This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pessler, F.
Right arrow Articles by Hernandez, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pessler, F.
Right arrow Articles by Hernandez, N.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 07 1997, 3786-3798, Vol 17, No. 7
Copyright © 1997, American Society for Microbiology

Purification and characterization of FBI-1, a cellular factor that binds to the human immunodeficiency virus type 1 inducer of short transcripts

F Pessler, PS Pendergrast and N Hernandez
Cold Spring Harbor Laboratory, New York 11724, USA.

The human immunodeficiency virus (HIV-1) promoter directs the synthesis of two classes of RNA molecules, short transcripts and full-length transcripts. The synthesis of short transcripts depends on a bipartite DNA element, the inducer of short transcripts (IST), located in large part downstream of the HIV-1 start site of transcription. IST does not require any viral product for function and is thought to direct the assembly of transcription complexes that are incapable of efficient elongation. Nothing is known, however, about the biochemical mechanisms that mediate IST function. Here, we report the identification and purification of a factor that binds specifically to the IST. This factor, FBI-1, recognizes a large bipartite binding site that coincides with the bipartite IST element. It is constituted at least in part by an 86-kDa polypeptide that can be specifically cross-linked to IST. FBI- 1 also binds to promoter and attenuation regions of a number of cellular and viral transcription units that are regulated by a transcription elongation block. This observation, together with the observation that the binding of FBI-1 to IST mutants correlates with the ability of these mutants to direct IST function, suggests that FBI- 1 may be involved in the establishment of abortive transcription complexes.


This article has been cited by other articles:

  • Choi, W.-I., Jeon, B.-N., Yun, C.-O., Kim, P.-H., Kim, S.-E., Choi, K.-Y., Kim, S. H., Hur, M.-W. (2009). Proto-oncogene FBI-1 Represses Transcription of p21CIP1 by Inhibition of Transcription Activation by p53 and Sp1. J. Biol. Chem. 284: 12633-12644 [Abstract] [Full Text]  
  • Choi, W.-I., Jeon, B.-N., Park, H., Yoo, J.-Y., Kim, Y.-S., Koh, D.-I., Kim, M.-H., Kim, Y.-R., Lee, C.-E., Kim, K.-S., Osborne, T. F., Hur, M.-W. (2008). Proto-oncogene FBI-1 (Pokemon) and SREBP-1 Synergistically Activate Transcription of Fatty-acid Synthase Gene (FASN). J. Biol. Chem. 283: 29341-29354 [Abstract] [Full Text]  
  • Maeda, T., Merghoub, T., Hobbs, R. M., Dong, L., Maeda, M., Zakrzewski, J., van den Brink, M. R.M., Zelent, A., Shigematsu, H., Akashi, K., Teruya-Feldstein, J., Cattoretti, G., Pandolfi, P. P. (2007). Regulation of B Versus T Lymphoid Lineage Fate Decision by the Proto-Oncogene LRF. Science 316: 860-866 [Abstract] [Full Text]  
  • Costoya, J. A. (2007). Functional analysis of the role of POK transcriptional repressors. Brief Funct Genomic Proteomic 0: elm002v1-11 [Abstract] [Full Text]  
  • Maeda, T., Hobbs, R. M., Pandolfi, P. P. (2005). The Transcription Factor Pokemon: A New Key Player in Cancer Pathogenesis. Cancer Res. 65: 8575-8578 [Abstract] [Full Text]  
  • Lee, D.-K., Kang, J.-E., Park, H.-J., Kim, M.-H., Yim, T.-H., Kim, J.-M., Heo, M.-K., Kim, K.-Y., Kwon, H. J., Hur, M.-W. (2005). FBI-1 Enhances Transcription of the Nuclear Factor-{kappa}B (NF-{kappa}B)-responsive E-selectin Gene by Nuclear Localization of the p65 Subunit of NF-{kappa}B. J. Biol. Chem. 280: 27783-27791 [Abstract] [Full Text]  
  • Liu, C.-j., Prazak, L., Fajardo, M., Yu, S., Tyagi, N., Di Cesare, P. E. (2004). Leukemia/Lymphoma-related Factor, a POZ Domain-containing Transcriptional Repressor, Interacts with Histone Deacetylase-1 and Inhibits Cartilage Oligomeric Matrix Protein Gene Expression and Chondrogenesis. J. Biol. Chem. 279: 47081-47091 [Abstract] [Full Text]  
  • Laudes, M., Christodoulides, C., Sewter, C., Rochford, J. J., Considine, R. V., Sethi, J. K., Vidal-Puig, A., O'Rahilly, S. (2004). Role of the POZ Zinc Finger Transcription Factor FBI-1 in Human and Murine Adipogenesis. J. Biol. Chem. 279: 11711-11718 [Abstract] [Full Text]  
  • Pessler, F., Hernandez, N. (2003). Flexible DNA Binding of the BTB/POZ-domain Protein FBI-1. J. Biol. Chem. 278: 29327-29335 [Abstract] [Full Text]  
  • Astier, A. L., Xu, R., Svoboda, M., Hinds, E., Munoz, O., de Beaumont, R., Crean, C. D., Gabig, T., Freedman, A. S. (2003). Temporal gene expression profile of human precursor B leukemia cells induced by adhesion receptor: identification of pathways regulating B-cell survival. Blood 101: 1118-1127 [Abstract] [Full Text]  
  • Pendergrast, P. S., Wang, C., Hernandez, N., Huang, S. (2002). FBI-1 Can Stimulate HIV-1 Tat Activity and Is Targeted to a Novel Subnuclear Domain that Includes the Tat-P-TEFb---containing Nuclear Speckles. Mol. Biol. Cell 13: 915-929 [Abstract] [Full Text]  
  • Pereira, L. A., Bentley, K., Peeters, A., Churchill, M. J, Deacon, N. J. (2000). SURVEY AND SUMMARY A compilation of cellular transcription factor interactions with the HIV-1 LTR promoter. Nucleic Acids Res 28: 663-668 [Abstract] [Full Text]  
  • Pessler, F., Hernandez, N. (1998). The HIV-1 Inducer of Short Transcripts Activates the Synthesis of 5,6-Dichloro-1-beta -D-benzimidazole-resistant Short Transcripts in Vitro. J. Biol. Chem. 273: 5375-5384 [Abstract] [Full Text]  
  • Jones, K. A. (1997). Taking a new TAK on Tat transactivation. Genes Dev. 11: 2593-2599 [Full Text]  
  • Marshall, N. F., Dahmus, M. E. (2000). C-terminal Domain Phosphatase Sensitivity of RNA Polymerase II in Early Elongation Complexes on the HIV-1 and Adenovirus 2 Major Late Templates. J. Biol. Chem. 275: 32430-32437 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»