This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Farr, K. A.
Right arrow Articles by Hoyt, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Farr, K. A.
Right arrow Articles by Hoyt, M. A.

 Previous Article  |  Next Article 

Mol Cell Biol, May 1998, p. 2738-2747, Vol. 18, No. 5
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Bub1p Kinase Activates the Saccharomyces cerevisiae Spindle Assembly Checkpoint

Katie A. Farr and M. Andrew Hoyt*

Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218

Received 10 December 1997/Returned for modification 26 January 1998/Accepted 23 February 1998

Saccharomyces cerevisiae BUB1 encodes a protein kinase required for spindle assembly checkpoint function. In the presence of spindle damage, BUB1 is required to prevent cell cycle progression into anaphase. We have identified a dominantly acting BUB1 allele that appears to activate the spindle assembly checkpoint pathway in cells with undamaged spindles. High-level expression of BUB1-5 did not cause detectable spindle damage, yet it delayed yeast cells in mitosis at a stage following bipolar spindle assembly but prior to anaphase spindle elongation. Delayed cells possessed a G2 DNA content and elevated Clb2p mitotic cyclin levels. Unlike cells delayed in mitosis by spindle damage or MPS1 kinase overexpression, hyperphosphorylated forms of the Mad1p checkpoint protein did not accumulate. Similar to cells overexpressing MPS1, the BUB1-5 delay was dependent upon the functions of the other checkpoint genes, including BUB2 and BUB3 and MAD1, MAD2, and MAD3. We found that the mitotic delay caused by BUB1-5 or MPS1 overexpression was interdependent upon the function of the other. This suggests that the Bub1p and Mps1p kinases act together at an early step in generating the spindle damage signal.

* Corresponding author. Mailing address: Department of Biology, The Johns Hopkins University, Baltimore, MD 21218. Phone: (410) 516-7299. Fax: (410) 516-5213. E-mail: hoyt{at}jhu.edu.

