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Mol. Cell. Biol., 05 1996, 2226-2237, Vol 16, No. 5
Copyright © 1996, American Society for Microbiology

Calcineurin inhibits VCX1-dependent H+/Ca2+ exchange and induces Ca2+ ATPases in Saccharomyces cerevisiae

KW Cunningham and GR Fink
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA.

The PMC1 gene in Saccharomyces cerevisiae encodes a vacuolar Ca2+ ATPase required for growth in high-Ca2+ conditions. Previous work showed that Ca2+ tolerance can be restored to pmc1 mutants by inactivation of calcineurin, a Ca2+/calmodulin-dependent protein phosphatase sensitive to the immunosuppressive drug FK506. We now report that calcineurin decreases Ca2+ tolerance of pmc1 mutants by inhibiting the function of VCX1, which encodes a vacuolar H+/Ca2+ exchanger related to vertebrate Na+/Ca2+ exchangers. The contribution of VCX1 in Ca2+ tolerance is low in strains with a functional calcineurin and is high in strains which lack calcineurin activity. In contrast, the contribution of PMC1 to Ca2+ tolerance is augmented by calcineurin activation. Consistent with these positive and negative roles of calcineurin, expression of a vcx1::lacZ reporter was slightly diminished and a pmc1::lacZ reporter was induced up to 500-fold by processes dependent on calcineurin, calmodulin, and Ca2+. It is likely that calcineurin inhibits VCX1 function mainly by posttranslational mechanisms. Activities of VCX1 and PMC1 help to control cytosolic free Ca2+ concentrations because their function can decrease pmc1::lacZ induction by calcineurin. Additional studies with reporter genes and mutants indicate that PMR1 and PMR2A, encoding P-type ion pumps required for Mn2+ and Na+ tolerance, may also be induced physiologically in response to high-Mn2+ and -Na+ conditions through calcineurin-dependent mechanisms. In these situations, inhibition of VCX1 function may be important for the production of Ca2+ signals. We propose that elevated cytosolic free Ca2+ concentrations, calmodulin, and calcineurin regulate at least four ion transporters in S. cerevisiae in response to several environmental conditions.


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  • Aiello, D. P., Fu, L., Miseta, A., Bedwell, D. M. (2002). Intracellular Glucose 1-Phosphate and Glucose 6-Phosphate Levels Modulate Ca2+ Homeostasis in Saccharomyces cerevisiae. J. Biol. Chem. 277: 45751-45758 [Abstract] [Full Text]  
  • Pittman, J. K., Sreevidya, C. S., Shigaki, T., Ueoka-Nakanishi, H., Hirschi, K. D. (2002). Distinct N-Terminal Regulatory Domains of Ca2+/H+ Antiporters. Plant Physiol. 130: 1054-1062 [Abstract] [Full Text]  
  • Yoshimoto, H., Saltsman, K., Gasch, A. P., Li, H. X., Ogawa, N., Botstein, D., Brown, P. O., Cyert, M. S. (2002). Genome-wide Analysis of Gene Expression Regulated by the Calcineurin/Crz1p Signaling Pathway in Saccharomyces cerevisiae. J. Biol. Chem. 277: 31079-31088 [Abstract] [Full Text]  
  • Cheng, H., Sugiura, R., Wu, W., Fujita, M., Lu, Y., Sio, S. O., Kawai, R., Takegawa, K., Shuntoh, H., Kuno, T. (2002). Role of the Rab GTP-Binding Protein Ypt3 in the Fission Yeast Exocytic Pathway and Its Connection to Calcineurin Function. Mol. Biol. Cell 13: 2963-2976 [Abstract] [Full Text]  
