This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sandaltzopoulos, R.
Right arrow Articles by Becker, P. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sandaltzopoulos, R.
Right arrow Articles by Becker, P. B.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Jan 1998, 361-367, Vol 18, No. 1
Copyright © 1998, American Society for Microbiology

Heat shock factor increases the reinitiation rate from potentiated chromatin templates

R Sandaltzopoulos and PB Becker
Gene Expression Programme, European Molecular Biology Laboratory, Heidelberg, Germany.

Transcription by RNA polymerase II is highly regulated at the level of initiation and elongation. Well-documented transcription activation mechanisms, such as the recruitment of TFIID and TFIIB, control the early phases of preinitiation complex formation. The heat shock genes provide an example for transcriptional regulation at a later step: in nuclei TFIID can be detected at the TATA box prior to heat induction. Using cell-free systems for chromatin reconstitution and transcription, we have analyzed the mechanisms by which heat shock factor (HSF) increases transcription of heat shock genes in chromatin. HSF affected transcription of naked DNA templates in multiple ways: (i) by speeding up the rate of preinitiation complex formation, (ii) by increasing the number of productive templates, and (iii) by increasing the reinitiation rate. Under the more physiological conditions of potentiated chromatin templates, HSF affected only the reinitiation rate. Activator-dependent reinitiation of transcription, obviating the slow assembly of the TFIID-TFIIA complex on a promoter, may be especially crucial for genes requiring a fast response to inducers.


This article has been cited by other articles:

  • Yun, K., So, J.-S., Jash, A., Im, S.-H. (2009). Lymphoid Enhancer Binding Factor 1 Regulates Transcription through Gene Looping. J. Immunol. 183: 5129-5137 [Abstract] [Full Text]  
  • Knuesel, M. T., Meyer, K. D., Bernecky, C., Taatjes, D. J. (2009). The human CDK8 subcomplex is a molecular switch that controls Mediator coactivator function. Genes Dev. 23: 439-451 [Abstract] [Full Text]  
  • Sprouse, R. O., Karpova, T. S., Mueller, F., Dasgupta, A., McNally, J. G., Auble, D. T. (2008). Regulation of TATA-binding protein dynamics in living yeast cells. Proc. Natl. Acad. Sci. USA 105: 13304-13308 [Abstract] [Full Text]  
  • Morales, V., Regnard, C., Izzo, A., Vetter, I., Becker, P. B. (2005). The MRG Domain Mediates the Functional Integration of MSL3 into the Dosage Compensation Complex. Mol. Cell. Biol. 25: 5947-5954 [Abstract] [Full Text]  
  • Acevedo, M. L., Kraus, W. L. (2003). Mediator and p300/CBP-Steroid Receptor Coactivator Complexes Have Distinct Roles, but Function Synergistically, during Estrogen Receptor {alpha}-Dependent Transcription with Chromatin Templates. Mol. Cell. Biol. 23: 335-348 [Abstract] [Full Text]  
  • Reeves, W. M., Hahn, S. (2003). Activator-Independent Functions of the Yeast Mediator Sin4 Complex in Preinitiation Complex Formation and Transcription Reinitiation. Mol. Cell. Biol. 23: 349-358 [Abstract] [Full Text]  
  • Ma, B., Hernandez, N. (2002). Redundant Cooperative Interactions for Assembly of a Human U6 Transcription Initiation Complex. Mol. Cell. Biol. 22: 8067-8078 [Abstract] [Full Text]  
  • Ainbinder, E., Revach, M., Wolstein, O., Moshonov, S., Diamant, N., Dikstein, R. (2002). Mechanism of Rapid Transcriptional Induction of Tumor Necrosis Factor Alpha-Responsive Genes by NF-{kappa}B. Mol. Cell. Biol. 22: 6354-6362 [Abstract] [Full Text]  
  • Upadhyaya, A. B., Khan, M., Mou, T.-C., Junker, M., Gray, D. M., DeJong, J. (2002). The Germ Cell-specific Transcription Factor ALF. STRUCTURAL PROPERTIES AND STABILIZATION OF THE TATA-BINDING PROTEIN (TBP)-DNA COMPLEX. J. Biol. Chem. 277: 34208-34216 [Abstract] [Full Text]  
  • Cheung, E., Zarifyan, A. S., Kraus, W. L. (2002). Histone H1 Represses Estrogen Receptor {alpha} Transcriptional Activity by Selectively Inhibiting Receptor-Mediated Transcription Initiation. Mol. Cell. Biol. 22: 2463-2471 [Abstract] [Full Text]  
  • Gawlas, K., Stunnenberg, H. G. (2001). Differential transcription of the orphan receptor ROR{beta} in nuclear extracts derived from Neuro2A and HeLa cells. Nucleic Acids Res 29: 3424-3432 [Abstract] [Full Text]  
  • Hume, D. A. (2000). Probability in transcriptional regulation and its implications for leukocyte differentiation and inducible gene expression. Blood 96: 2323-2328 [Abstract] [Full Text]  
  • Nogales, E (2000). Recent structural insights into transcription preinitiation complexes. J. Cell Sci. 113: 4391-4397 [Abstract]  
  • Yie, J., Senger, K., Thanos, D. (1999). Mechanism by which the IFN-beta enhanceosome activates transcription. Proc. Natl. Acad. Sci. USA 96: 13108-13113 [Abstract] [Full Text]  
  • Raschke, E. E., Albert, T., Eick, D. (1999). Transcriptional Regulation of the Ig{kappa} Gene by Promoter-Proximal Pausing of RNA Polymerase II. J. Immunol. 163: 4375-4382 [Abstract] [Full Text]  
  • Solow, S. P., Lezina, L., Lieberman, P. M. (1999). Phosphorylation of TFIIA Stimulates TATA Binding Protein-TATA Interaction and Contributes to Maximal Transcription and Viability in Yeast. Mol. Cell. Biol. 19: 2846-2852 [Abstract] [Full Text]  
  • Ranish, J. A., Yudkovsky, N., Hahn, S. (1999). Intermediates in formation and activity of the RNA polymerase II preinitiation complex: holoenzyme recruitment and a postrecruitment role for the TATA box and TFIIB. Genes Dev. 13: 49-63 [Abstract] [Full Text]  
  • HAHN, S. (1998). Activation and the Role of Reinitiation in the Control of Transcription by RNA Polymerase II. Cold Spring Harb Symp Quant Biol 63: 181-188 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»