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Molecular and Cellular Biology, November 1998, p. 6213-6223, Vol. 18, No. 11
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

MFR, a Putative Receptor Mediating the Fusion of Macrophages

Charles Saginario,1 Hyacinth Sterling,1,2 Cornelius Beckers,3 Ruji Kobayashi,4 Michele Solimena,5 Elisabetta Ullu,1,3 and Agnès Vignery1,2,*

Department of Cell Biology,1 Department of Medicine, Section of Infectious Diseases,3 Department of Medicine, Section of Endocrinology,5 and Department of Orthopaedics and Rehabilitation,2 Yale University School of Medicine, New Haven, Connecticut, and Cold Spring Harbor Laboratory, Cold Spring Harbor, New York4

Received 26 March 1998/Returned for modification 15 July 1998/Accepted 31 July 1998

We had previously identified a macrophage surface protein whose expression is highly induced, transient, and specific, as it is restricted to actively fusing macrophages in vitro and in vivo. This protein is recognized by monoclonal antibodies that block macrophage fusion. We have now purified this protein and cloned its corresponding cDNA. This protein belongs to the superfamily of immunoglobulins and is similar to immune antigen receptors such as the T-cell receptor, B-cell receptor, and viral receptors such as CD4. We have therefore named this protein macrophage fusion receptor (MFR). We show that the extracellular domain of MFR prevents fusion of macrophages in vitro and therefore propose that MFR belongs to the fusion machinery of macrophages. MFR is identical to SHPS-1 and BIT and is a homologue of P84, SIRPalpha , and MyD-1, all of which have been recently cloned and implicated in cell signaling and cell-cell interaction events.

* Corresponding author. Mailing address: Yale University School of Medicine, Department of Orthopaedics and Rehabilitation, 333 Cedar St., New Haven, CT 06510. Phone: (203) 785-5968. Fax: (203) 737-2701. E-mail: agnes.vignery{at}yale.edu.

Molecular and Cellular Biology, November 1998, p. 6213-6223, Vol. 18, No. 11
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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