This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Greene, C. N.
Right arrow Articles by Jinks-Robertson, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Greene, C. N.
Right arrow Articles by Jinks-Robertson, S.

 Previous Article  |  Next Article 

Mol. Cell. Biol., May 1997, 2844-2850, Vol 17, No. 5
Copyright © 1997, American Society for Microbiology

Frameshift intermediates in homopolymer runs are removed efficiently by yeast mismatch repair proteins

CN Greene and S Jinks-Robertson
Graduate Program in Genetics and Molecular Biology, Emory University, Atlanta, Georgia 30322, USA.

A change in the number of base pairs within a coding sequence can result in a frameshift mutation, which almost invariably eliminates the function of the encoded protein. A frameshift reversion assay with Saccharomyces cerevisiae that can be used to examine the types of insertions and deletions that are generated during DNA replication, as well as the editing functions that remove such replication errors, has been developed. Reversion spectra have been obtained in a wild-type strain and in strains defective for defined components of the postreplicative mismatch repair system (msh2, msh3, msh6, msh3 msh6, pms1, and mih1 mutants). Comparison of the spectra reveals that yeast mismatch repair proteins preferentially remove frameshift intermediates that arise in homopolymer tracts and indicates that some of the proteins have distinct substrate or context specificities.


This article has been cited by other articles:

