This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Logan, S. K.
Right arrow Articles by Schlessinger, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Logan, S. K.
Right arrow Articles by Schlessinger, J.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 10 1997, 5784-5790, Vol 17, No. 10
Copyright © 1997, American Society for Microbiology

Phosphatidylinositol 3-kinase mediates epidermal growth factor-induced activation of the c-Jun N-terminal kinase signaling pathway

SK Logan, M Falasca, P Hu and J Schlessinger
Department of Pharmacology, New York University Medical Center, New York 10016, USA.

The signaling events which mediate activation of c-Jun N-terminal kinase (JNK) are not yet well characterized. To broaden our understanding of upstream mediators which link extracellular signals to the JNK pathway, we investigated the role of phosphatidylinositol (PI) 3-kinase in epidermal growth factor (EGF)-mediated JNK activation. In this report we demonstrate that a dominant negative form of PI 3-kinase as well as the inhibitor wortmannin blocks EGF-induced JNK activation dramatically. However, wortmannin does not have an effect on JNK activation induced by UV irradiation or osmotic shock. In addition, a membrane-targeted, constitutively active PI 3-kinase (p110beta) was shown to produce in vivo products and to activate JNK, while a kinase- mutated form of this protein showed no activation. On the basis of these experiments, we propose that PI 3-kinase activity plays a role in EGF-induced JNK activation in these cells.


This article has been cited by other articles:

  • Endo, Y., Sugiyama, A., Li, S.-A., Ohmori, K., Ohata, H., Yoshida, Y., Shibuya, M., Takei, K., Enari, M., Taya, Y. (2008). Regulation of clathrin-mediated endocytosis by p53.. GENES CELLS 13: 375-386 [Abstract] [Full Text]  
  • Frazier, D. P., Wilson, A., Dougherty, C. J., Li, H., Bishopric, N. H., Webster, K. A. (2007). PKC-{alpha} and TAK-1 are intermediates in the activation of c-Jun NH2-terminal kinase by hypoxia-reoxygenation. Am. J. Physiol. Heart Circ. Physiol. 292: H1675-H1684 [Abstract] [Full Text]  
  • Oliva, A. A. Jr, Atkins, C. M., Copenagle, L., Banker, G. A. (2006). Activated c-Jun N-Terminal Kinase Is Required for Axon Formation.. J. Neurosci. 26: 9462-9470 [Abstract] [Full Text]  
  • Taneja, S. S., Ha, S., Swenson, N. K., Huang, H. Y., Lee, P., Melamed, J., Shapiro, E., Garabedian, M. J., Logan, S. K. (2005). Cell-specific Regulation of Androgen Receptor Phosphorylation in Vivo. J. Biol. Chem. 280: 40916-40924 [Abstract] [Full Text]  
  • DOUGHERTY, C. J., KUBASIAK, L. A., FRAZIER, D. P., LI, H., XIONG, W.-CHENG., BISHOPRIC, N. H., WEBSTER, K. A. (2004). Mitochondrial signals initiate the activation of c-Jun N-terminal kinase (JNK) by hypoxia-reoxygenation. FASEB J. 18: 1060-1070 [Abstract] [Full Text]  
  • Arndt, P. G., Suzuki, N., Avdi, N. J., Malcolm, K. C., Worthen, G. S. (2004). Lipopolysaccharide-induced c-Jun NH2-terminal Kinase Activation in Human Neutrophils: ROLE OF PHOSPHATIDYLINOSITOL 3-KINASE AND Syk-MEDIATED PATHWAYS. J. Biol. Chem. 279: 10883-10891 [Abstract] [Full Text]  
  • Du, L., Lyle, C. S., Obey, T. B., Gaarde, W. A., Muir, J. A., Bennett, B. L., Chambers, T. C. (2004). Inhibition of Cell Proliferation and Cell Cycle Progression by Specific Inhibition of Basal JNK Activity: EVIDENCE THAT MITOTIC Bcl-2 PHOSPHORYLATION IS JNK-INDEPENDENT. J. Biol. Chem. 279: 11957-11966 [Abstract] [Full Text]  
