This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huet, X.
Right arrow Articles by Blanchard, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huet, X.
Right arrow Articles by Blanchard, J. M.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Jul 1996, 3789-3798, Vol 16, No. 7
Copyright © 1996, American Society for Microbiology

Cyclin A expression is under negative transcriptional control during the cell cycle

X Huet, J Rech, A Plet, A Vie and JM Blanchard
Institut de Genetique Moleculaire de Montpellier, CNRS UMR 5535, France.

Transcription of the gene coding for cyclin A, a protein required for S- phase transit, is cell cycle regulated and is restricted to proliferating cells. To further explore transcriptional regulation linked to cell division cycle control, a genomic clone containing 5' flanking sequences of the murine cyclin A gene was isolated. When it was fused to a luciferase reporter gene, it was shown to function as a proliferation-regulated promoter in NIH 3T3 cells. Transcription of the mouse cyclin A gene is negatively regulated by arrest of cell proliferation. A mutation of a GC-rich sequence conserved between mice and humans is sufficient to relieve transcriptional repression, resulting in a promoter with constitutively high activity. In agreement with this result, in vivo footprinting reveals a protection of the cell cycle-responsive element in G0/early G1 cells which is not observed at later stages of the cell cycle. Moreover, the footprint is present in dimethyl sulfoxide-induced differentiating and not in proliferating Friend erythroleukemia cells. Conversely, two other sites, which in vitro bind ATF-1 and NF-Y, respectively, are constitutively occupied throughout cell cycle progression.


This article has been cited by other articles:

  • Walker, A., Acquaviva, C., Matsusaka, T., Koop, L., Pines, J. (2008). UbcH10 has a rate-limiting role in G1 phase but might not act in the spindle checkpoint or as part of an autonomous oscillator. J. Cell Sci. 121: 2319-2326 [Abstract] [Full Text]  
  • Benatti, P., Basile, V., Merico, D., Fantoni, L. I., Tagliafico, E., Imbriano, C. (2008). A balance between NF-Y and p53 governs the pro- and anti-apoptotic transcriptional response. Nucleic Acids Res 36: 1415-1428 [Abstract] [Full Text]  
  • Myslinski, E., Gerard, M.-A., Krol, A., Carbon, P. (2007). Transcription of the human cell cycle regulated BUB1B gene requires hStaf/ZNF143. Nucleic Acids Res 35: 3453-3464 [Abstract] [Full Text]  
  • Yoshizumi, T., Tsumoto, Y., Takiguchi, T., Nagata, N., Yamamoto, Y. Y., Kawashima, M., Ichikawa, T., Nakazawa, M., Yamamoto, N., Matsui, M. (2006). INCREASED LEVEL OF POLYPLOIDY1, a Conserved Repressor of CYCLINA2 Transcription, Controls Endoreduplication in Arabidopsis. Plant Cell 18: 2452-2468 [Abstract] [Full Text]  
  • Parakati, R., DiMario, J. X. (2005). Dynamic Transcriptional Regulatory Complexes, Including E2F4, p107, p130, and Sp1, Control Fibroblast Growth Factor Receptor 1 Gene Expression during Myogenesis. J. Biol. Chem. 280: 21284-21294 [Abstract] [Full Text]  
  • Ahmed-Choudhury, J., Agathanggelou, A., Fenton, S. L., Ricketts, C., Clark, G. J., Maher, E. R., Latif, F. (2005). Transcriptional Regulation of Cyclin A2 by RASSF1A through the Enhanced Binding of p120E4F to the Cyclin A2 Promoter. Cancer Res. 65: 2690-2697 [Abstract] [Full Text]  
