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Mol Cell Biol, March 1998, p. 1622-1634, Vol. 18, No. 3
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Epidermal Growth Factor Receptor and the Adaptor Protein p52Shc Are Specific Substrates of T-Cell Protein Tyrosine Phosphatase

Tony Tiganis,1,dagger Anton M. Bennett,1,Dagger Kodimangalam S. Ravichandran,2 and Nicholas K. Tonks1,*

Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724,1 and Bierne Carter Center for Immunology Research and Department of Microbiology, University of Virginia, Charlottesville, Virginia 229082

Received 21 July 1997/Returned for modification 2 September 1997/Accepted 14 November 1997

T-cell protein tyrosine phosphatase (TCPTP) exists as two forms generated by alternative splicing: a 48-kDa endoplasmic reticulum (ER)-associated form (TC48) and a 45-kDa nuclear form (TC45). To identify TCPTP substrates, we have generated substrate-trapping mutants, in which the invariant catalytic acid of TCPTP (D182) is mutated to alanine. The TCPTP D182A substrate-trapping mutants were transiently overexpressed in COS cells, and their ability to form complexes with tyrosine-phosphorylated (pTyr) proteins was assessed. No pTyr proteins formed complexes with wild-type TCPTP. In contrast, TC48-D182A formed a complex in the ER with pTyr epidermal growth factor receptor (EGFR). In response to EGF, TC45-D182A exited the nucleus and accumulated in the cytoplasm, where it bound pTyr proteins of ~50, 57, 64, and 180 kDa. Complex formation was disrupted by vanadate, highlighting the importance of the PTP active site in the interaction and supporting the characterization of these proteins as substrates. Of these TC45 substrates, the ~57- and 180-kDa proteins were identified as p52Shc and EGFR, respectively. We examined the effects of TC45 on EGFR signaling and observed that it did not modulate EGF-induced activation of p42Erk2. However, TC45 inhibited the EGF-induced association of p52Shc with Grb2, which was attributed to the ability of the PTP to recognize specifically p52Shc phosphorylated on Y239. These results indicate that TC45 recognizes not only selected substrates in a cellular context but also specific sites within substrates and thus may regulate discrete signaling events.

* Corresponding author. Mailing address: 1 Bungtown Road, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724-2208. Phone: (516) 367-8846. Fax: (516) 367-6812. E-mail: tonks{at}cshl.org.

dagger Present address: St. Vincent's Institute of Medical Research, Fitzroy, Victoria 3065, Australia.

Dagger Present address: Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520.




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