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Mol Cell Biol, May 1998, p. 2455-2461, Vol. 18, No. 5
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Chromatin Remodeling Mediated by Drosophila GAGA Factor and ISWI Activates fushi tarazu Gene Transcription In Vitro

Masahiro Okada and Susumu Hirose*

Department of Genetics, The Graduate University for Advanced Studies, and Department of Developmental Genetics, National Institute of Genetics, Mishima, Shizuoka-ken 411, Japan

Received 30 December 1997/Returned for modification 6 February 1998/Accepted 24 February 1998

GAGA factor is known to remodel the chromatin structure in concert with nucleosome-remodeling factor NURF in a Drosophila embryonic S150 extract. The promoter region of the Drosophila fushi tarazu (ftz) gene carries several binding sites for GAGA factor. Both the GAGA factor-binding sites and GAGA factor per se are necessary for the proper expression of ftz in vivo. We observed transcriptional activation of the ftz gene when a preassembled chromatin template was incubated with GAGA factor and the S150 extract. The chromatin structure within the ftz promoter was specifically disrupted by incubation of the preassembled chromatin with GAGA factor and the S150 extract. Both transcriptional activation and chromatin disruption were blocked by an antiserum raised against ISWI or by base substitutions in the GAGA factor-binding sites in the ftz promoter region. These results demonstrate that GAGA factor- and ISWI-mediated disruption of the chromatin structure within the promoter region of ftz activates transcription on the chromatin template.

* Corresponding author. Mailing address: Department of Developmental Genetics, National Institute of Genetics, Mishima, Shizuoka-ken 411, Japan. Phone: 81-559-81-6771. Fax: 81-559-81-6776. E-mail: shirose{at}lab.nig.ac.jp.

Mol Cell Biol, May 1998, p. 2455-2461, Vol. 18, No. 5
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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