  • Enserink, J. M., Hombauer, H., Huang, M.-E., Kolodner, R. D. (2009). Cdc28/Cdk1 positively and negatively affects genome stability in S. cerevisiae. JCB 185: 423-437 [Abstract] [Full Text]  
  • Nicholson, A., Fabbri, R. M., Reeves, J. W., Crouse, G. F. (2006). The Effects of Mismatch Repair and RAD1 Genes on Interchromosomal Crossover Recombination in Saccharomyces cerevisiae. Genetics 173: 647-659 [Abstract] [Full Text]  
  • Abdulovic, A. L., Jinks-Robertson, S. (2006). The in Vivo Characterization of Translesion Synthesis Across UV-Induced Lesions in Saccharomyces cerevisiae: Insights Into Pol{zeta}- and Pol{eta}-Dependent Frameshift Mutagenesis. Genetics 172: 1487-1498 [Abstract] [Full Text]  
  • Sterling, C. H., Sweasy, J. B. (2006). DNA Polymerase 4 of Saccharomyces cerevisiae Is Important for Accurate Repair of Methyl-Methanesulfonate-Induced DNA Damage. Genetics 172: 89-98 [Abstract] [Full Text]  
  • Lippert, M. J., Freedman, J. A., Barber, M. A., Jinks-Robertson, S. (2004). Identification of a Distinctive Mutation Spectrum Associated with High Levels of Transcription in Yeast. Mol. Cell. Biol. 24: 4801-4809 [Abstract] [Full Text]  
  • Evert, B. A., Salmon, T. B., Song, B., Jingjing, L., Siede, W., Doetsch, P. W. (2004). Spontaneous DNA Damage in Saccharomyces cerevisiae Elicits Phenotypic Properties Similar to Cancer Cells. J. Biol. Chem. 279: 22585-22594 [Abstract] [Full Text]  
  • Morey, N. J., Doetsch, P. W., Jinks-Robertson, S. (2003). Delineating the Requirements for Spontaneous DNA Damage Resistance Pathways in Genome Maintenance and Viability in Saccharomyces cerevisiae. Genetics 164: 443-455 [Abstract] [Full Text]  
  • Gragg, H., Harfe, B. D., Jinks-Robertson, S. (2002). Base Composition of Mononucleotide Runs Affects DNA Polymerase Slippage and Removal of Frameshift Intermediates by Mismatch Repair in Saccharomyces cerevisiae. Mol. Cell. Biol. 22: 8756-8762 [Abstract] [Full Text]  
  • Lau, P. J., Flores-Rozas, H., Kolodner, R. D. (2002). Isolation and Characterization of New Proliferating Cell Nuclear Antigen (POL30) Mutator Mutants That Are Defective in DNA Mismatch Repair. Mol. Cell. Biol. 22: 6669-6680 [Abstract] [Full Text]  
  • Tijsterman, M., Pothof, J., Plasterk, R. H. A. (2002). Frequent Germline Mutations and Somatic Repeat Instability in DNA Mismatch-Repair-Deficient Caenorhabditis elegans. Genetics 161: 651-660 [Abstract] [Full Text]  
  • Huang, M.-E., Rio, A.-G., Galibert, M.-D., Galibert, F. (2002). Pol32, a Subunit of Saccharomyces cerevisiae DNA Polymerase {delta}, Suppresses Genomic Deletions and Is Involved in the Mutagenic Bypass Pathway. Genetics 160: 1409-1422 [Abstract] [Full Text]  
  • Sia, E. A., Dominska, M., Stefanovic, L., Petes, T. D. (2001). Isolation and Characterization of Point Mutations in Mismatch Repair Genes That Destabilize Microsatellites in Yeast. Mol. Cell. Biol. 21: 8157-8167 [Abstract] [Full Text]  
  • Greene, C. N., Jinks-Robertson, S. (2001). Spontaneous Frameshift Mutations in Saccharomyces cerevisiae: Accumulation During DNA Replication and Removal by Proofreading and Mismatch Repair Activities. Genetics 159: 65-75 [Abstract] [Full Text]  
  • Tornier, C., Bessone, S., Varlet, I., Rudolph, C., Darmon, M., Fleck, O. (2001). Requirement for Msh6, but Not for Swi4 (Msh3), in Msh2-Dependent Repair of Base-Base Mismatches and Mononucleotide Loops in Schizosaccharomyces pombe. Genetics 158: 65-75 [Abstract] [Full Text]  
  • Mansour, A. A., Tornier, C., Lehmann, E., Darmon, M., Fleck, O. (2001). Control of GT Repeat Stability in Schizosaccharomyces pombe by Mismatch Repair Factors. Genetics 158: 77-85 [Abstract] [Full Text]  
  • Harfe, B. D., Jinks-Robertson, S. (2000). Sequence Composition and Context Effects on the Generation and Repair of Frameshift Intermediates in Mononucleotide Runs in Saccharomyces cerevisiae. Genetics 156: 571-578 [Abstract] [Full Text]  
  • Chen, J. Z., Qiu, J., Shen, B., Holmquist, G. P. (2000). Mutational spectrum analysis of RNase H(35) deficient Saccharomyces cerevisiae using fluorescence-based directed termination PCR. Nucleic Acids Res 28: 3649-3656 [Abstract] [Full Text]  
  • Bachtrog, D., Agis, M., Imhof, M., Schlotterer, C. (2000). Microsatellite Variability Differs Between Dinucleotide Repeat Motifs--Evidence from Drosophila melanogaster. Mol Biol Evol 17: 1277-1285 [Abstract] [Full Text]  
  • WHEELER, J M D, BODMER, W F, MORTENSEN, N J M. (2000). DNA mismatch repair genes and colorectal cancer. Gut 47: 148-153 [Full Text]  