  • Tessari, M. A., Gostissa, M., Altamura, S., Sgarra, R., Rustighi, A., Salvagno, C., Caretti, G., Imbriano, C., Mantovani, R., Del Sal, G., Giancotti, V., Manfioletti, G. (2003). Transcriptional Activation of the Cyclin A Gene by the Architectural Transcription Factor HMGA2. Mol. Cell. Biol. 23: 9104-9116 [Abstract] [Full Text]  
  • Siddiqui, H., Solomon, D. A., Gunawardena, R. W., Wang, Y., Knudsen, E. S. (2003). Histone Deacetylation of RB-Responsive Promoters: Requisite for Specific Gene Repression but Dispensable for Cell Cycle Inhibition. Mol. Cell. Biol. 23: 7719-7731 [Abstract] [Full Text]  
  • Gurtner, A., Manni, I., Fuschi, P., Mantovani, R., Guadagni, F., Sacchi, A., Piaggio, G. (2003). Requirement for Down-Regulation of the CCAAT-binding Activity of the NF-Y Transcription Factor during Skeletal Muscle Differentiation. Mol. Biol. Cell 14: 2706-2715 [Abstract] [Full Text]  
  • Zhou, Y., Mehta, K. R., Choi, A. P., Scolavino, S., Zhang, X. (2003). DNA Damage-induced Inhibition of Securin Expression Is Mediated by p53. J. Biol. Chem. 278: 462-470 [Abstract] [Full Text]  
  • Andrecht, S., Kolbus, A., Hartenstein, B., Angel, P., Schorpp-Kistner, M. (2002). Cell Cycle Promoting Activity of JunB through Cyclin A Activation. J. Biol. Chem. 277: 35961-35968 [Abstract] [Full Text]  
  • Buchanan, D. L., Ohsako, S., Tohyama, C., Cooke, P. S., Iguchi, T. (2002). Dioxin Inhibition of Estrogen-Induced Mouse Uterine Epithelial Mitogenesis Involves Changes in Cyclin and Transforming Growth Factor-{beta} Expression. Toxicol Sci 66: 62-68 [Abstract] [Full Text]  
  • Bottazzi, M. E., Buzzai, M., Zhu, X., Desdouets, C., Brechot, C., Assoian, R. K. (2001). Distinct Effects of Mitogens and the Actin Cytoskeleton on CREB and Pocket Protein Phosphorylation Control the Extent and Timing of Cyclin A Promoter Activity. Mol. Cell. Biol. 21: 7607-7616 [Abstract] [Full Text]  
  • Schuchner, S., Nemethova, M., Belisova, A., Klucky, B., Holnthoner, W., Wintersberger, E. (2001). Transactivation of Murine Cyclin A by Polyomavirus Large and Small T Antigens. J. Virol. 75: 6498-6507 [Abstract] [Full Text]  
  • Pestov, D. G., Strezoska, Z., Lau, L. F. (2001). Evidence of p53-Dependent Cross-Talk between Ribosome Biogenesis and the Cell Cycle: Effects of Nucleolar Protein Bop1 on G1/S Transition. Mol. Cell. Biol. 21: 4246-4255 [Abstract] [Full Text]  
  • Fajas, L., Paul, C., Vié, A., Estrach, S., Medema, R., Blanchard, J. M., Sardet, C., Vignais, M.-L. (2001). Cyclin A Is a Mediator of p120E4F-Dependent Cell Cycle Arrest in G1. Mol. Cell. Biol. 21: 2956-2966 [Abstract] [Full Text]  
  • Takahashi, Y., Rayman, J. B., Dynlacht, B. D. (2000). Analysis of promoter binding by the E2F and pRB families in vivo: distinct E2F proteins mediate activation and repression. Genes Dev. 14: 804-816 [Abstract] [Full Text]  
  • Koziczak, M., Krek, W., Nagamine, Y. (2000). Pocket Protein-Independent Repression of Urokinase-Type Plasminogen Activator and Plasminogen Activator Inhibitor 1 Gene Expression by E2F1. Mol. Cell. Biol. 20: 2014-2022 [Abstract] [Full Text]  
