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Mol Cell Biol, April 1998, p. 1855-1865, Vol. 18, No. 4
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Dbp6p Is an Essential Putative ATP-Dependent RNA Helicase Required for 60S-Ribosomal-Subunit Assembly in Saccharomyces cerevisiae

Dieter Kressler,1,* Jesús de la Cruz,1 Manuel Rojo,2 and Patrick Linder1

Département de Biochimie Médicale, Centre Médical Universitaire,1 and Département de Biochimie, Sciences II,2 Université de Genève, 1211 Geneva 4, Switzerland

Received 31 October 1997/Returned for modification 21 November 1997/Accepted 30 December 1997

A previously uncharacterized Saccharomyces cerevisiae open reading frame, YNR038W, was analyzed in the context of the European Functional Analysis Network. YNR038W encodes a putative ATP-dependent RNA helicase of the DEAD-box protein family and was therefore named DBP6 (DEAD-box protein 6). Dbp6p is essential for cell viability. In vivo depletion of Dbp6p results in a deficit in 60S ribosomal subunits and the appearance of half-mer polysomes. Pulse-chase labeling of pre-rRNA and steady-state analysis of pre-rRNA and mature rRNA by Northern hybridization and primer extension show that Dbp6p depletion leads to decreased production of the 27S and 7S precursors, resulting in a depletion of the mature 25S and 5.8S rRNAs. Furthermore, hemagglutinin epitope-tagged Dbp6p is detected exclusively within the nucleolus. We propose that Dbp6p is required for the proper assembly of preribosomal particles during the biogenesis of 60S ribosomal subunits, probably by acting as an rRNA helicase.

* Corresponding author. Mailing address: Département de Biochimie Médicale, Centre Médical Universitaire, Université de Genève, 1 rue Michel-Servet, CH-1211 Geneva 4, Switzerland. Phone: 41 22 702 55 08. Fax: 41 22 702 55 02. E-mail: dieter.kressler{at}medecine.unige.ch.




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