  • Sokolsky, T., Alani, E. (2000). EXO1 and MSH6 Are High-Copy Suppressors of Conditional Mutations in the MSH2 Mismatch Repair Gene of Saccharomyces cerevisiae. Genetics 155: 589-599 [Abstract] [Full Text]  
  • Morey, N. J., Greene, C. N., Jinks-Robertson, S. (2000). Genetic Analysis of Transcription-Associated Mutation in Saccharomyces cerevisiae. Genetics 154: 109-120 [Abstract] [Full Text]  
  • Nicholson, A., Hendrix, M., Jinks-Robertson, S., Crouse, G. F. (2000). Regulation of Mitotic Homeologous Recombination in Yeast: Functions of Mismatch Repair and Nucleotide Excision Repair Genes. Genetics 154: 133-146 [Abstract] [Full Text]  
  • Chen, C., Merrill, B. J., Lau, P. J., Holm, C., Kolodner, R. D. (1999). Saccharomyces cerevisiae pol30 (Proliferating Cell Nuclear Antigen) Mutations Impair Replication Fidelity and Mismatch Repair. Mol. Cell. Biol. 19: 7801-7815 [Abstract] [Full Text]  
  • Marsischky, G. T., Kolodner, R. D. (1999). Biochemical Characterization of the Interaction between the Saccharomyces cerevisiae MSH2-MSH6 Complex and Mispaired Bases in DNA. J. Biol. Chem. 274: 26668-26682 [Abstract] [Full Text]  
  • Harfe, B. D., Jinks-Robertson, S. (1999). Removal of Frameshift Intermediates by Mismatch Repair Proteins in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 4766-4773 [Abstract] [Full Text]  
  • Tobi, S. E., Levy, D. D., Seidman1, M. M., Kraemer1, K. H. (1999). Sequence-dependent mutations in a shuttle vector plasmid replicated in a mismatch repair deficient human cell line. Carcinogenesis 20: 1293-1301 [Abstract] [Full Text]  
  • Mirabelli-Primdahl, L., Gryfe, R., Kim, H., Millar, A., Luceri, C., Dale, D., Holowaty, E., Bapat, B., Gallinger, S., Redston, M. (1999). {beta}-Catenin Mutations Are Specific for Colorectal Carcinomas with Microsatellite Instability but Occur in Endometrial Carcinomas Irrespective of Mutator Pathway. Cancer Res. 59: 3346-3351 [Abstract] [Full Text]  
  • Schweitzer, J. K., Livingston, D. M. (1999). The Effect of DNA Replication Mutations on CAG Tract Stability in Yeast. Genetics 152: 953-963 [Abstract] [Full Text]  
  • Swanson, R. L., Morey, N. J., Doetsch, P. W., Jinks-Robertson, S. (1999). Overlapping Specificities of Base Excision Repair, Nucleotide Excision Repair, Recombination, and Translesion Synthesis Pathways for DNA Base Damage in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 2929-2935 [Abstract] [Full Text]  
  • Drotschmann, K., Clark, A. B., Tran, H. T., Resnick, M. A., Gordenin, D. A., Kunkel, T. A. (1999). Mutator phenotypes of yeast strains heterozygous for mutations in the MSH2 gene. Proc. Natl. Acad. Sci. USA 96: 2970-2975 [Abstract] [Full Text]  
  • Tran, H. T., Gordenin, D. A., Resnick, M. A. (1999). The 3'right-arrow5' Exonucleases of DNA Polymerases delta  and varepsilon  and the 5'right-arrow3' Exonuclease Exo1 Have Major Roles in Postreplication Mutation Avoidance in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 2000-2007 [Abstract] [Full Text]  
  • Xie, Y., Counter, C., Alani, E. (1999). Characterization of the Repeat-Tract Instability and Mutator Phenotypes Conferred by a Tn3 Insertion in RFC1, the Large Subunit of the Yeast Clamp Loader. Genetics 151: 499-509 [Abstract] [Full Text]  
  • Flores-Rozas, H., Kolodner, R. D. (1998). The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations. Proc. Natl. Acad. Sci. USA 95: 12404-12409 [Abstract] [Full Text]  
  • Glaab, W. E., Risinger, J. I., Umar, A., Kunkel, T. A., Barrett, J. C., Tindall, K. R. (1998). Characterization of Distinct Human Endometrial Carcinoma Cell Lines Deficient in Mismatch Repair That Originated from a Single Tumor. J. Biol. Chem. 273: 26662-26669 [Abstract] [Full Text]  
  • Genschel, J., Littman, S. J., Drummond, J. T., Modrich, P. (1998). Isolation of MutSbeta from Human Cells and Comparison of the Mismatch Repair Specificities of MutSbeta and MutSalpha. J. Biol. Chem. 273: 19895-19901 [Abstract] [Full Text]  
  • DeMarini, D. M., Shelton, M. L., Abu-Shakra, A., Szakmary, A., Levine, J. G. (1998). Spectra of Spontaneous Frameshift Mutations at the hisD3052 Allele of Salmonella typhimurium in Four DNA Repair Backgrounds. Genetics 149: 17-36 [Abstract] [Full Text]  
  • Umar, A., Risinger, J. I., Glaab, W. E., Tindall, K. R., Barrett, J. C., Kunkel, T. A. (1998). Functional Overlap in Mismatch Repair by Human MSH3 and MSH6. Genetics 148: 1637-1646 [Abstract] [Full Text]  
  • Hohl, M., Christensen, O., Kunz, C., Naegeli, H., Fleck, O. (2001). Binding and Repair of Mismatched DNA Mediated by Rhp14, the Fission Yeast Homologue of Human XPA. J. Biol. Chem. 276: 30766-30772 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»