  • Pittman, J. K., Shigaki, T., Cheng, N.-H., Hirschi, K. D. (2002). Mechanism of N-terminal Autoinhibition in the Arabidopsis Ca2+/H+ Antiporter CAX1. J. Biol. Chem. 277: 26452-26459 [Abstract] [Full Text]  
  • Cronin, S. R., Rao, R., Hampton, R. Y. (2002). Cod1p/Spf1p is a P-type ATPase involved in ER function and Ca2+ homeostasis. JCB 157: 1017-1028 [Abstract] [Full Text]  
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  • Hohmann, S. (2002). Osmotic Stress Signaling and Osmoadaptation in Yeasts. Microbiol. Mol. Biol. Rev. 66: 300-372 [Abstract] [Full Text]  
  • Williams-Hart, T., Wu, X., Tatchell, K. (2002). Protein Phosphatase Type 1 Regulates Ion Homeostasis in Saccharomyces cerevisiae. Genetics 160: 1423-1437 [Abstract] [Full Text]  
  • Boustany, L. M., Cyert, M. S. (2002). Calcineurin-dependent regulation of Crz1p nuclear export requires Msn5p and a conserved calcineurin docking site. Genes Dev. 16: 608-619 [Abstract] [Full Text]  
  • Ton, V.-K., Mandal, D., Vahadji, C., Rao, R. (2002). Functional Expression in Yeast of the Human Secretory Pathway Ca2+, Mn2+-ATPase Defective in Hailey-Hailey Disease. J. Biol. Chem. 277: 6422-6427 [Abstract] [Full Text]  
  • Muller, E. M., Locke, E. G., Cunningham, K. W. (2001). Differential Regulation of Two Ca2+ Influx Systems by Pheromone Signaling in Saccharomyces cerevisiae. Genetics 159: 1527-1538 [Abstract] [Full Text]  
  • Shigaki, T., Cheng, N.-h., Pittman, J. K., Hirschi, K. (2001). Structural Determinants of Ca2+ Transport in the Arabidopsis H+/Ca2+ Antiporter CAX1. J. Biol. Chem. 276: 43152-43159 [Abstract] [Full Text]  
  • Pittman, J. K., Hirschi, K. D. (2001). Regulation of CAX1, an Arabidopsis Ca2+/H+ Antiporter. Identification of an N-Terminal Autoinhibitory Domain. Plant Physiol. 127: 1020-1029 [Abstract] [Full Text]  
  • Polizotto, R. S., Cyert, M. S. (2001). Calcineurin-dependent nuclear import of the transcription factor Crz1p requires Nmd5p. JCB 154: 951-960 [Abstract] [Full Text]  
  • Maser, P., Thomine, S., Schroeder, J. I., Ward, J. M., Hirschi, K., Sze, H., Talke, I. N., Amtmann, A., Maathuis, F. J.M., Sanders, D., Harper, J. F., Tchieu, J., Gribskov, M., Persans, M. W., Salt, D. E., Kim, S. A, Guerinot, M. L. (2001). Phylogenetic Relationships within Cation Transporter Families of Arabidopsis. Plant Physiol. 126: 1646-1667 [Abstract] [Full Text]  
  • Simão, R. d. C. G., Gomes, S. L. (2001). Structure, Expression, and Functional Analysis of the Gene Coding for Calmodulin in the Chytridiomycete Blastocladiella emersonii. J. Bacteriol. 183: 2280-2288 [Abstract] [Full Text]  
  • Geisler, M., Frangne, N., Gomès, E., Martinoia, E., Palmgren, M. G. (2000). The ACA4 Gene of Arabidopsis Encodes a Vacuolar Membrane Calcium Pump That Improves Salt Tolerance in Yeast. Plant Physiol. 124: 1814-1827 [Abstract] [Full Text]  
  • Rusnak, F., Mertz, P. (2000). Calcineurin: Form and Function. Physiol. Rev. 80: 1483-1521 [Abstract] [Full Text]  