  • Bose, C., Bhuvaneswaran, C., Udupa, K. B. (2004). Altered Mitogen-Activated Protein Kinase Signal Transduction in Human Skin Fibroblasts During In Vitro Aging: Differential Expression of Low-Density Lipoprotein Receptor. Journals of Gerontology Series A: Biological Sciences and Medical Sciences 59: B126-135 [Abstract] [Full Text]  
  • Yang, Y.-M., Bost, F., Charbono, W., Dean, N., McKay, R., Rhim, J. S., Depatie, C., Mercola, D. (2003). C-Jun NH2-terminal Kinase Mediates Proliferation and Tumor Growth of Human Prostate Carcinoma. Clin. Cancer Res. 9: 391-401 [Abstract] [Full Text]  
  • Pedram, A., Razandi, M., Levin, E. R. (2002). Deciphering Vascular Endothelial Cell Growth Factor/Vascular Permeability Factor Signaling to Vascular Permeability. INHIBITION BY ATRIAL NATRIURETIC PEPTIDE. J. Biol. Chem. 277: 44385-44398 [Abstract] [Full Text]  
  • Lutz, C., Nimpf, J., Jenny, M., Boecklinger, K., Enzinger, C., Utermann, G., Baier-Bitterlich, G., Baier, G. (2002). Evidence of Functional Modulation of the MEKK/JNK/cJun Signaling Cascade by the Low Density Lipoprotein Receptor-related Protein (LRP). J. Biol. Chem. 277: 43143-43151 [Abstract] [Full Text]  
  • Seta, K. A., Spicer, Z., Yuan, Y., Lu, G., Millhorn, D. E. (2002). Responding to Hypoxia: Lessons From a Model Cell Line. Sci Signal 2002: re11-re11 [Abstract] [Full Text]  
  • Yuan, Z.-q., Feldman, R. I., Sun, M., Olashaw, N. E., Coppola, D., Sussman, G. E., Shelley, S. A., Nicosia, S. V., Cheng, J. Q. (2002). Inhibition of JNK by Cellular Stress- and Tumor Necrosis Factor alpha -induced AKT2 through Activation of the NFkappa B Pathway in Human Epithelial Cells. J. Biol. Chem. 277: 29973-29982 [Abstract] [Full Text]  
  • Dehez, S., Bierkamp, C., Kowalski-Chauvel, A., Daulhac, L., Escrieut, C., Susini, C., Pradayrol, L., Fourmy, D., Seva, C. (2002). c-Jun NH2-terminal Kinase Pathway in Growth-promoting Effect of the G Protein-coupled Receptor Cholecystokinin B Receptor: A Protein Kinase C/Src-dependent-Mechanism. Cell Growth Differ. 13: 375-385 [Abstract] [Full Text]  
  • Berlanga, J., Prats, P., Remirez, D., Gonzalez, R., Lopez-Saura, P., Aguiar, J., Ojeda, M., Boyle, J. J., Fitzgerald, A. J., Playford, R. J. (2002). Prophylactic Use of Epidermal Growth Factor Reduces Ischemia/Reperfusion Intestinal Damage. Am. J. Pathol. 161: 373-379 [Abstract] [Full Text]  
  • Ming, X.-F., Stoecklin, G., Lu, M., Looser, R., Moroni, C. (2001). Parallel and Independent Regulation of Interleukin-3 mRNA Turnover by Phosphatidylinositol 3-Kinase and p38 Mitogen-Activated Protein Kinase. Mol. Cell. Biol. 21: 5778-5789 [Abstract] [Full Text]  
  • Morino, K., Maegawa, H., Fujita, T., Takahara, N., Egawa, K., Kashiwagi, A., Kikkawa, R. (2001). Insulin-Induced c-Jun N-Terminal Kinase Activation Is Negatively Regulated by Protein Kinase C {{delta}}. Endocrinology 142: 2669-2676 [Abstract] [Full Text]  
  • Lee, Z. H., Kwack, K., Kim, K. K., Lee, S. H., Kim, H.-H. (2001). Activation of c-Jun N-Terminal Kinase and Activator Protein 1 by Receptor Activator of Nuclear Factor kappa B. Mol. Pharmacol. 58: 1536-1545 [Abstract] [Full Text]  
  • Ellerbroek, S. M., Halbleib, J. M., Benavidez, M., Warmka, J. K., Wattenberg, E. V., Stack, M. S., Hudson, L. G. (2001). Phosphatidylinositol 3-Kinase Activity in Epidermal Growth Factor-stimulated Matrix Metalloproteinase-9 Production and Cell Surface Association. Cancer Res. 61: 1855-1861 [Abstract] [Full Text]  
