This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kiledjian, M.
Right arrow Articles by Novick, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kiledjian, M.
Right arrow Articles by Novick, K.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Aug 1997, 4870-4876, Vol 17, No. 8
Copyright © 1997, American Society for Microbiology

Identification of AUF1 (heterogeneous nuclear ribonucleoprotein D) as a component of the alpha-globin mRNA stability complex

M Kiledjian, CT DeMaria, G Brewer and K Novick
Department of Cell, Developmental and Neurobiology, Rutgers University, Piscataway, New Jersey 08855, USA. kiledjia@biology.rutgers.edu

mRNA turnover is an important regulatory component of gene expression and is significantly influenced by ribonucleoprotein (RNP) complexes which form on the mRNA. Studies of human alpha-globin mRNA stability have identified a specific RNP complex (alpha-complex) which forms on the 3' untranslated region (3'UTR) of the mRNA and appears to regulate the erythrocyte-specific accumulation of alpha-globin mRNA. One of the protein activities in this multiprotein complex is a poly(C)-binding activity which consists of two proteins, alphaCP1 and alphaCP2. Neither of these proteins, individually or as a pair, can bind the alpha-globin 3'UTR unless they are complexed with the remaining non-poly(C) binding proteins of the alpha-complex. With the yeast two-hybrid screen, a second alpha-complex protein was identified. This protein is a member of the previously identified A+U-rich (ARE) binding/degradation factor (AUF1) family of proteins, which are also known as the heterogeneous nuclear RNP (hnRNP) D proteins. We refer to these proteins as AUF1/hnRNP-D. Thus, a protein implicated in ARE-mediated mRNA decay is also an integral component of the mRNA stabilizing alpha-complex. The interaction of AUF1/hnRNP-D is more efficient with alphaCP1 relative to alphaCP2 both in vitro and in vivo, suggesting that the alpha-complex might be dynamic rather than a fixed complex. AUF1/hnRNP-D could, therefore, be a general mRNA turnover factor involved in both stabilization and decay of mRNA.


This article has been cited by other articles:

