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Molecular and Cellular Biology, October 1998, p. 5961-5969, Vol. 18, No. 10
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

myc Boxes, Which Are Conserved in myc Family Proteins, Are Signals for Protein Degradation via the Proteasome

Elizabeth M. Flinn,* C. Magnus C. Busch, and Anthony P. H. Wright

Karolinska Institute, Department of Biosciences, NOVUM, S-14157 Huddinge, Sweden

Received 20 March 1998/Returned for modification 23 April 1998/Accepted 30 June 1998

Cellular levels of the rapidly degraded c-myc protein play an important role in determining the proliferation status of cells. Increased levels of c-myc are frequently associated with rapidly proliferating tumor cells. We show here that myc boxes I and II, found in the N termini of all members of the myc protein family, function to direct the degradation of the c-myc protein. Both myc boxes I and II contain sufficient information to independently direct the degradation of otherwise stably expressed proteins to which they are fused. At least part of the myc box-directed degradation occurs via the proteasome. The mechanism of myc box-directed degradation appears to be conserved between yeast and mammalian cells. Our results suggest that the myc boxes may play an important role in regulating the level and activity of the c-myc protein.

* Corresponding author. Mailing address: Karolinska Institute, Department of Biosciences, NOVUM, 141 57 Huddinge, Sweden. Phone: 46 (0)8 608 9164. Fax: 46 (0)8 774 5538. E-mail: elizabeth.flinn{at}cbt.ki.se.

Molecular and Cellular Biology, October 1998, p. 5961-5969, Vol. 18, No. 10
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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