  • Locke, E. G., Bonilla, M., Liang, L., Takita, Y., Cunningham, K. W. (2000). A Homolog of Voltage-Gated Ca2+ Channels Stimulated by Depletion of Secretory Ca2+ in Yeast. Mol. Cell. Biol. 20: 6686-6694 [Abstract] [Full Text]  
  • Ueoka-Nakanishi, H., Maeshima, M. (2000). Quantification of Ca2+/H+ Antiporter VCAX1p in Vacuolar Membranes and Its Absence in Roots of Mung Bean. Plant Cell Physiol 41: 1067-1071 [Abstract] [Full Text]  
  • Hirschi, K. D., Korenkov, V. D., Wilganowski, N. L., Wagner, G. J. (2000). Expression of Arabidopsis CAX2 in Tobacco. Altered Metal Accumulation and Increased Manganese Tolerance. Plant Physiol. 124: 125-134 [Abstract] [Full Text]  
  • Kingsbury, T. J., Cunningham, K. W. (2000). A conserved family of calcineurin regulators. Genes Dev. 14: 1595-1604 [Abstract] [Full Text]  
  • Conboy, M. J., Cyert, M. S. (2000). Luv1p/Rki1p/Tcs3p/Vps54p, a Yeast Protein That Localizes to the Late Golgi and Early Endosome, Is Required for Normal Vacuolar Morphology.. Mol. Biol. Cell 11: 2429-2443 [Abstract] [Full Text]  
  • Fu, L., Miseta, A., Hunton, D., Marchase, R. B., Bedwell, D. M. (2000). Loss of the Major Isoform of Phosphoglucomutase Results in Altered Calcium Homeostasis in Saccharomyces cerevisiae. J. Biol. Chem. 275: 5431-5440 [Abstract] [Full Text]  
  • Kucharczyk, R, Dupre, S, Avaro, S, Haguenauer-Tsapis, R, Slonimski, P., Rytka, J (2000). The novel protein Ccz1p required for vacuolar assembly in Saccharomyces cerevisiae functions in the same transport pathway as Ypt7p. J. Cell Sci. 113: 4301-4311 [Abstract]  
  • Yabe, I., Horiuchi, K.-i., Nakahara, K., Hiyama, T., Yamanaka, T., Wang, P.-C., Toda, K., Hirata, A., Ohsumi, Y., Hirata, R., Anraku, Y., Kusaka, I. (1999). Patch Clamp Studies on V-type ATPase of Vacuolar Membrane of Haploid Saccharomyces cerevisiae. PREPARATION AND UTILIZATION OF A GIANT CELL CONTAINING A GIANT VACUOLE. J. Biol. Chem. 274: 34903-34910 [Abstract] [Full Text]  
  • Alepuz, P. M., Matheos, D., Cunningham, K. W., Estruch, F. (1999). The Saccharomyces cerevisiae RanGTP-Binding Protein Msn5p Is Involved in Different Signal Transduction Pathways. Genetics 153: 1219-1231 [Abstract] [Full Text]  
  • Hirschi, K. D. (1999). Expression of Arabidopsis CAX1 in Tobacco: Altered Calcium Homeostasis and Increased Stress Sensitivity. Plant Cell 11: 2113-2122 [Abstract] [Full Text]  
  • de Nadal, E., Calero, F., Ramos, J., Ariño, J. (1999). Biochemical and Genetic Analyses of the Role of Yeast Casein Kinase 2 in Salt Tolerance. J. Bacteriol. 181: 6456-6462 [Abstract] [Full Text]  
  • Ungermann, C., Wickner, W., Xu, Z. (1999). Vacuole acidification is required for trans-SNARE pairing, LMA1 release, and homotypic fusion. Proc. Natl. Acad. Sci. USA 96: 11194-11199 [Abstract] [Full Text]  
  • Jiang, B., Cyert, M. S. (1999). Identification of a Novel Region Critical for Calcineurin Function in Vivo and in Vitro. J. Biol. Chem. 274: 18543-18551 [Abstract] [Full Text]  
  • Mulet, J. M., Leube, M. P., Kron, S. J., Rios, G., Fink, G. R., Serrano, R. (1999). A Novel Mechanism of Ion Homeostasis and Salt Tolerance in Yeast: the Hal4 and Hal5 Protein Kinases Modulate the Trk1-Trk2 Potassium Transporter. Mol. Cell. Biol. 19: 3328-3337 [Abstract] [Full Text]  
