This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goodier, J. L.
Right arrow Articles by Maraia, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goodier, J. L.
Right arrow Articles by Maraia, R. J.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 10 1997, 5823-5832, Vol 17, No. 10
Copyright © 1997, American Society for Microbiology

A carboxy-terminal basic region controls RNA polymerase III transcription factor activity of human La protein

JL Goodier, H Fan and RJ Maraia
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.

Human La protein has been shown to serve as a transcription factor for RNA polymerase III (pol III) by facilitating transcription termination and recycling of transcription complexes. In addition, La binds to the 3' oligo(U) ends common to all nascent pol III transcripts, and in the case of B1-Alu RNA, protects it from 3'-end processing (R. J. Maraia, D. J. Kenan, and J. D. Keene, Mol. Cell. Biol. 14:2147-2158, 1994). Others have previously dissected the La protein into an N-terminal domain that binds RNA and a C-terminal domain that does not. Here, deletion and substitution mutants of La were examined for general RNA binding, RNA 3'-end protection, and transcription factor activity. Although some La mutants altered in a C-terminal basic region bind RNA in mobility shift assays, they are defective in RNA 3'-end protection and do not support transcription, while one C-terminal substitution mutant is defective only in transcription. Moreover, a C-terminal fragment lacking RNA binding activity appears able to support low levels of transcription by pol III. While efficient multiround transcription is supported only by mutants that bind RNA and contain a C-terminal basic region. These analyses indicate that RNA binding contributes to but is not sufficient for La transcription factor activity and that the C-terminal domain plays a role in transcription that is distinguishable from simple RNA binding. The transcription factor activity of La can be reversibly inhibited by RNA, suggesting the potential for feedback inhibition of pol III transcription.


This article has been cited by other articles:

  • Vashist, S., Anantpadma, M., Sharma, H., Vrati, S. (2009). La protein binds the predicted loop structures in the 3' non-coding region of Japanese encephalitis virus genome: role in virus replication. J. Gen. Virol. 90: 1343-1352 [Abstract] [Full Text]  
  • Mondal, T., Ray, U., Manna, A. K., Gupta, R., Roy, S., Das, S. (2008). Structural Determinant of Human La Protein Critical for Internal Initiation of Translation of Hepatitis C Virus RNA. J. Virol. 82: 11927-11938 [Abstract] [Full Text]  
  • Bitko, V., Musiyenko, A., Bayfield, M. A., Maraia, R. J., Barik, S. (2008). Cellular La Protein Shields Nonsegmented Negative-Strand RNA Viral Leader RNA from RIG-I and Enhances Virus Growth by Diverse Mechanisms. J. Virol. 82: 7977-7987 [Abstract] [Full Text]  
  • WOLIN, S.L., WURTMANN, E.J. (2006). Molecular Chaperones and Quality Control in Noncoding RNA Biogenesis. Cold Spring Harb Symp Quant Biol 71: 505-511 [Abstract]  
  • Fairley, J. A., Kantidakis, T., Kenneth, N. S., Intine, R. V., Maraia, R. J., White, R. J. (2005). Human La is found at RNA polymerase III-transcribed genes in vivo. Proc. Natl. Acad. Sci. USA 102: 18350-18355 [Abstract] [Full Text]  
  • Brenet, F., Dussault, N., Borch, J., Ferracci, G., Delfino, C., Roepstorff, P., Miquelis, R., Ouafik, L. (2005). Mammalian Peptidylglycine {alpha}-Amidating Monooxygenase mRNA Expression Can Be Modulated by the La Autoantigen. Mol. Cell. Biol. 25: 7505-7521 [Abstract] [Full Text]  
  • Pudi, R., Ramamurthy, S. S., Das, S. (2005). A Peptide Derived from RNA Recognition Motif 2 of Human La Protein Binds to Hepatitis C Virus Internal Ribosome Entry Site, Prevents Ribosomal Assembly, and Inhibits Internal Initiation of Translation. J. Virol. 79: 9842-9853 [Abstract] [Full Text]  
  • Intine, R. V., Dundr, M., Vassilev, A., Schwartz, E., Zhao, Y., Zhao, Y., DePamphilis, M. L., Maraia, R. J. (2004). Nonphosphorylated Human La Antigen Interacts with Nucleolin at Nucleolar Sites Involved in rRNA Biogenesis. Mol. Cell. Biol. 24: 10894-10904 [Abstract] [Full Text]  
  • Horke, S., Reumann, K., Schulze, C., Grosse, F., Heise, T. (2004). The La Motif and the RNA Recognition Motifs of Human La Autoantigen Contribute Individually to RNA Recognition and Subcellular Localization. J. Biol. Chem. 279: 50302-50309 [Abstract] [Full Text]  
  • Izumi, R. E., Das, S., Barat, B., Raychaudhuri, S., Dasgupta, A. (2004). A Peptide from Autoantigen La Blocks Poliovirus and Hepatitis C Virus Cap-Independent Translation and Reveals a Single Tyrosine Critical for La RNA Binding and Translation Stimulation. J. Virol. 78: 3763-3776 [Abstract] [Full Text]  
  • Pudi, R., Abhiman, S., Srinivasan, N., Das, S. (2003). Hepatitis C Virus Internal Ribosome Entry Site-mediated Translation Is Stimulated by Specific Interaction of Independent Regions of Human La Autoantigen. J. Biol. Chem. 278: 12231-12240 [Abstract] [Full Text]  
  • Horke, S., Reumann, K., Rang, A., Heise, T. (2002). Molecular Characterization of the Human La Protein{middle dot}Hepatitis B Virus RNA.B Interaction in Vitro. J. Biol. Chem. 277: 34949-34958 [Abstract] [Full Text]  
  • Cloutier, T. E., Librizzi, M. D., Mollah, A. K. M. M., Brenowitz, M., Willis, I. M. (2001). Kinetic trapping of DNA by transcription factor IIIB. Proc. Natl. Acad. Sci. USA 10.1073/pnas.161292298v1 [Abstract] [Full Text]  
  • Huang, Y., Maraia, R. J. (2001). Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human. Nucleic Acids Res 29: 2675-2690 [Abstract] [Full Text]  
  • Maraia, R. J., Intine, R. V. A. (2001). Recognition of Nascent RNA by the Human La Antigen: Conserved and Divergent Features of Structure and Function. Mol. Cell. Biol. 21: 367-379 [Full Text]  
  • Tan, Q., Li, X., Sadhale, P. P., Miyao, T., Woychik, N. A. (2000). Multiple Mechanisms of Suppression Circumvent Transcription Defects in an RNA Polymerase Mutant. Mol. Cell. Biol. 20: 8124-8133 [Abstract] [Full Text]  
  • Weser, S., Bachmann, M., Seifart, K. H., Mei{beta}ner, W. (2000). Transcription efficiency of human polymerase III genes in vitro does not depend on the RNP-forming autoantigen La. Nucleic Acids Res 28: 3935-3942 [Abstract] [Full Text]  
  • Crosio, C., Boyl, P. P., Loreni, F., Pierandrei-Amaldi, P., Amaldi, F. (2000). La protein has a positive effect on the translation of TOP mRNAs in vivo. Nucleic Acids Res 28: 2927-2934 [Abstract] [Full Text]  
  • Sobel, S. G., Wolin, S. L. (1999). Two Yeast La Motif-containing Proteins Are RNA-binding Proteins that Associate with Polyribosomes. Mol. Biol. Cell 10: 3849-3862 [Abstract] [Full Text]  
  • Shiroki, K., Isoyama, T., Kuge, S., Ishii, T., Ohmi, S., Hata, S., Suzuki, K., Takasaki, Y., Nomoto, A. (1999). Intracellular Redistribution of Truncated La Protein Produced by Poliovirus 3Cpro-Mediated Cleavage. J. Virol. 73: 2193-2200 [Abstract] [Full Text]  
  • Goodier, J. L., Maraia, R. J. (1998). Terminator-specific Recycling of a B1-Alu Transcription Complex by RNA Polymerase III Is Mediated by the RNA Terminus-binding Protein La. J. Biol. Chem. 273: 26110-26116 [Abstract] [Full Text]  
  • Fan, H., Goodier, J. L., Chamberlain, J. R., Engelke, D. R., Maraia, R. J. (1998). 5' Processing of tRNA Precursors Can Be Modulated by the Human La Antigen Phosphoprotein. Mol. Cell. Biol. 18: 3201-3211 [Abstract] [Full Text]  
  • Ali, N., Pruijn, G. J. M., Kenan, D. J., Keene, J. D., Siddiqui, A. (2000). Human La Antigen Is Required for the Hepatitis C Virus Internal Ribosome Entry Site-mediated Translation. J. Biol. Chem. 275: 27531-27540 [Abstract] [Full Text]  
  • Chong, S. S., Hu, P., Hernandez, N. (2001). Reconstitution of Transcription from the Human U6 Small Nuclear RNA Promoter with Eight Recombinant Polypeptides and a Partially Purified RNA Polymerase III Complex. J. Biol. Chem. 276: 20727-20734 [Abstract] [Full Text]  
  • Ohndorf, U.-M., Steegborn, C., Knijff, R., Sondermann, P. (2001). Contributions of the Individual Domains in Human La Protein to Its RNA 3'-End Binding Activity. J. Biol. Chem. 276: 27188-27196 [Abstract] [Full Text]  
  • Cloutier, T. E., Librizzi, M. D., Mollah, A. K. M. M., Brenowitz, M., Willis, I. M. (2001). Kinetic trapping of DNA by transcription factor IIIB. Proc. Natl. Acad. Sci. USA 98: 9581-9586 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»