  • Nechama, M., Ben-Dov, I. Z., Silver, J., Naveh-Many, T. (2009). Regulation of PTH mRNA stability by the calcimimetic R568 and the phosphorus binder lanthanum carbonate in CKD. Am. J. Physiol. Renal Physiol. 296: F795-F800 [Abstract] [Full Text]  
  • Palanisamy, V., Park, N.J., Wang, J., Wong, D.T. (2008). AUF1 and HuR Proteins Stabilize Interleukin-8 mRNA in Human Saliva. JDR 87: 772-776 [Abstract] [Full Text]  
  • Moulton, V. R., Kyttaris, V. C., Juang, Y.-T., Chowdhury, B., Tsokos, G. C. (2008). The RNA-stabilizing Protein HuR Regulates the Expression of {zeta} Chain of the Human T Cell Receptor-associated CD3 Complex. J. Biol. Chem. 283: 20037-20044 [Abstract] [Full Text]  
  • Ing, N. H., Massuto, D. A., Jaeger, L. A. (2008). Estradiol Up-regulates AUF1p45 Binding to Stabilizing Regions within the 3'-Untranslated Region of Estrogen Receptor {alpha} mRNA. J. Biol. Chem. 283: 1764-1772 [Abstract] [Full Text]  
  • Wang, Z., Pandey, A., Hart, G. W. (2007). Dynamic Interplay between O-Linked N-Acetylglucosaminylation and Glycogen Synthase Kinase-3-dependent Phosphorylation. Mol. Cell. Proteomics 6: 1365-1379 [Abstract] [Full Text]  
  • Meng, Q., Rayala, S. K., Gururaj, A. E., Talukder, A. H., O'Malley, B. W., Kumar, R. (2007). Signaling-dependent and coordinated regulation of transcription, splicing, and translation resides in a single coregulator, PCBP1. Proc. Natl. Acad. Sci. USA 104: 5866-5871 [Abstract] [Full Text]  
  • Lu, J.-Y., Sadri, N., Schneider, R. J. (2006). Endotoxic shock in AUF1 knockout mice mediated by failure to degrade proinflammatory cytokine mRNAs. Genes Dev. 20: 3174-3184 [Abstract] [Full Text]  
  • Dinur, M., Kilav, R., Sela-Brown, A., Jacquemin-Sablon, H., Naveh-Many, T. (2006). In Vitro Evidence that Upstream of N-ras Participates in the Regulation of Parathyroid Hormone Messenger Ribonucleic Acid Stability. Mol. Endocrinol. 20: 1652-1660 [Abstract] [Full Text]  
  • Lu, J.-Y., Bergman, N., Sadri, N., Schneider, R. J. (2006). Assembly of AUF1 with eIF4G-poly(A) binding protein complex suggests a translation function in AU-rich mRNA decay. RNA 12: 883-893 [Abstract] [Full Text]  
  • Jiang, Y., Xu, X.-S., Russell, J. E. (2006). A Nucleolin-Binding 3' Untranslated Region Element Stabilizes {beta}-Globin mRNA In Vivo.. Mol. Cell. Biol. 26: 2419-2429 [Abstract] [Full Text]  
  • Arao, Y., Kikuchi, A., Kishida, M., Yonekura, M., Inoue, A., Yasuda, S., Wada, S., Ikeda, K., Kayama, F. (2004). Stability of A+U-Rich Element Binding Factor 1 (AUF1)-Binding Messenger Ribonucleic Acid Correlates with the Subcellular Relocalization of AUF1 in the Rat Uterus upon Estrogen Treatment. Mol. Endocrinol. 18: 2255-2267 [Abstract] [Full Text]  
  • Raineri, I., Wegmueller, D., Gross, B., Certa, U., Moroni, C. (2004). Roles of AUF1 isoforms, HuR and BRF1 in ARE-dependent mRNA turnover studied by RNA interference. Nucleic Acids Res 32: 1279-1288 [Abstract] [Full Text]  
  • HAILE, S., ESTEVEZ, A. M., CLAYTON, C. (2003). A role for the exosome in the in vivo degradation of unstable mRNAs. RNA 9: 1491-1501 [Abstract] [Full Text]  
  • Chkheidze, A. N., Liebhaber, S. A. (2003). A Novel Set of Nuclear Localization Signals Determine Distributions of the {alpha}CP RNA-Binding Proteins. Mol. Cell. Biol. 23: 8405-8415 [Abstract] [Full Text]  
  • Waggoner, S. A., Liebhaber, S. A. (2003). Identification of mRNAs Associated with {alpha}CP2-Containing RNP Complexes. Mol. Cell. Biol. 23: 7055-7067 [Abstract] [Full Text]  
  • Waggoner, S. A., Liebhaber, S. A. (2003). Regulation of {alpha}-Globin mRNA Stability. Exp. Biol. Med. 228: 387-395 [Abstract] [Full Text]  
  • Kong, J., Ji, X., Liebhaber, S. A. (2003). The KH-Domain Protein {alpha}CP Has a Direct Role in mRNA Stabilization Independent of Its Cognate Binding Site. Mol. Cell. Biol. 23: 1125-1134 [Abstract] [Full Text]  
  • Coyle, J. H., Guzik, B. W., Bor, Y.-C., Jin, L., Eisner-Smerage, L., Taylor, S. J., Rekosh, D., Hammarskjold, M.-L. (2003). Sam68 Enhances the Cytoplasmic Utilization of Intron-Containing RNA and Is Functionally Regulated by the Nuclear Kinase Sik/BRK. Mol. Cell. Biol. 23: 92-103 [Abstract] [Full Text]  
  • Pioli, P. A., Hamilton, B. J., Connolly, J. E., Brewer, G., Rigby, W. F. C. (2002). Lactate Dehydrogenase Is an AU-rich Element-binding Protein That Directly Interacts with AUF1. J. Biol. Chem. 277: 35738-35745 [Abstract] [Full Text]  
  • Silver, J., Kilav, R., Naveh-Many, T. (2002). Mechanisms of secondary hyperparathyroidism. Am. J. Physiol. Renal Physiol. 283: F367-F376 [Abstract] [Full Text]  
