman, M. M., Simpson, S. A., True, H. L. (2009). Mutants of the Paf1 Complex Alter Phenotypic Expression of the Yeast Prion [PSI+]. Mol. Biol. Cell 20: 2229-2241 [Abstract] [Full Text]  
  • Youn, M.-Y., Yoo, H.-S., Kim, M.-J., Hwang, S.-Y., Choi, Y., Desiderio, S. V., Yoo, J.-Y. (2007). hCTR9, a Component of Paf1 Complex, Participates in the Transcription of Interleukin 6-responsive Genes through Regulation of STAT3-DNA Interactions. J. Biol. Chem. 282: 34727-34734 [Abstract] [Full Text]  
  • Osley, M. A. (2006). Regulation of histone H2A and H2B ubiquitylation. Brief Funct Genomic Proteomic 5: 179-189 [Abstract] [Full Text]  
  • Reinberg, D., Sims, R. J. III (2006). de FACTo Nucleosome Dynamics. J. Biol. Chem. 281: 23297-23301 [Abstract] [Full Text]  
  • Game, J. C., Williamson, M. S., Spicakova, T., Brown, J. M. (2006). The RAD6/BRE1 Histone Modification Pathway in Saccharomyces Confers Radiation Resistance Through a RAD51-Dependent Process That Is Independent of RAD18. Genetics 173: 1951-1968 [Abstract] [Full Text]  
  • Qiu, H., Hu, C., Wong, C.-M., Hinnebusch, A. G. (2006). The Spt4p Subunit of Yeast DSIF Stimulates Association of the Paf1 Complex with Elongating RNA Polymerase II. Mol. Cell. Biol. 26: 3135-3148 [Abstract] [Full Text]  
  • Adelman, K., Wei, W., Ardehali, M. B., Werner, J., Zhu, B., Reinberg, D., Lis, J. T. (2006). Drosophila Paf1 Modulates Chromatin Structure at Actively Transcribed Genes. Mol. Cell. Biol. 26: 250-260 [Abstract] [Full Text]  
  • Prather, D., Krogan, N. J., Emili, A., Greenblatt, J. F., Winston, F. (2005). Identification and Characterization of Elf1, a Conserved Transcription Elongation Factor in Saccharomyces cerevisiae. Mol. Cell. Biol. 25: 10122-10135 [Abstract] [Full Text]  
  • Zhu, B., Mandal, S. S., Pham, A.-D., Zheng, Y., Erdjument-Bromage, H., Batra, S. K., Tempst, P., Reinberg, D. (2005). The human PAF complex coordinates transcription with events downstream of RNA synthesis. Genes Dev. 19: 1668-1673 [Abstract] [Full Text]  
  • Rozenblatt-Rosen, O., Hughes, C. M., Nannepaga, S. J., Shanmugam, K. S., Copeland, T. D., Guszczynski, T., Resau, J. H., Meyerson, M. (2005). The Parafibromin Tumor Suppressor Protein Is Part of a Human Paf1 Complex. Mol. Cell. Biol. 25: 612-620 [Abstract] [Full Text]  
  • Porter, S. E., Penheiter, K. L., Jaehning, J. A. (2005). Separation of the Saccharomyces cerevisiae Paf1 Complex from RNA Polymerase II Results in Changes in Its Subnuclear Localization. Eukaryot Cell 4: 209-220 [Abstract] [Full Text]  
  • He, Y., Doyle, M. R., Amasino, R. M. (2004). PAF1-complex-mediated histone methylation of FLOWERING LOCUS C chromatin is required for the vernalization-responsive, winter-annual habit in Arabidopsis. Genes Dev. 18: 2774-2784 [Abstract] [Full Text]  
  • Sims, R. J. III, Belotserkovskaya, R., Reinberg, D. (2004). Elongation by RNA polymerase II: the short and long of it. Genes Dev. 18: 2437-2468 [Abstract] [Full Text]  
  • Carvin, C. D., Kladde, M. P. (2004). Effectors of Lysine 4 Methylation of Histone H3 in Saccharomyces cerevisiae Are Negative Regulators of PHO5 and GAL1-10. J. Biol. Chem. 279: 33057-33062 [Abstract] [Full Text]  
  • Rani, P. G., Ranish, J. A., Hahn, S. (2004). RNA Polymerase II (Pol II)-TFIIF and Pol II-Mediator Complexes: the Major Stable Pol II Complexes and Their Activity in Transcription Initiation and Reinitiation. Mol. Cell. Biol. 24: 1709-1720 [Abstract] [Full Text]  
  • Mason, P. B., Struhl, K. (2003). The FACT Complex Travels with Elongating RNA Polymerase II and Is Important for the Fidelity of Transcriptional Initiation In Vivo. Mol. Cell. Biol. 23: 8323-8333 [Abstract] [Full Text]  
  • Henry, K. W., Wyce, A., Lo, W.-S., Duggan, L. J., Emre, N.C. T., Kao, C.-F., Pillus, L., Shilatifard, A., Osley, M. A., Berger, S. L. (2003). Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation, mediated by SAGA-associated Ubp8. Genes Dev. 17: 2648-2663 [Abstract] [Full Text]  
  • Porter, S. E., Washburn, T. M., Chang, M., Jaehning, J. A. (2002). The Yeast Paf1-RNA Polymerase II Complex Is Required for Full Expression of a Subset of Cell Cycle-Regulated Genes. Eukaryot Cell 1: 830-842 [Abstract] [Full Text]  
  • Mueller, C. L., Jaehning, J. A. (2002). Ctr9, Rtf1, and Leo1 Are Components of the Paf1/RNA Polymerase II Complex. Mol. Cell. Biol. 22: 1971-1980 [Abstract] [Full Text]  
  • Nelson, L. D., Musso, M., Van Dyke, M. W. (2000). The Yeast STM1 Gene Encodes a Purine Motif Triple Helical DNA-binding Protein. J. Biol. Chem. 275: 5573-5581 [Abstract] [Full Text]  
  • Davies, J. P., Yildiz, F. H., Grossman, A. R. (1999). Sac3, an Snf1-like Serine/Threonine Kinase That Positively and Negatively Regulates the Responses of Chlamydomonas to Sulfur Limitation. Plant Cell 11: 1179-1190 [Abstract] [Full Text]  
  • Chang, M., French-Cornay, D., Fan, H.-y., Klein, H., Denis, C. L., Jaehning, J. A. (1999). A Complex Containing RNA Polymerase II, Paf1p, Cdc73p, Hpr1p, and Ccr4p Plays a Role in Protein Kinase C Signaling. Mol. Cell. Biol. 19: 1056-1067 [Abstract] [Full Text]  
  • Gancedo, J. M. (1998). Yeast Carbon Catabolite Repression. Microbiol. Mol. Biol. Rev. 62: 334-361 [Abstract] [Full Text]  
  • Hampsey, M. (1998). Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery. Microbiol. Mol. Biol. Rev. 62: 465-503 [Abstract] [Full Text]  
  • Trifa, Y., Privat, I., Gagnon, J., Baeza, L., Lerbs-Mache, S. (1998). The Nuclear RPL4 Gene Encodes a Chloroplast Protein That Co-purifies with the T7-like Transcription Complex as Well as Plastid Ribosomes. J. Biol. Chem. 273: 3980-3985 [Abstract] [Full Text]  
  • Saleh, A., Lang, V., Cook, R., Brandl, C. J. (1997). Identification of Native Complexes Containing the Yeast Coactivator/Repressor Proteins NGG1/ADA3 and ADA2. J. Biol. Chem. 272: 5571-5578 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»