Mol Cell Biol, May 1998, p. 2738-2747, Vol. 18, No. 5
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Qi, W., Yu, H. (2007). KEN-Box-dependent Degradation of the Bub1 Spindle Checkpoint Kinase by the Anaphase-promoting Complex/Cyclosome. J. Biol. Chem. 282: 3672-3679 [Abstract] [Full Text]  
  • Guo, C., Wu, G., Chin, J. L., Bauman, G., Moussa, M., Wang, F., Greenberg, N. M., Taylor, S. S., Xuan, J. W. (2006). Bub1 Up-Regulation and Hyperphosphorylation Promote Malignant Transformation in SV40 Tag-Induced Transgenic Mouse Models. Mol Cancer Res 4: 957-969 [Abstract] [Full Text]  
  • Li, J.-m., Li, Y., Elledge, S. J. (2005). Genetic Analysis of the Kinetochore DASH Complex Reveals an Antagonistic Relationship with the Ras/Protein Kinase A Pathway and a Novel Subunit Required for Ask1 Association. Mol. Cell. Biol. 25: 767-778 [Abstract] [Full Text]  
  • Vigneron, S., Prieto, S., Bernis, C., Labbe, J.-C., Castro, A., Lorca, T. (2004). Kinetochore Localization of Spindle Checkpoint Proteins: Who Controls Whom?. Mol. Biol. Cell 15: 4584-4596 [Abstract] [Full Text]  
  • Gillett, E. S., Espelin, C. W., Sorger, P. K. (2004). Spindle checkpoint proteins and chromosome-microtubule attachment in budding yeast. JCB 164: 535-546 [Abstract] [Full Text]  
  • Tunquist, B. J., Eyers, P. A., Chen, L. G., Lewellyn, A. L., Maller, J. L. (2003). Spindle checkpoint proteins Mad1 and Mad2 are required for cytostatic factor-mediated metaphase arrest. JCB 163: 1231-1242 [Abstract] [Full Text]  
  • Kerscher, O., Crotti, L. B., Basrai, M. A. (2003). Recognizing Chromosomes in Trouble: Association of the Spindle Checkpoint Protein Bub3p with Altered Kinetochores and a Unique Defective Centromere. Mol. Cell. Biol. 23: 6406-6418 [Abstract] [Full Text]  
  • Iouk, T., Kerscher, O., Scott, R. J., Basrai, M. A., Wozniak, R. W. (2002). The yeast nuclear pore complex functionally interacts with components of the spindle assembly checkpoint. JCB 159: 807-819 [Abstract] [Full Text]  
  • Warren, C. D., Brady, D. M., Johnston, R. C., Hanna, J. S., Hardwick, K. G., Spencer, F. A. (2002). Distinct Chromosome Segregation Roles for Spindle Checkpoint Proteins. Mol. Biol. Cell 13: 3029-3041 [Abstract] [Full Text]  
  • Chen, R.-H. (2002). BubR1 is essential for kinetochore localization of other spindle checkpoint proteins and its phosphorylation requires Mad1. JCB 158: 487-496 [Abstract] [Full Text]  
  • Abruzzi, K. C., Magendantz, M., Solomon, F. (2002). An {alpha}-Tubulin Mutant Demonstrates Distinguishable Functions Among the Spindle Assembly Checkpoint Genes in Saccharomyces cerevisiae. Genetics 161: 983-994 [Abstract] [Full Text]  
  • Millband, D. N., Hardwick, K. G. (2002). Fission Yeast Mad3p Is Required for Mad2p To Inhibit the Anaphase-Promoting Complex and Localizes to Kinetochores in a Bub1p-, Bub3p-, and Mph1p-Dependent Manner. Mol. Cell. Biol. 22: 2728-2742 [Abstract] [Full Text]  
  • Li, Y., Bachant, J., Alcasabas, A. A., Wang, Y., Qin, J., Elledge, S. J. (2002). The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev. 16: 183-197 [Abstract] [Full Text]  
  • Hildebrandt, E. R., Hoyt, M. A. (2001). Cell Cycle-dependent Degradation of the Saccharomyces cerevisiae Spindle Motor Cin8p Requires APCCdh1 and a Bipartite Destruction Sequence. Mol. Biol. Cell 12: 3402-3416 [Abstract] [Full Text]  
  • Sharp-Baker, H., Chen, R.-H. (2001). Spindle Checkpoint Protein Bub1 Is Required for Kinetochore Localization of Mad1, Mad2, Bub3, and Cenp-E, Independently of Its Kinase Activity. JCB 153: 1239-1250 [Abstract] [Full Text]  
  • Olesen, S. H., Thykjaer, T., Orntoft, T. F. (2001). Mitotic checkpoint genes hBUB1, hBUB1B, hBUB3 and TTK in human bladder cancer, screening for mutations and loss of heterozygosity. Carcinogenesis 22: 813-815 [Abstract] [Full Text]  
  • Kalitsis, P., Earle, E., Fowler, K. J., Choo, K.H. A. (2000). Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis. Genes Dev. 14: 2277-2282 [Abstract] [Full Text]  
  • Murakumo, Y., Roth, T., Ishii, H., Rasio, D., Numata, S.-i., Croce, C. M., Fishel, R. (2000). A Human REV7 Homolog That Interacts with the Polymerase zeta Catalytic Subunit hREV3 and the Spindle Assembly Checkpoint Protein hMAD2. J. Biol. Chem. 275: 4391-4397 [Abstract] [Full Text]  
  • Li, W., Lan, Z., Wu, H., Wu, S., Meadows, J., Chen, J., Zhu, V., Dai, W. (1999). BUBR1 Phosphorylation Is Regulated during Mitotic Checkpoint Activation. Cell Growth Differ. 10: 769-775 [Abstract] [Full Text]  
  • Chen, R.-H., Brady, D. M., Smith, D., Murray, A. W., Hardwick, K. G. (1999). The Spindle Checkpoint of Budding Yeast Depends on a Tight Complex between the Mad1 and Mad2 Proteins. Mol. Biol. Cell 10: 2607-2618 [Abstract] [Full Text]  
  • Basu, J., Bousbaa, H., Logarinho, E., Li, Z., Williams, B. C., Lopes, C., Sunkel, C. E., Goldberg, M. L. (1999). Mutations in the Essential Spindle Checkpoint Gene bub1 Cause Chromosome Missegregation and Fail to Block Apoptosis in Drosophila. JCB 146: 13-28 [Abstract] [Full Text]  
  • Li, R. (1999). Bifurcation of the mitotic checkpoint pathway in budding yeast. Proc. Natl. Acad. Sci. USA 96: 4989-4994 [Abstract] [Full Text]  
  • Bernard, P., Hardwick, K., Javerzat, J.-P. (1998). Fission Yeast Bub1 Is a Mitotic Centromere Protein Essential for the Spindle Checkpoint and the Preservation of Correct Ploidy through Mitosis. JCB 143: 1775-1787 [Abstract] [Full Text]  
  • Mendenhall, M. D., Hodge, A. E. (1998). Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62: 1191-1243 [Abstract] [Full Text]  
  • Chen, R.-H., Shevchenko, A., Mann, M., Murray, A. W. (1998). Spindle Checkpoint Protein Xmad1 Recruits Xmad2 to Unattached Kinetochores. JCB 143: 283-295 [Abstract] [Full Text]  
  • Wassmann, K., Benezra, R. (1998). Mad2 transiently associates with an APC/p55Cdc complex during mitosis. Proc. Natl. Acad. Sci. USA 95: 11193-11198 [Abstract] [Full Text]  
  • Taylor, S. S., Ha, E., McKeon, F. (1998). The Human Homologue of Bub3 Is Required for Kinetochore Localization of Bub1 and a Mad3/Bub1-related Protein Kinase. JCB 142: 1-11 [Abstract] [Full Text]  
  • Wang, X., Babu, J. R., Harden, J. M., Jablonski, S. A., Gazi, M. H., Lingle, W. L., de Groen, P. C., Yen, T. J., van Deursen, J. M. A. (2001). The Mitotic Checkpoint Protein hBUB3 and the mRNA Export Factor hRAE1 Interact with GLE2p-binding Sequence (GLEBS)-containing Proteins. J. Biol. Chem. 276: 26559-26567 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»