  • Humbert, P. O., Verona, R., Trimarchi, J. M., Rogers, C., Dandapani, S., Lees, J. A. (2000). E2f3 is critical for normal cellular proliferation. Genes Dev. 14: 690-703 [Abstract] [Full Text]  
  • Rivard, A., Principe, N., Andres, V. (2000). Age-dependent increase in c-fos activity and cyclin A expression in vascular smooth muscle cells: A potential link between aging, smooth muscle cell proliferation and atherosclerosis. Cardiovasc Res 45: 1026-1034 [Abstract] [Full Text]  
  • Schuchner, S., Wintersberger, E. (1999). Binding of Polyomavirus Small T Antigen to Protein Phosphatase 2A Is Required for Elimination of p27 and Support of S-Phase Induction in Concert with Large T Antigen. J. Virol. 73: 9266-9273 [Abstract] [Full Text]  
  • Knudsen, K. E., Fribourg, A. F., Strobeck, M. W., Blanchard, J.-M., Knudsen, E. S. (1999). Cyclin A Is a Functional Target of Retinoblastoma Tumor Suppressor Protein-mediated Cell Cycle Arrest. J. Biol. Chem. 274: 27632-27641 [Abstract] [Full Text]  
  • Vigo, E., Muller, H., Prosperini, E., Hateboer, G., Cartwright, P., Moroni, M. C., Helin, K. (1999). CDC25A Phosphatase Is a Target of E2F and Is Required for Efficient E2F-Induced S Phase. Mol. Cell. Biol. 19: 6379-6395 [Abstract] [Full Text]  
  • Pestell, R. G., Albanese, C., Reutens, A. T., Segall, J. E., Lee, R. J., Arnold, A. (1999). The Cyclins and Cyclin-Dependent Kinase Inhibitors in Hormonal Regulation of Proliferation and Differentiation. Endocr. Rev. 20: 501-534 [Abstract] [Full Text]  
  • van der Sman, J., Thomas, N. S. B., Lam, E. W.-F. (1999). Modulation of E2F Complexes during G0 to S Phase Transition in Human Primary B-lymphocytes. J. Biol. Chem. 274: 12009-12016 [Abstract] [Full Text]  
  • Sibinga, N. E. S., Wang, H., Perrella, M. A., Endege, W. O., Patterson, C., Yoshizumi, M., Haber, E., Lee, M.-E. (1999). Interferon-{gamma}-mediated Inhibition of Cyclin A Gene Transcription Is Independent of Individual cis-Acting Elements in the Cyclin A Promoter. J. Biol. Chem. 274: 12139-12146 [Abstract] [Full Text]  
  • Muller, C., Yang, R., Beck-von-Peccoz, L., Idos, G., Verbeek, W., Koeffler, H. P. (1999). Cloning of the cyclin A1 Genomic Structure and Characterization of the Promoter Region. GC BOXES ARE ESSENTIAL FOR CELL CYCLE-REGULATED TRANSCRIPTION OF THE cyclin A1 GENE. J. Biol. Chem. 274: 11220-11228 [Abstract] [Full Text]  
  • Ravi, R. K., Thiagalingam, A., Weber, E., McMahon, M., Nelkin, B. D., Mabry, M. (1999). Raf-1 Causes Growth Suppression and Alteration of Neuroendocrine Markers in DMS53 Human Small-Cell Lung Cancer Cells. Am. J. Respir. Cell Mol. Bio. 20: 543-549 [Abstract] [Full Text]  
  • Davey, G, Buzzai, M, Assoian, R. (1999). Reduced expression of (alpha)5(beta)1 integrin prevents spreading-dependent cell proliferation. J. Cell Sci. 112: 4663-4672 [Abstract]  
  • Iavarone, A., Massague, J. (1999). E2F and Histone Deacetylase Mediate Transforming Growth Factor beta  Repression of cdc25A during Keratinocyte Cell Cycle Arrest. Mol. Cell. Biol. 19: 916-922 [Abstract] [Full Text]  
  • Nayler, O., Schnorrer, F., Stamm, S., Ullrich, A. (1998). The Cellular Localization of the Murine Serine/Arginine-rich Protein Kinase CLK2 Is Regulated by Serine 141 Autophosphorylation. J. Biol. Chem. 273: 34341-34348 [Abstract] [Full Text]  
