This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pise-Masison, C. A.
Right arrow Articles by Brady, J. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pise-Masison, C. A.
Right arrow Articles by Brady, J. N.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 03 1997, 1236-1243, Vol 17, No. 3
Copyright © 1997, American Society for Microbiology

Physical and functional interaction between the human T-cell lymphotropic virus type 1 Tax1 protein and the CCAAT binding protein NF- Y

CA Pise-Masison, J Dittmer, KE Clemens and JN Brady
Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-5055, USA.

Tax1, a potent activator of human T-cell lymphotropic virus type 1 (HTLV-1) transcription, has been shown to modulate expression of many cellular genes. Tax1 does not bind DNA directly but regulates transcription through protein-protein interactions with sequence- specific transcription factors. Using the yeast two-hybrid system to screen for proteins which interact with Tax1, we isolated the B subunit of the CCAAT binding protein NF-Y from a HeLa cDNA library. The interaction of Tax1 with NF-YB was specific in that NF-YB did not interact with a variety of other transcription factors, including human immunodeficiency virus Tat, human papillomavirus E6, and Bicoid, or with the M7 (amino acids 29CP-AS) Tax1 mutant. However, NF-YB did interact with the C-terminal Tax1 mutants M22 (130TL-AS) and M47 (319LL- RS). We also show that in vitro-translated NF-YB specifically bound to a glutathione S-transferase-Tax1 fusion protein. Further, Tax1 coimmunoprecipitated with NF-Y from nuclear extracts of HTLV-1- transformed cells, providing evidence for in vivo interaction of Tax1 and NF-YB. We further demonstrate that Tax1 specifically activated the NF-Y-responsive DQbeta promoter, as well as a minimal promoter which contains only the Y-box element. In addition, mutation of the Y-box element alone abrogated Tax1-mediated activation. Taken together, these data indicate that Tax1 interacts with NF-Y through the B subunit and that this interaction results in activation of the major histocompatibility complex class II promoter. Through activation of this and other NF-Y driven promoters, the Tax1-NF-Y interaction may play a critical role in causing cellular transformation and HTLV-1 pathogenesis.


This article has been cited by other articles:

  • Tosi, G., Pilotti, E., Mortara, L., Barbaro, A. D. L., Casoli, C., Accolla, R. S. (2006). Inhibition of human T cell leukemia virus type 2 replication by the suppressive action of class II transactivator and nuclear factor Y. Proc. Natl. Acad. Sci. USA 103: 12861-12866 [Abstract] [Full Text]  
  • Tanaka, H., Ohshima, N., Ikenoya, M., Komori, K., Katoh, F., Hidaka, H. (2003). HMN-176, an Active Metabolite of the Synthetic Antitumor Agent HMN-214, Restores Chemosensitivity to Multidrug-Resistant Cells by Targeting the Transcription Factor NF-Y. Cancer Res. 63: 6942-6947 [Abstract] [Full Text]  
  • Lee, D. K., Kim, B.-C., Brady, J. N., Jeang, K.-T., Kim, S.-J. (2002). Human T-cell Lymphotropic Virus Type 1 Tax Inhibits Transforming Growth Factor-beta Signaling by Blocking the Association of Smad Proteins with Smad-binding Element. J. Biol. Chem. 277: 33766-33775 [Abstract] [Full Text]  
  • Pise-Masison, C. A., Radonovich, M., Mahieux, R., Chatterjee, P., Whiteford, C., Duvall, J., Guillerm, C., Gessain, A., Brady, J. N. (2002). Transcription Profile of Cells Infected with Human T-cell Leukemia Virus Type I Compared with Activated Lymphocytes. Cancer Res. 62: 3562-3571 [Abstract] [Full Text]  
  • Jiang, H., Lu, H., Schiltz, R. L., Pise-Masison, C. A., Ogryzko, V. V., Nakatani, Y., Brady, J. N. (1999). PCAF Interacts with Tax and Stimulates Tax Transactivation in a Histone Acetyltransferase-Independent Manner. Mol. Cell. Biol. 19: 8136-8145 [Abstract] [Full Text]  
  • Liu, X., Chen, X., Zachar, V., Chang, C., Ebbesen, P. (1999). Transcriptional activation of human TR3/nur77 gene expression by human T-lymphotropic virus type I Tax protein through two AP-1-like elements. J. Gen. Virol. 80: 3073-3081 [Abstract] [Full Text]  
  • Taira, T., Sawai, M., Ikeda, M., Tamai, K., Iguchi-Ariga, S. M. M., Ariga, H. (1999). Cell Cycle-dependent Switch of Up- and Down-regulation of Human hsp70 Gene Expression by Interaction between c-Myc and CBF/NF-Y. J. Biol. Chem. 274: 24270-24279 [Abstract] [Full Text]  
  • Kao, C.-Y., Tanimoto, A., Arima, N., Sasaguri, Y., Padmanabhan, R. (1999). Transactivation of the Human cdc2 Promoter by Adenovirus E1A. E1A INDUCES THE EXPRESSION AND ASSEMBLY OF A HETEROMERIC COMPLEX CONSISTING OF THE CCAAT BOX BINDING FACTOR, CBF/NF-Y, AND A 110-kDa DNA-BINDING PROTEIN. J. Biol. Chem. 274: 23043-23051 [Abstract] [Full Text]  
  • Blumenthal, S. G., Aichele, G., Wirth, T., Czernilofsky, A. P., Nordheim, A., Dittmer, J. (1999). Regulation of the Human Interleukin-5 Promoter by Ets Transcription Factors. ETS1 AND ETS2, BUT NOT ELF-1, COOPERATE WITH GATA3 AND HTLV-I TAX1. J. Biol. Chem. 274: 12910-12916 [Abstract] [Full Text]  
  • Tanaka, H., Ohshima, N., Hidaka, H. (1999). Isolation of cDNAs Encoding Cellular Drug-Binding Proteins Using a Novel Expression Cloning Procedure: Drug-Western. Mol. Pharmacol. 55: 356-363 [Abstract] [Full Text]  
  • Gachon, F., Peleraux, A., Thebault, S., Dick, J., Lemasson, I., Devaux, C., Mesnard, J.-M. (1998). CREB-2, a Cellular CRE-Dependent Transcription Repressor, Functions in Association with Tax as an Activator of the Human T-Cell Leukemia Virus Type 1 Promoter. J. Virol. 72: 8332-8337 [Abstract] [Full Text]  
  • Lemasson, I., Thebault, S., Sardet, C., Devaux, C., Mesnard, J.-M. (1998). Activation of E2F-mediated Transcription by Human T-cell Leukemia Virus Type I Tax Protein in a p16INK4A-negative T-cell Line. J. Biol. Chem. 273: 23598-23604 [Abstract] [Full Text]  
  • Eggers, A., Siemann, G., Blume, R., Knepel, W. (1998). Gene-specific Transcriptional Activity of the Insulin cAMP-responsive Element Is Conferred by NF-Y in Combination with cAMP Response Element-binding Protein. J. Biol. Chem. 273: 18499-18508 [Abstract] [Full Text]  
  • Yamada, K., Tanaka, T., Miyamoto, K., Noguchi, T. (2000). Sp Family Members and Nuclear Factor-Y Cooperatively Stimulate Transcription from the Rat Pyruvate Kinase M Gene Distal Promoter Region via Their Direct Interactions. J. Biol. Chem. 275: 18129-18137 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»