  • Caballero, M E, Berlanga, J, Ramirez, D, Lopez-Saura, P, Gozalez, R, Floyd, D N, Marchbank, T, Playford, R J (2001). Epidermal growth factor reduces multiorgan failure induced by thioacetamide. Gut 48: 34-40 [Abstract] [Full Text]  
  • Procyk, K. J., Rippo, M. R., Testi, R., Hofmann, F., Parker, P. J., Baccarini, M. (2000). Lipopolysaccharide induces Jun N-terminal kinase activation in macrophages by a novel Cdc42/Rac-independent pathway involving sequential activation of protein kinase C zeta and phosphatidylcholine-dependent phospholipase C. Blood 96: 2592-2598 [Abstract] [Full Text]  
  • Jacobs-Helber, S. M., Ryan, J. J., Sawyer, S. T. (2000). JNK and p38 are activated by erythropoietin (EPO) but are not induced in apoptosis following EPO withdrawal in EPO-dependent HCD57 cells. Blood 96: 933-940 [Abstract] [Full Text]  
  • Wooten, M. W., Seibenhener, M. L., Neidigh, K. B. W., Vandenplas, M. L. (2000). Mapping of Atypical Protein Kinase C within the Nerve Growth Factor Signaling Cascade: Relationship to Differentiation and Survival of PC12 Cells. Mol. Cell. Biol. 20: 4494-4504 [Abstract] [Full Text]  
  • Razzini, G., Ingrosso, A., Brancaccio, A., Sciacchitano, S., Esposito, D. L., Falasca, M. (2000). Different Subcellular Localization and Phosphoinositides Binding of Insulin Receptor Substrate Protein Pleckstrin Homology Domains. Mol. Endocrinol. 14: 823-836 [Abstract] [Full Text]  
  • Fukunaga, K., Noguchi, T., Takeda, H., Matozaki, T., Hayashi, Y., Itoh, H., Kasuga, M. (2000). Requirement for Protein-tyrosine Phosphatase SHP-2 in Insulin-induced Activation of c-Jun NH2-terminal Kinase. J. Biol. Chem. 275: 5208-5213 [Abstract] [Full Text]  
  • Rodrigues, G. A., Falasca, M., Zhang, Z., Ong, S. H., Schlessinger, J. (2000). A Novel Positive Feedback Loop Mediated by the Docking Protein Gab1 and Phosphatidylinositol 3-Kinase in Epidermal Growth Factor Receptor Signaling. Mol. Cell. Biol. 20: 1448-1459 [Abstract] [Full Text]  
  • Monno, S., Newman, M. V., Cook, M., Lowe, W. L. Jr. (2000). Insulin-Like Growth Factor I Activates c-Jun N-Terminal Kinase in MCF-7 Breast Cancer Cells. Endocrinology 141: 544-550 [Abstract] [Full Text]  
  • Lu, Z., Hornia, A., Joseph, T., Sukezane, T., Frankel, P., Zhong, M., Bychenok, S., Xu, L., Feig, L. A., Foster, D. A. (2000). Phospholipase D and RalA Cooperate with the Epidermal Growth Factor Receptor To Transform 3Y1 Rat Fibroblasts. Mol. Cell. Biol. 20: 462-467 [Abstract] [Full Text]  
  • Jiang, K., Coppola, D., Crespo, N. C., Nicosia, S. V., Hamilton, A. D., Sebti, S. M., Cheng, J. Q. (2000). The Phosphoinositide 3-OH Kinase/AKT2 Pathway as a Critical Target for Farnesyltransferase Inhibitor-Induced Apoptosis. Mol. Cell. Biol. 20: 139-148 [Abstract] [Full Text]  
  • Hayes, A. L., Smith, C., Foxwell, B. M. J., Brennan, F. M. (1999). CD45-induced Tumor Necrosis Factor alpha Production in Monocytes Is Phosphatidylinositol 3-Kinase-dependent and Nuclear Factor-kappa B-independent. J. Biol. Chem. 274: 33455-33461 [Abstract] [Full Text]  
  • Procyk, K. J., Rippo, M. R., Testi, R., Hoffmann, F., Parker, P. J., Baccarini, M. (1999). Distinct Mechanisms Target Stress and Extracellular Signal-Activated Kinase 1 and Jun N-Terminal Kinase During Infection of Macrophages with Salmonella. J. Immunol. 163: 4924-4930 [Abstract] [Full Text]  