  • LeCouter, J. E., Kablar, B., Hardy, W. R., Ying, C., Megeney, L. A., May, L. L., Rudnicki, M. A. (1998). Strain-Dependent Myeloid Hyperplasia, Growth Deficiency, and Accelerated Cell Cycle in Mice Lacking the Rb-Related p107 Gene. Mol. Cell. Biol. 18: 7455-7465 [Abstract] [Full Text]  
  • Hateboer, G., Wobst, A., Petersen, B. O., Le Cam, L., Vigo, E., Sardet, C., Helin, K. (1998). Cell Cycle-Regulated Expression of Mammalian CDC6 Is Dependent on E2F. Mol. Cell. Biol. 18: 6679-6697 [Abstract] [Full Text]  
  • Dyson, N. (1998). The regulation of E2F by pRB-family proteins. Genes Dev. 12: 2245-2262 [Full Text]  
  • Cheng, M., Sexl, V., Sherr, C. J., Roussel, M. F. (1998). Assembly of cyclin D-dependent kinase and titration of p27Kip1 regulated by mitogen-activated protein kinase kinase (MEK1). Proc. Natl. Acad. Sci. USA 95: 1091-1096 [Abstract] [Full Text]  
  • Mulligan, G. J., Wong, J., Jacks, T. (1998). p130 Is Dispensable in Peripheral T Lymphocytes: Evidence for Functional Compensation by p107 and pRB. Mol. Cell. Biol. 18: 206-220 [Abstract] [Full Text]  
  • Leveillard, T., Wasylyk, B. (1997). The MDM2 C-terminal Region Binds to TAFII250 and Is Required for MDM2 Regulation of the Cyclin A Promoter. J. Biol. Chem. 272: 30651-30661 [Abstract] [Full Text]  
  • Yoshizumi, M., Wang, H., Hsieh, C.-M., Sibinga, N. E. S., Perrella, M. A., Lee, M.-E. (1997). Down-regulation of the Cyclin A Promoter by Transforming Growth Factor-beta 1 Is Associated with a Reduction in Phosphorylated Activating Transcription Factor-1 and Cyclic AMP-responsive Element-binding Protein. J. Biol. Chem. 272: 22259-22264 [Abstract] [Full Text]  
  • Hurford, R K, Cobrinik, D, Lee, M H, Dyson, N (1997). pRB and p107/p130 are required for the regulated expression of different sets of E2F responsive genes.. Genes Dev. 11: 1447-1463 [Abstract]  
  • Pumiglia, K. M., Decker, S. J. (1997). Cell cycle arrest mediated by the MEK/mitogen-activated protein kinase pathway. Proc. Natl. Acad. Sci. USA 94: 448-452 [Abstract] [Full Text]  
  • Assoian, R. K. (1997). Anchorage-dependent Cell Cycle Progression. JCB 136: 1-4 [Full Text]  
  • Paramio, J. M., Segrelles, C., Casanova, M. L., Jorcano, J. L. (2000). Opposite Functions for E2F1 and E2F4 in Human Epidermal Keratinocyte Differentiation. J. Biol. Chem. 275: 41219-41226 [Abstract] [Full Text]  
  • Kaufmann, H., Marone, R., Olayioye, M. A., Bailey, J. E., Fussenegger, M. (2001). Characterization of an N-terminally Truncated Cyclin A Isoform in Mammalian Cells. J. Biol. Chem. 276: 29987-29993 [Abstract] [Full Text]  
  • Korner, K., Jerome, V., Schmidt, T., Muller, R. (2001). Cell Cycle Regulation of the Murine cdc25B Promoter. ESSENTIAL ROLE FOR NUCLEAR FACTOR-Y AND A PROXIMAL REPRESSOR ELEMENT. J. Biol. Chem. 276: 9662-9669 [Abstract] [Full Text]  
  • Tarn, C., Lee, S., Hu, Y., Ashendel, C., Andrisani, O. M. (2001). Hepatitis B Virus X Protein Differentially Activates RAS-RAF-MAPK and JNK Pathways in X-transforming Versus Non-transforming AML12 Hepatocytes. J. Biol. Chem. 276: 34671-34680 [Abstract] [Full Text]  
  • Sanchez, T., Moreno, J. J. (2002). Role of EP1 and EP4 PGE2 subtype receptors in serum-induced 3T6 fibroblast cycle progression and proliferation. Am. J. Physiol. Cell Physiol. 282: C280-C288 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»