  • Stathopoulos-Gerontides, A., Guo, J. J., Cyert, M. S. (1999). Yeast calcineurin regulates nuclear localization of the Crz1p transcription factor through dephosphorylation. Genes Dev. 13: 798-803 [Abstract] [Full Text]  
  • Sanders, D., Brownlee, C., Harper, J. F. (1999). Communicating with Calcium. Plant Cell 11: 691-706 [Full Text]  
  • Piao, H. L., Tae Pih, K., Hwa Lim, J., Gene Kang, S., Bo Jin, J., Hee Kim, S., Hwang, I. (1999). An Arabidopsis GSK3/shaggy-Like Gene That Complements Yeast Salt Stress-Sensitive Mutants Is Induced by NaCl and Abscisic Acid. Plant Physiol. 119: 1527-1534 [Abstract] [Full Text]  
  • Miseta, A., Fu, L., Kellermayer, R., Buckley, J., Bedwell, D. M. (1999). The Golgi Apparatus Plays a Significant Role in the Maintenance of Ca2+ Homeostasis in the vps33Delta Vacuolar Biogenesis Mutant of Saccharomyces cerevisiae. J. Biol. Chem. 274: 5939-5947 [Abstract] [Full Text]  
  • Kraev, A., Kraev, N., Carafoli, E. (1999). Identification and Functional Expression of the Plasma Membrane Calcium ATPase Gene Family from Caenorhabditis elegans. J. Biol. Chem. 274: 4254-4258 [Abstract] [Full Text]  
  • Moniakis, J, Coukell, M., Janiec, A (1999). Involvement of the Ca2+-ATPase PAT1 and the contractile vacuole in calcium regulation in Dictyostelium discoideum. J. Cell Sci. 112: 405-414 [Abstract]  
  • Gustin, M. C., Albertyn, J., Alexander, M., Davenport, K. (1998). MAP Kinase Pathways in the Yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62: 1264-1300 [Abstract] [Full Text]  
  • Liang, F., Sze, H. (1998). A High-Affinity Ca2+ Pump, ECA1, from the Endoplasmic Reticulum Is Inhibited by Cyclopiazonic Acid but Not by Thapsigargin. Plant Physiol. 118: 817-825 [Abstract] [Full Text]  
  • Talla, E., de Mendonca, R. L., Degand, I., Goffeau, A., Ghislain, M. (1998). Schistosoma mansoni Ca2+-ATPase SMA2 Restores Viability to Yeast Ca2+-ATPase-deficient Strains and Functions in Calcineurin-mediated Ca2+ Tolerance. J. Biol. Chem. 273: 27831-27840 [Abstract] [Full Text]  
  • Ganster, R. W., McCartney, R. R., Schmidt, M. C. (1998). Identification of a Calcineurin-Independent Pathway Required for Sodium Ion Stress Response in Saccharomyces cerevisiae. Genetics 150: 31-42 [Abstract] [Full Text]  
  • Pardo, J. M., Reddy, M. P., Yang, S., Maggio, A., Huh, G.-H., Matsumoto, T., Coca, M. A., Paino-D'Urzo, M., Koiwa, H., Yun, D.-J., Watad, A. A., Bressan, R. A., Hasegawa, P. M. (1998). Stress signaling through Ca2+/calmodulin-dependent protein phosphatase calcineurin mediates salt adaptation in plants. Proc. Natl. Acad. Sci. USA 95: 9681-9686 [Abstract] [Full Text]  
  • Withee, J. L., Sen, R., Cyert, M. S. (1998). Ion Tolerance of Saccharomyces cerevisiae Lacking the Ca2+/CaM-Dependent Phosphatase (Calcineurin) Is Improved by Mutations in URE2 or PMA1. Genetics 149: 865-878 [Abstract] [Full Text]  
  • Masson, J.-Y., Ramotar, D. (1998). The Transcriptional Activator Imp2p Maintains Ion Homeostasis in Saccharomyces cerevisiae. Genetics 149: 893-901 [Abstract] [Full Text]  


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