  • Yu, R., Lei, W., Mandlekar, S., Weber, M. J., Der, C. J., Wu, J., Kong, A.-N. T. (1999). Role of a Mitogen-activated Protein Kinase Pathway in the Induction of Phase II Detoxifying Enzymes by Chemicals. J. Biol. Chem. 274: 27545-27552 [Abstract] [Full Text]  
  • Tiganis, T., Kemp, B. E., Tonks, N. K. (1999). The Protein-tyrosine Phosphatase TCPTP Regulates Epidermal Growth Factor Receptor-mediated and Phosphatidylinositol 3-Kinase-dependent Signaling. J. Biol. Chem. 274: 27768-27775 [Abstract] [Full Text]  
  • Kim, B.-C., Lee, M.-N., Kim, J.-Y., Lee, S.-S., Chang, J.-D., Kim, S.-S., Lee, S.-Y., Kim, J.-H. (1999). Roles of Phosphatidylinositol 3-Kinase and Rac in the Nuclear Signaling by Tumor Necrosis Factor-alpha in Rat-2 Fibroblasts. J. Biol. Chem. 274: 24372-24377 [Abstract] [Full Text]  
  • Conrad, P. W., Rust, R. T., Han, J., Millhorn, D. E., Beitner-Johnson, D. (1999). Selective Activation of p38alpha and p38gamma by Hypoxia. ROLE IN REGULATION OF CYCLIN D1 BY HYPOXIA IN PC12 CELLS. J. Biol. Chem. 274: 23570-23576 [Abstract] [Full Text]  
  • Hashimoto, A., Kurosaki, M., Gotoh, N., Shibuya, M., Kurosaki, T. (1999). Shc Regulates Epidermal Growth Factor-induced Activation of the JNK Signaling Pathway. J. Biol. Chem. 274: 20139-20143 [Abstract] [Full Text]  
  • Takata, M., Ogawa, W., Kitamura, T., Hino, Y., Kuroda, S., Kotani, K., Klip, A., Gingras, A.-C., Sonenberg, N., Kasuga, M. (1999). Requirement for Akt (Protein Kinase B) in Insulin-induced Activation of Glycogen Synthase and Phosphorylation of 4E-BP1 (PHAS-1). J. Biol. Chem. 274: 20611-20618 [Abstract] [Full Text]  
  • Oktay, M., Wary, K. K., Dans, M., Birge, R. B., Giancotti, F. G. (1999). Integrin-mediated Activation of Focal Adhesion Kinase Is Required for Signaling to Jun NH2-terminal Kinase and Progression through the G1 Phase of the Cell Cycle. JCB 145: 1461-1470 [Abstract] [Full Text]  
  • Moriguchi, Y., Matsubara, H., Mori, Y., Murasawa, S., Masaki, H., Maruyama, K., Tsutsumi, Y., Shibasaki, Y., Tanaka, Y., Nakajima, T., Oda, K., Iwasaka, T. (1999). Angiotensin II–Induced Transactivation of Epidermal Growth Factor Receptor Regulates Fibronectin and Transforming Growth Factor-ß Synthesis via Transcriptional and Posttranscriptional Mechanisms. Circ. Res. 84: 1073-1084 [Abstract] [Full Text]  
  • Jacob, A., Cooney, D., Tridandapani, S., Kelley, T., Coggeshall, K. M. (1999). Fcgamma RIIb Modulation of Surface Immunoglobulin-induced Akt Activation in Murine B Cells. J. Biol. Chem. 274: 13704-13710 [Abstract] [Full Text]  
  • Palmer, H. J., Tuzon, C. T., Paulson, K. E. (1999). Age-dependent Decline in Mitogenic Stimulation of Hepatocytes. REDUCED ASSOCIATION BETWEEN Shc AND THE EPIDERMAL GROWTH FACTOR RECEPTOR IS COUPLED TO DECREASED ACTIVATION OF Raf AND EXTRACELLULAR SIGNAL-REGULATED KINASES. J. Biol. Chem. 274: 11424-11430 [Abstract] [Full Text]  
  • Lu, Y., Brush, J., Stewart, T. A. (1999). NSP1 Defines a Novel Family of Adaptor Proteins Linking Integrin and Tyrosine Kinase Receptors to the c-Jun N-terminal Kinase/Stress-activated Protein Kinase Signaling Pathway. J. Biol. Chem. 274: 10047-10052 [Abstract] [Full Text]  
  • Lee, H.-Y., Sueoka, N., Hong, W.-K., Mangelsdorf, D. J., Claret, F. X., Kurie, J. M. (1999). All-trans-Retinoic Acid Inhibits Jun N-Terminal Kinase by Increasing Dual-Specificity Phosphatase Activity. Mol. Cell. Biol. 19: 1973-1980 [Abstract] [Full Text]  
