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Mol. Cell. Biol., 10 1997, 5719-5726, Vol 17, No. 10
Copyright © 1997, American Society for Microbiology

Endothelial cytosolic proteins bind to the 3' untranslated region of endothelial nitric oxide synthase mRNA: regulation by tumor necrosis factor alpha

J Alonso, L Sanchez de Miguel, M Monton, S Casado and A Lopez-Farre
Nephrology, Hypertension and Cardiovascular Research Laboratory, Instituto de Investigaciones Medicas, Fundacion Jimenez Diaz, Madrid, Spain.

Changes in endothelial nitric oxide synthase (eNOS) expression may be involved in the endothelium-dependent vasorelaxation dysfunction associated with several vascular diseases. In the present work, we demonstrate that eNOS mRNA contains a previously undescribed cis element in the 3' untranslated region (3' UTR). A U+C-rich segment in the 3' UTR is critical in complex formation with bovine aortic endothelial cell cytosolic proteins. Tumor necrosis factor alpha (TNF- alpha), which destabilizes eNOS mRNA, increased the binding activity of the cytosolic proteins in a time-dependent manner. These data suggest that endothelial cytosolic proteins bind to the 3' UTR of eNOS mRNA. These proteins may play a role in TNF-alpha-induced eNOS mRNA destabilization.


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