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Molecular and Cellular Biology, December 1998, p. 7360-7370, Vol. 18, No. 12
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cell Cycle Regulation of the Saccharomyces cerevisiae Polo-Like Kinase Cdc5p

Liang Cheng, Linda Hunke, and Christopher F. J. Hardy*

Department of Cell Biology and Physiology and Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110

Received 23 June 1998/Returned for modification 20 August 1998/Accepted 10 September 1998

Progression through and completion of mitosis require the actions of the evolutionarily conserved Polo kinase. We have determined that the levels of Cdc5p, a Saccharomyces cerevisiae member of the Polo family of mitotic kinases, are cell cycle regulated. Cdc5p accumulates in the nuclei of G2/M-phase cells, and its levels decline dramatically as cells progress through anaphase and begin telophase. We report that Cdc5p levels are sensitive to mutations in key components of the anaphase-promoting complex (APC). We have determined that Cdc5p-associated kinase activity is restricted to G2/M and that this activity is posttranslationally regulated. These results further link the actions of the APC to the completion of mitosis and suggest possible roles for Cdc5p during progression through and completion of mitosis.

* Corresponding author. Mailing address: Department of Genetics, Washington University School of Medicine, Box 8232, 660 South Euclid Ave., St. Louis, MO 63110. Phone: (314) 747-1808. Fax: (314) 362-7463. E-mail: chardy{at}cellbio.wustl.edu.

Molecular and Cellular Biology, December 1998, p. 7360-7370, Vol. 18, No. 12
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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