  • Santiago, F. S., Lowe, H. C., Day, F. L., Chesterman, C. N., Khachigian, L. M. (1999). Early Growth Response Factor-1 Induction by Injury Is Triggered by Release and Paracrine Activation by Fibroblast Growth Factor-2. Am. J. Pathol. 154: 937-944 [Abstract] [Full Text]  
  • Takuwa, N., Fukui, Y., Takuwa, Y. (1999). Cyclin D1 Expression Mediated by Phosphatidylinositol 3-Kinase through mTOR-p70S6K-Independent Signaling in Growth Factor-Stimulated NIH 3T3 Fibroblasts. Mol. Cell. Biol. 19: 1346-1358 [Abstract] [Full Text]  
  • Johnston, S. R. D., Lu, B., Scott, G. K., Kushner, P. J., Smith, I. E., Dowsett, M., Benz, C. C. (1999). Increased Activator Protein-1 DNA Binding and c-Jun NH2-Terminal Kinase Activity in Human Breast Tumors with Acquired Tamoxifen Resistance. Clin. Cancer Res. 5: 251-256 [Abstract] [Full Text]  
  • Roche, S., Downward, J., Raynal, P., Courtneidge, S. A. (1998). A Function for Phosphatidylinositol 3-Kinase beta  (p85alpha -p110beta ) in Fibroblasts during Mitogenesis: Requirement for Insulin- and Lysophosphatidic Acid-Mediated Signal Transduction. Mol. Cell. Biol. 18: 7119-7129 [Abstract] [Full Text]  
  • Kavran, J. M., Klein, D. E., Lee, A., Falasca, M., Isakoff, S. J., Skolnik, E. Y., Lemmon, M. A. (1998). Specificity and Promiscuity in Phosphoinositide Binding by Pleckstrin Homology Domains. J. Biol. Chem. 273: 30497-30508 [Abstract] [Full Text]  
  • Okubo, Y., Blakesley, V. A., Stannard, B., Gutkind, S., Le Roith, D. (1998). Insulin-like Growth Factor-I Inhibits the Stress-activated Protein Kinase/c-Jun N-terminal Kinase. J. Biol. Chem. 273: 25961-25966 [Abstract] [Full Text]  
  • Pomerance, M., Multon, M.-C., Parker, F., Venot, C., Blondeau, J.-P., Tocque, B., Schweighoffer, F. (1998). Grb2 Interaction with MEK-Kinase 1 Is Involved in Regulation of Jun-Kinase Activities in Response to Epidermal Growth Factor. J. Biol. Chem. 273: 24301-24304 [Abstract] [Full Text]  
  • Madge, L. A., Pober, J. S. (2000). A Phosphatidylinositol 3-Kinase/Akt Pathway, Activated by Tumor Necrosis Factor or Interleukin-1, Inhibits Apoptosis but Does Not Activate NFkappa B in Human Endothelial Cells. J. Biol. Chem. 275: 15458-15465 [Abstract] [Full Text]  
  • Roig, J., Huang, Z., Lytle, C., Traugh, J. A. (2000). p21-activated Protein Kinase gamma -PAK Is Translocated and Activated in Response to Hyperosmolarity. IMPLICATION OF Cdc42 AND PHOSPHOINOSITIDE 3-KINASE IN A TWO-STEP MECHANISM FOR gamma -PAK ACTIVATION. J. Biol. Chem. 275: 16933-16940 [Abstract] [Full Text]  
  • Peron, P., Rahmani, M., Zagar, Y., Durand-Schneider, A.-M., Lardeux, B., Bernuau, D. (2001). Potentiation of Smad Transactivation by Jun Proteins during a Combined Treatment with Epidermal Growth Factor and Transforming Growth Factor-beta in Rat Hepatocytes. ROLE OF PHOSPHATIDYLINOSITOL 3-KINASE-INDUCED AP-1 ACTIVATION. J. Biol. Chem. 276: 10524-10531 [Abstract] [Full Text]  
  • Murasawa, S., Matsubara, H., Mori, Y., Masaki, H., Tsutsumi, Y., Shibasaki, Y., Kitabayashi, I., Tanaka, Y., Fujiyama, S., Koyama, Y., Fujiyama, A., Iba, S., Iwasaka, T. (2000). Angiotensin II Initiates Tyrosine Kinase Pyk2-dependent Signalings Leading to Activation of Rac1-mediated c-Jun NH2-terminal Kinase. J. Biol. Chem. 275: 26856-26863 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»