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Mol. Cell. Biol., 05 1996, 2361-2368, Vol 16, No. 5
Copyright © 1996, American Society for Microbiology

Rad23 is required for transcription-coupled repair and efficient overrall repair in Saccharomyces cerevisiae

JP Mueller and MJ Smerdon
Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington 99164-4660, USA.

The repair of UV-induced photoproducts (cyclobutane pyrimidine dimers) in a well-characterized minichromosome, genomic DNA, and a transcribed genomic gene (RPB2) of a rad23delta mutant of Saccharomyces care was examined. Isogenic wild-type cells show a strong bias for the repair of the transcribed strands in both the plasmid and genomic genes and efficient overall repair of both DNAs (>80% of the dimers were removed in 6 h). However, the rad23delta mutant shows (i) no strand bias for repair in these genes and decreased repair of both strands, (ii) partial repair of genomic DNA (approximately 45% in 6 h), and (iii) very poor repair of the plasmid overall approximately 15% in 6 h). These features, coupled with the decreased UV survival of rad23delta cells, indicate that Rad23 is required for both transcription-coupled repair and efficient overall repair in S. cerevisiae.


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