This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Levy-Strumpf, N.
Right arrow Articles by Kimchi, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Levy-Strumpf, N.
Right arrow Articles by Kimchi, A.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Mar 1997, 1615-1625, Vol 17, No. 3
Copyright © 1997, American Society for Microbiology

DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death

N Levy-Strumpf, LP Deiss, H Berissi and A Kimchi
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.

A functional approach to gene cloning was applied to HeLa cells in an attempt to isolate cDNA fragments which convey resistance to gamma interferon (IFN-gamma)-induced programmed cell death. One of the rescued cDNAs, described in this work, was a fragment of a novel gene, named DAP-5. Analysis of a DAP-5 full-length cDNA clone revealed that it codes for a 97-kDa protein that is highly homologous to eukaryotic translation initiation factor 4G (eIF4G, also known as p220). According to its deduced amino acid sequence, this novel protein lacks the N- terminal region of eIF4G responsible for association with the cap binding protein eIF4E. The N-terminal part of DAP-5 has 39% identity and 63% similarity to the central region of mammalian p220. Its C- terminal part is less homologous to the corresponding region of p220, suggesting that it may possess unique functional properties. The rescued DAP-5 cDNA fragment which conveyed resistance to IFN-gamma- induced cell death was expressed from the vector in the sense orientation. Intriguingly, it comprised part of the coding region which corresponds to the less conserved C-terminal part of DAP-5 and directed the synthesis of a 28-kDa miniprotein. The miniprotein exerted a dual effect on HeLa cells. Low levels of expression protected the cells from IFN-gamma-induced programmed cell death, while high levels of expression were not compatible with continuous cell growth. The relevance of DAP-5 protein to possible changes in a cell's translational machinery during programmed cell death and growth arrest is discussed.


This article has been cited by other articles:

  • Mebratu, Y. A., Dickey, B. F., Evans, C., Tesfaigzi, Y. (2008). The BH3-only protein Bik/Blk/Nbk inhibits nuclear translocation of activated ERK1/2 to mediate IFN{gamma}-induced cell death. JCB 183: 429-439 [Abstract] [Full Text]  
  • Lewis, S. M., Cerquozzi, S., Graber, T. E., Ungureanu, N. H., Andrews, M., Holcik, M. (2008). The eIF4G homolog DAP5/p97 supports the translation of select mRNAs during endoplasmic reticulum stress. Nucleic Acids Res 36: 168-178 [Abstract] [Full Text]  
  • Nousch, M., Reed, V., Bryson-Richardson, R. J., Currie, P. D., Preiss, T. (2007). The eIF4G-homolog p97 can activate translation independent of caspase cleavage. RNA 13: 374-384 [Abstract] [Full Text]  
  • Okomo-Adhiambo, M., Beattie, C., Rink, A. (2006). cDNA Microarray Analysis of Host-Pathogen Interactions in a Porcine In Vitro Model for Toxoplasma gondii Infection. Infect. Immun. 74: 4254-4265 [Abstract] [Full Text]  
  • Hundsdoerfer, P., Thoma, C., Hentze, M. W. (2005). Eukaryotic translation initiation factor 4GI and p97 promote cellular internal ribosome entry sequence-driven translation. Proc. Natl. Acad. Sci. USA 102: 13421-13426 [Abstract] [Full Text]  
  • Yanagiya, A., Jia, Q., Ohka, S., Horie, H., Nomoto, A. (2005). Blockade of the Poliovirus-Induced Cytopathic Effect in Neural Cells by Monoclonal Antibody against Poliovirus or the Human Poliovirus Receptor. J. Virol. 79: 1523-1532 [Abstract] [Full Text]  
  • Schroder, K., Hertzog, P. J., Ravasi, T., Hume, D. A. (2004). Interferon-{gamma}: an overview of signals, mechanisms and functions. J. Leukoc. Biol. 75: 163-189 [Abstract] [Full Text]  
  • Li, S., Takasu, T., Perlman, D. M., Peterson, M. S., Burrichter, D., Avdulov, S., Bitterman, P. B., Polunovsky, V. A. (2003). Translation Factor eIF4E Rescues Cells from Myc-dependent Apoptosis by Inhibiting Cytochrome c Release. J. Biol. Chem. 278: 3015-3022 [Abstract] [Full Text]  
  • Lin, Y., Khokhlatchev, A., Figeys, D., Avruch, J. (2002). Death-associated Protein 4 Binds MST1 and Augments MST1-induced Apoptosis. J. Biol. Chem. 277: 47991-48001 [Abstract] [Full Text]  
  • Henis-Korenblit, S., Shani, G., Sines, T., Marash, L., Shohat, G., Kimchi, A. (2002). The caspase-cleaved DAP5 protein supports internal ribosome entry site-mediated translation of death proteins. Proc. Natl. Acad. Sci. USA 99: 5400-5405 [Abstract] [Full Text]  
  • Bradley, C. A., Padovan, J. C., Thompson, T. L., Benoit, C. A., Chait, B. T., Rhoads, R. E. (2002). Mass Spectrometric Analysis of the N Terminus of Translational Initiation Factor eIF4G-1 Reveals Novel Isoforms. J. Biol. Chem. 277: 12559-12571 [Abstract] [Full Text]  
  • Mendell, J. T., Medghalchi, S. M., Lake, R. G., Noensie, E. N., Dietz, H. C. (2000). Novel Upf2p Orthologues Suggest a Functional Link between Translation Initiation and Nonsense Surveillance Complexes. Mol. Cell. Biol. 20: 8944-8957 [Abstract] [Full Text]  
  • Aravind, L., Koonin, E. V. (2000). Eukaryote-specific Domains in Translation Initiation Factors: Implications for Translation Regulation and Evolution of the Translation System. Genome Res 10: 1172-1184 [Abstract] [Full Text]  
  • Morino, S., Imataka, H., Svitkin, Y. V., Pestova, T. V., Sonenberg, N. (2000). Eukaryotic Translation Initiation Factor 4E (eIF4E) Binding Site and the Middle One-Third of eIF4GI Constitute the Core Domain for Cap-Dependent Translation, and the C-Terminal One-Third Functions as a Modulatory Region. Mol. Cell. Biol. 20: 468-477 [Abstract] [Full Text]  
  • Henis-Korenblit, S., Strumpf, N. L., Goldstaub, D., Kimchi, A. (2000). A Novel Form of DAP5 Protein Accumulates in Apoptotic Cells as a Result of Caspase Cleavage and Internal Ribosome Entry Site-Mediated Translation. Mol. Cell. Biol. 20: 496-506 [Abstract] [Full Text]  
  • Johnson, K. L., Ball, L. A. (1999). Induction and Maintenance of Autonomous Flock House Virus RNA1 Replication. J. Virol. 73: 7933-7942 [Abstract] [Full Text]  
  • Gudkov, A. V., Roninson, I. B., Brown;, R., Kimchi, A., Cohen, O., Kissil, J., Raveh, T., Inbal, B., Levy-Strumpf, N., Berissi, H., Deiss;, L. (1999). Functional Approaches to Gene Isolation in Mammalian Cells. Science 285: 299a-299 [Full Text]  
  • Tarrant, T. K., Silver, P. B., Wahlsten, J. L., Rizzo, L. V., Chan, C.-C., Wiggert, B., Caspi, R. R. (1999). Interleukin 12 Protects from a T Helper Type 1-mediated Autoimmune Disease, Experimental Autoimmune Uveitis, through a Mechanism Involving Interferon {gamma}, Nitric Oxide, and Apoptosis. JEM 189: 219-230 [Abstract] [Full Text]  
  • Gradi, A., Imataka, H., Svitkin, Y. V., Rom, E., Raught, B., Morino, S., Sonenberg, N. (1998). A Novel Functional Human Eukaryotic Translation Initiation Factor 4G. Mol. Cell. Biol. 18: 334-342 [Abstract] [Full Text]  
  • Henis-Korenblit, S., Shani, G., Sines, T., Marash, L., Shohat, G., Kimchi, A. (2002). The caspase-cleaved DAP5 protein supports internal ribosome entry site-mediated translation of death proteins. Proc. Natl. Acad. Sci. USA 99: 5400-5405 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»