  • Laroia, G., Schneider, R. J. (2002). Alternate exon insertion controls selective ubiquitination and degradation of different AUF1 protein isoforms. Nucleic Acids Res 30: 3052-3058 [Abstract] [Full Text]  
  • Perez-Amador, M. A., Lidder, P., Johnson, M. A., Landgraf, J., Wisman, E., Green, P. J. (2001). New Molecular Phenotypes in the dst Mutants of Arabidopsis Revealed by DNA Microarray Analysis. Plant Cell 13: 2703-2717 [Abstract] [Full Text]  
  • Xu, N., Chen, C.-Y. A., Shyu, A.-B. (2001). Versatile Role for hnRNP D Isoforms in the Differential Regulation of Cytoplasmic mRNA Turnover. Mol. Cell. Biol. 21: 6960-6971 [Abstract] [Full Text]  
  • Yu, J., Russell, J. E. (2001). Structural and Functional Analysis of an mRNP Complex That Mediates the High Stability of Human {beta}-Globin mRNA. Mol. Cell. Biol. 21: 5879-5888 [Abstract] [Full Text]  
  • Wang, Z., Kiledjian, M. (2000). The Poly(A)-Binding Protein and an mRNA Stability Protein Jointly Regulate an Endoribonuclease Activity. Mol. Cell. Biol. 20: 6334-6341 [Abstract] [Full Text]  
  • Brooks, S. A., Rigby, W. F. C. (2000). Characterization of the mRNA ligands bound by the RNA binding protein hnRNP A2 utilizing a novel in vivo technique. Nucleic Acids Res 28: e49-e49 [Abstract] [Full Text]  
  • Sela-Brown, A., Silver, J., Brewer, G., Naveh-Many, T. (2000). Identification of AUF1 as a Parathyroid Hormone mRNA 3'-Untranslated Region-binding Protein That Determines Parathyroid Hormone mRNA Stability. J. Biol. Chem. 275: 7424-7429 [Abstract] [Full Text]  
  • Blaxall, B. C., Pellett, A. C., Wu, S. C., Pende, A., Port, J. D. (2000). Purification and Characterization of beta -Adrenergic Receptor mRNA-binding Proteins. J. Biol. Chem. 275: 4290-4297 [Abstract] [Full Text]  
  • YALCINDAG, C., SILVER, J., NAVEH-MANY, T. (1999). Mechanism of Increased Parathyroid Hormone mRNA in Experimental Uremia: Roles of Protein RNA Binding and RNA Degradation. J. Am. Soc. Nephrol. 10: 2562-2568 [Abstract] [Full Text]  
  • Loflin, P., Chen, C.-Y. A., Shyu, A.-B. (1999). Unraveling a cytoplasmic role for hnRNP D in the in vivo mRNA destabilization directed by the AU-rich element. Genes Dev. 13: 1884-1897 [Abstract] [Full Text]  
  • Wang, Z., Day, N., Trifillis, P., Kiledjian, M. (1999). An mRNA Stability Complex Functions with Poly(A)-Binding Protein To Stabilize mRNA In Vitro. Mol. Cell. Biol. 19: 4552-4560 [Abstract] [Full Text]  
  • Chkheidze, A. N., Lyakhov, D. L., Makeyev, A. V., Morales, J., Kong, J., Liebhaber, S. A. (1999). Assembly of the alpha -Globin mRNA Stability Complex Reflects Binary Interaction between the Pyrimidine-Rich 3' Untranslated Region Determinant and Poly(C) Binding Protein alpha CP. Mol. Cell. Biol. 19: 4572-4581 [Abstract] [Full Text]  
  • Zhao, J., Hyman, L., Moore, C. (1999). Formation of mRNA 3' Ends in Eukaryotes: Mechanism, Regulation, and Interrelationships with Other Steps in mRNA Synthesis. Microbiol. Mol. Biol. Rev. 63: 405-445 [Abstract] [Full Text]  
  • Sokolowski, M., Furneaux, H., Schwartz, S. (1999). The Inhibitory Activity of the AU-Rich RNA Element in the Human Papillomavirus Type 1 Late 3' Untranslated Region Correlates with Its Affinity for the elav-Like HuR Protein. J. Virol. 73: 1080-1091 [Abstract] [Full Text]  
  • Paulding, W. R., Czyzyk-Krzeska, M. F. (1999). Regulation of Tyrosine Hydroxylase mRNA Stability by Protein-binding, Pyrimidine-rich Sequence in the 3'-Untranslated Region. J. Biol. Chem. 274: 2532-2538 [Abstract] [Full Text]  
  • Shih, S.-C., Claffey, K. P. (1999). Regulation of Human Vascular Endothelial Growth Factor mRNA Stability in Hypoxia by Heterogeneous Nuclear Ribonucleoprotein L. J. Biol. Chem. 274: 1359-1365 [Abstract] [Full Text]  
  • Heise, T., Guidotti, L. G., Cavanaugh, V. J., Chisari, F. V. (1999). Hepatitis B Virus RNA-Binding Proteins Associated with Cytokine-Induced Clearance of Viral RNA from the Liver of Transgenic Mice. J. Virol. 73: 474-481 [Abstract] [Full Text]  
  • DeMaria, C. T., Sun, Y., Long, L., Wagner, B. J., Brewer, G. (1997). Structural Determinants in AUF1 Required for High Affinity Binding to A + U-rich Elements. J. Biol. Chem. 272: 27635-27643 [Abstract] [Full Text]  
  • D'Orso, I., Frasch, A. C. C. (2001). Functionally Different AU- and G-rich cis-Elements Confer Developmentally Regulated mRNA Stability in Trypanosoma cruzi by Interaction with Specific RNA-binding Proteins. J. Biol. Chem. 276: 15783-15793 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»